Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Breeding of a Recessive Soybean Genotype (titirs2rs2) with Green Cotyledons and Black Seed Coats

titirs2rs2 열성 유전자형을 가진 속푸른 검정콩 계통 육성

  • Choi, Sang Woo (Department of Agronomy, Gyeongsang National University) ;
  • Kim, Jin A (Department of Agronomy, Gyeongsang National University) ;
  • Shim, Sang In (Department of Agronomy, Gyeongsang National University) ;
  • Kim, Min Chul (Department of Agronomy, Gyeongsang National University) ;
  • Chung, Jong Il (Department of Agronomy, Gyeongsang National University)
  • Received : 2018.08.05
  • Accepted : 2018.10.06
  • Published : 2019.02.28
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Abstract

Soybean [Glycine max (L.) Merr.] is grown worldwide for its high protein and oil content. Anthocyanins from black soybean seed coats are known to have many pharmaceutical effects. Soybean cultivars with large seed sizes and black seed coats are needed by soybean farmers. However, antinutritional factors, like protein, stachyose, and Kunitz trypsin inhibitor (KTI) exist in raw mature soybeans. Genetic elimination or reduction of these components is needed in soybean breeding. The objective of this research was to develop new a soybean strain with black seed coats and green cotyledons that was KTI protein free and low in stachyose. Six parents were used. The presence or absence of KTI protein was detected using the Western blot technique. The content of stachyose in mature seeds was detected using HPLC. One new strain was selected from 11 $F_2$ plants with black seed coats and green cotyledons that lacked KTI protein. The new strain had black seed coats and green cotyledons and was KTI protein free and low in stachyose. The plant height of the new strain was 66 cm, and its 100-seed weight was 28.4 g. The stachyose content of the new strain was 2.59 g/kg. The new strain developed in this research will be used to develop new cultivars that are KTI protein free and low in stachyose.

콩[Glycine max (L.) Merr. 2n=40]은 국내에서 벼 다음으로 많이 재배되고 있는 식량작물이며 성숙 콩 종실에는 3대 영양소외 페놀성 화합물, 이소플라본, 사포닌, 루테인 토코페롤, 피틴산, 루테인, 안토시아닌등 생리활성 물질이 다양하게 존재하지만 기능성과 가공 적성 및 품질을 저해시키는 성분들도 다수 존재한다. 성숙 콩 종실에서 품질, 기능성 및 가공 적성을 저하시키는 KTI 단백질이 없으면서 난소화성 올리고당인 stachyose 함량이 낮은 속 푸른 검정콩 유전자형을 육성하기 위하여 진행되었다. 3개의 모본으로 육종집단을 창성하여 검정종피, 녹색자엽 및 KTI 단백질이 없는 11개의 $F_2$ 종자를 얻었고 stachyose 함량이 낮은 2개의 계통을 선발하여 농업형질 평가를 통하여 검정 종피, 녹색자엽, stachyose 함량이 낮은 titirs2rs2 유전자형을 가진 계통을 선발하였다. 선발된 계통의 초장은 66 cm 정도였으며 백립중은 28.4 g으로 대립이었으며 종피색과 제색은 모두 검정색이었고 성숙 자엽색은 녹색이었다. 선발된 계통의 stachyose 함량은 일반품종(13.01-16.81 g/kg)보다 매우 낮은 2.59(g/kg)이었다. 본 연구를 통하여 선발된 계통은 Kunitz Trypsine Inhibitor (KTI) 단백질이 없으며 난소화성 올리고당인 stachyose 성분의 함량이 낮고 검정종피와 녹색자엽을 가진 기능성 고품질 유색콩 육성을 위한 중간모본으로 이용될 수 있을 것으로 사료되었다.

Keywords

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Fig. 1. Scheme for developing new soybean strain with black seed coat, green cotyledon, Kunitz Trypsin Inhibitor (KTI) protein free, and low content of stachyose.

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Fig. 2. Confirmation of Kunitz trypsin inhibitor (KTI) protein free in the parent and new strain. C:Seoritae, S:new strain (black seed coat, green cotyledon, and low content of stachyose).

Table 1. Seed coat color, cotyledon, Kunitz Trypsin Inhibitor (KTI) protein, and stachyose content of three parents used in this experiment

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Table 2. Agronomic traits of new strain selected in this experiment

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Table 3. Content of stachyose for five cultivars and one new strain selected in this experiment

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