Parasites, Hosts and Diseases

  • 제57권1호
  • /
  • Pages.61-67
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  • 2019
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  • 2982-5164(pISSN)
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  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
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Multi-Epitope Fusion Protein Eg mefAg-1 as a Serodiagnostic Candidate for Cystic Echinococcosis in Sheep

  • Tianli, Liu (College of Animal Science & Technology, Shihezi University) ;
  • Xifeng, Wang (College of Animal Science & Technology, Shihezi University) ;
  • Zhenzhong, Tian (College of Animal Science & Technology, Shihezi University) ;
  • Lixia, Wang (College of Animal Science & Technology, Shihezi University) ;
  • Xingxing, Zhang (State Key Laboratory for Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Science) ;
  • Jun, Qiao (College of Animal Science & Technology, Shihezi University) ;
  • Qingling, Meng (College of Animal Science & Technology, Shihezi University) ;
  • Shasha, Gong (College of Animal Science & Technology, Shihezi University) ;
  • Ying, Chen (College of Animal Science & Technology, Shihezi University) ;
  • Xuepeng, Cai (State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences)
  • 투고 : 2018.05.01
  • 심사 : 2019.01.30
  • 발행 : 2019.02.28
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초록

Cystic echinococcosis (CE) in sheep is a hazardous zoonotic parasitic disease that is caused by Echinococcus granulosus (Eg). At present, serological test is an important diagnostic method for Eg infection in domestic animals. Here, a fusion protein Eg mefAg-1 harboring 8 dominant B-cell epitopes of Eg such as antigen B, tetraspanin 1, tetraspanin 6, reticulon and Eg95 was produced in E. coli and evaluated for CE in sheep by indirect ELISA. Eg mefAg-1 showed in ELISA a high sensitivity (93.41%) and specificity (99.31%), with a coincidence rate of 97.02%. Overall, it is suggested that the Eg mefAg-1 could be a potential antigen candidate for CE serodiagnosis in sheep.

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참고문헌

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피인용 문헌

  1. Detection of Echinococcus granulosus sensu lato infection by using extracts derived from a protoscoleces G1 cell line vol.41, pp.12, 2019, https://doi.org/10.1111/pim.12674