Korea Journal of Cosmetic Science

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Inhibitory Effect of Haplamine on Melanosome Transport and Its Mechanism of Action

  • Lee, Kyung Rhim (Department of Genetic Engineering & Graduate School of Biotechnology, Kyung Hee University) ;
  • Myung, Cheol Hwan (Department of Genetic Engineering & Graduate School of Biotechnology, Kyung Hee University) ;
  • Hwang, Jae Sung (Department of Genetic Engineering & Graduate School of Biotechnology, Kyung Hee University)
  • Received : 2019.12.22
  • Accepted : 2019.12.27
  • Published : 2019.12.30
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Abstract

Melanosomes are specific melanin-containing intracellular organelles of epidermal melanocytes. In epidermal melanocytes, there are three kinds of key player proteins. Rab27a, melanophilin or Slac2-a and Myosin 5a form a tripartite complex connects the melanosome. Mature melanosomes make movements through the tripartite protein complex along actin filaments.In this study, we found that the haplamine (6-Methoxyflindersine) induced melanosome aggregation around the nucleus in epidermal melanocyte. In an attempt to elucidate the inhibitory effect of haplamine on melanosome transport, effect of haplamineon the expression level of Rab27a, melanophilin and Myosin 5a was measured. The results indicated that haplamine up to 5��M effectively suppressed mRNA and protein expression level of melanophilin.To determine the upstream regulator of melanophilin regulated by haplamine, we checked the level of MITF, c-JUN and USF1. Those are possible transcription factor of melanophilin. Among them,treatment of USF1 siRNA decreased mRNA and protein expression level of USF1 as well as melanophilin. Also, treatment of haplamine decreased mRNA and protein expression level of melanophilin as well as USF1 in a dose-dependent manner. Consequently, we found the inhibitory effect of haplamine on melanosome transport in melan-a melanocyte. Treatment of haplamine reduced melanophilin expression level which is a key protein of melanosome transport. We identified that USF1 could be a major transcription factor of melanophilin regulated by haplamine.

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References

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