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Occurrence of Phytopythium vexans Causing Stem Rot on Anthurium andraeanum in Korea

  • Park, Mi-Jeong (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Back, Chang-Gi (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Park, Jong-Han (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration)
  • Received : 2019.12.20
  • Accepted : 2019.12.20
  • Published : 2019.12.31

Abstract

In 2017, wilting symptom was observed on seedlings of Anthurium andraeranum in Youngin, Korea. Brownish lesions with water soaking were developed on the stems and roots of the infected plants. The stems and leaves wilted and finally died. One fungal isolate was obtained in pure culture. Morphological features and nucleotide sequences of internal transcribed spacer rDNA and cytochrome oxidase subunit II mt DNA were analyzed. The results of this study indicated that the fungus is identified as Phytopythium vexans. Pathogenicity tests showed the isolate was pathogenic to the seedlings of A. andraeanum. To our knowledge, this is the first report of P. vexans causing stem rot on A. andraeanum in Korea.

Keywords

References

  1. White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungus ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR protocols: a guide to methods and applications. San Diego: Academic Press; 1990. p. 315-22.
  2. Choi YJ, Beakes G, Glockling S, Kruse J, Nam B, Nigrelli L, Ploch S, Shin HD, Shivas RG, Telle S, Voglmayr H, Thines M. Towards a universal barcode of oomycetes - a comparison of the cox1 and cox2 loci. Mol Ecol Resour 2015;15:1275-88. https://doi.org/10.1111/1755-0998.12398
  3. Tamura K, Stecher G, Tamua K. MEGA7: molecular evolutionary genetics analysis version 7.0. for bigger datasets. Mol Biol Evol 2016;33:1870-4. https://doi.org/10.1093/molbev/msw054
  4. de Cock AWAM, Lodhi AM, Rintoul TL, Bala K, Robideau GP, Gloria Abad Z, Coffey MD, Shahzad S, Levesque CA. Phytopythium: molecular phylogeny and systematics. Persoonia 2015;34:25-39. https://doi.org/10.3767/003158515X685382
  5. Bala K, Robideau GP, Levesque A, de Cock AWAM, Abad ZG, Lodhi AM, Shahzad S, Ghaffar A, Coffey MD. Phytopythium Abad, de Cock, Bala, Robideau & Levesque, gen. nov. and Phytopythium sindhum Lodhi, Shahzad & Levesque, sp. nov. Persoonia 2010;24:136-7.
  6. van der Plaats-Niterink AJ. Monograph of the genus Pythium. Stud Mycol 1981;21:1-242.
  7. Guo LY, Ko WH. Survey of root rot of Anthurium. Plant Pathol Bull 1994;3:18-23.
  8. Farr DF, Rossman AY. Fungal Databases. Systematic Mycology and Microbiology Laboratory, ARS, USDA, Retrieved December 7, 2019, from http://nt.ars-grin.gov/fungaldatabases/.