The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Crown and Root Rot of Strawberry Caused by Neopestalotiopsis clavispora in Korea

Neopestalotiopsis clavispora에 의한 딸기 뿌리썩음병 한국 내 발생

  • Park, Kyoungmi (Gyeongsangnam-do Agricultural Research and Extension Services) ;
  • Han, Inyoung (Gyeongsangnam-do Agricultural Research and Extension Services) ;
  • Lee, Seok-Min (Gyeongsangnam-do Agricultural Research and Extension Services) ;
  • Choi, Si-Lim (Gyeongsangnam-do Agricultural Research and Extension Services) ;
  • Kim, Min Chul (Division of Applied Life Science, Gyeongsang National University) ;
  • Lee, Heungsu (Gyeongsangnam-do Agricultural Research and Extension Services)
  • 박경미 (경남농업기술원 환경농업연구과) ;
  • 한인영 (경남농업기술원 환경농업연구과) ;
  • 이석민 (경남농업기술원 환경농업연구과) ;
  • 최시림 (경남농업기술원 환경농업연구과) ;
  • 김민철 (경상대학교 응용생명과학부) ;
  • 이흥수 (경남농업기술원 환경농업연구과)
  • Received : 2019.10.23
  • Accepted : 2019.12.02
  • Published : 2019.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

The occurrence of the crown and root rot on strawberry (Fragaria×ananassa Duch.) has been reported in greenhouses in Sancheong and Hamyang, Gyeongnam province, Korea in June, 2019. The infected plants showed browning rot of the inner crown and root, causing delayed development, lack of growth, and poor rooting. The browning rot of the inner crown and root can sometimes lead to wilting and collapsing of plants. Fungi were isolated from the symptomatic root and crown. Based on the results of morphological and phylogenetic analyses, the causal agent of the disease was identified to be Neopestalotiopsis clavispora. The fungal isolates were then used for inoculation into strawberry plants to determine the causal agent of the crown and root rot as per Koch's postulates. The inoculated strawberry plants showed the same symptoms as the originally infected plants, and the fungal pathogen re-isolated from the lesions showed the same morphological characteristics as the original pathogen. This is the first report on the occurrence of crown and root rot on strawberry (Fragaria×ananassa Duch.) caused by N. clavispora in Korea.

최근 경남 산청, 함양의 딸기 재배지역의 육묘하우스에서 딸기 묘목의 뿌리와 관부를 썩게 하고 지상부의 생육을 저해하며 최종적으로 식물체를 시들게 하는 뿌리썩음병(가칭)증상이 발생하였다. 이러한 병반을 나타내는 딸기 식물체 뿌리와 관부로부터 병원균을 분리하고 균학적 특성을 조사하였으며, 유전자 염기서열 분석과 계통학적 분석을 수행하였다. 그 결과, 딸기 육묘과정에서 발생한 뿌리썩음병(가칭)의 병원균은 Neopestalotiopsis clavispora로 확인되었으며, 건전한 딸기 식물체를 대상으로 접종실험을 수행한 결과 딸기에 대한 병원성을 확인하였다. 지금까지 국내에서 N. clavispora에 의한 딸기 뿌리썩음병이 보고되지 않았으므로 본 연구결과를 바탕으로 이병을 딸기뿌리썩음병으로 국내 최초로 보고한다.

Keywords

References

  1. Azodanlou R, Darbellay C, Luisier J, Villettaz J, Amado R. Quality assessment of strawberries (Fragaria species). J Agric Food Chem 2003;51:715-21. https://doi.org/10.1021/jf0200467
  2. Francisco AR, Blanco PR, Munoz BJ, Caballero LJ. The strawberry plant defense mechanism: a molecular review. Plant Cell Physiol 2011;52:1873-903. https://doi.org/10.1093/pcp/pcr136
  3. Nam HM, Kim HS, Kim TI, Lee EM. Comparion of environmental-friendly and chemical spray calendar for controlling diseases and insect pests of strawberry during nursery seasons. Res Plant Dis 2015;21:273-9. https://doi.org/10.5423/RPD.2015.21.4.273
  4. Lim YS, Jung KC, Kim SH, Bark SD. Crown rot of strawberry (Fragaria ananassa) caused by Phytophthora cactorum. Korean J Plant Pathol 1998;14: 735-7.
  5. White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninski JJ, White TJ, editors. Pcr protocols, a guide to methods and applications. San Diego: Academic Press; 1990. p. 315-22.
  6. Glass NL, Donaldson GC. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Environ Microbiol 1995;61:1323-30. https://doi.org/10.1128/aem.61.4.1323-1330.1995
  7. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Bio Evol 2013;30:2725-9. https://doi.org/10.1093/molbev/mst197
  8. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 2018;35:1547-9. https://doi.org/10.1093/molbev/msy096
  9. Yusuda F, Kobayashi T, Watanabe H, Izawa H. Addition of Pestalotiopsis spp. to leaf spot pathogens of Japanese persimmon. J Gen Plant Pathol 2003;69:29-32. https://doi.org/10.1007/s10327-002-0011-1
  10. Hopkins KE, McQuilken MP. Characteristics of Pestalotiopsis associated with hardy ornamental plants in the UK. Eur J Plant Pathol 2000;106:77-85. https://doi.org/10.1023/A:1008776611306
  11. Maharachchikumbura SSN, Hyde KD, Groenewald JZ, Xu J, Crous PW. Pestalotiopsis revisited. Stud Mycol 2014;79:121-86. https://doi.org/10.1016/j.simyco.2014.09.005
  12. Amrutha P, Vijayaraghavan R. Evaluation of fungicides and biocontrol agents against Neopestalotiopsis clavispora causing leaf blight of strawberry (Fragaria x ananassa Duch.). Int J Curr Microbiol Appl Sci 2018;7:622-8. https://doi.org/10.20546/ijcmas.2018.708.067
  13. Embaby EM. Pestalotia fruit rot on strawberry plants in Egypt. Egypt J Phytopathol 2007;35:99-110
  14. Rodrigues FA, Silva IT, Antunes Cruz MF, Carre-Missio V. The infection process of Pestalotiopsis longisetula leaf spot on strwberry leaves. J Phytopathol 2014;162:690-2. https://doi.org/10.1111/jph.12226
  15. Chamorro M, Aguado A, De los Santos B. First report of root and crown rot caused by Pestalotiopsis calvispora (Neopestalotiopsis clavispora) on strawberry in spain. Plant Dis 2016;100:1495. https://doi.org/10.1094/PDIS-11-15-1308-PDN
  16. Obregon VG, Meneguzzi NG, Ibanez JM, Lattar TE, Kirschbaum DS. First report of Neopestalotiopsis clavispora causing root and crown rot on strawberry plants in Argentina. Plant Dis 2018;102:1856.