Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Immunomodulatory Properties of Lactobacillus plantarum NC8 Expressing an Anti-CD11c Single-Chain Fv Fragment

  • Liu, Jing (College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics Key Laboratory of Animal Production and Product Quality Safety, Ministry of Education Jilin Agricultural University) ;
  • Yang, Guilian (College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics Key Laboratory of Animal Production and Product Quality Safety, Ministry of Education Jilin Agricultural University) ;
  • Gao, Xing (College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics Key Laboratory of Animal Production and Product Quality Safety, Ministry of Education Jilin Agricultural University) ;
  • Zhang, Zan (College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics Key Laboratory of Animal Production and Product Quality Safety, Ministry of Education Jilin Agricultural University) ;
  • Liu, Yang (College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics Key Laboratory of Animal Production and Product Quality Safety, Ministry of Education Jilin Agricultural University) ;
  • Yang, Xin (College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics Key Laboratory of Animal Production and Product Quality Safety, Ministry of Education Jilin Agricultural University) ;
  • Shi, Chunwei (College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics Key Laboratory of Animal Production and Product Quality Safety, Ministry of Education Jilin Agricultural University) ;
  • Liu, Qiong (College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics Key Laboratory of Animal Production and Product Quality Safety, Ministry of Education Jilin Agricultural University) ;
  • Jiang, Yanlong (College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics Key Laboratory of Animal Production and Product Quality Safety, Ministry of Education Jilin Agricultural University) ;
  • Wang, Chunfeng (College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics Key Laboratory of Animal Production and Product Quality Safety, Ministry of Education Jilin Agricultural University)
  • Received : 2018.09.12
  • Accepted : 2018.10.30
  • Published : 2019.01.28
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Abstract

The lactic acid bacteria species Lactobacillus plantarum (L. plantarum) has been used extensively for vaccine delivery. Considering to the critical role of dendritic cells in stimulating host immune response, in this study, we constructed a novel CD11c-targeting L. plantarum strain with surface-displayed variable fragments of anti-CD11c, single-chain antibody (scFv-CD11c). The newly designed L. plantarum strain, named 409-aCD11c, could adhere and invade more efficiently to bone marrow-derived DCs (BMDCs) in vitro due to the specific interaction between scFv-CD11c and CD11c located on the surface of BMDCs. After incubation with BMDCs, the 409-aCD11c strain harboring a eukaryotic vector pValac-GFP could lead to more efficient expression of GFP compared with wild-type strains shown by flow cytometry analysis, indicating the enhanced translocation of pValac-GFP from L. plantarum to BMDCs. Similar results were also observed in an in vivo study, which showed that oral administration resulted in efficient expression of GFP in both Peyer's patches (PP) and mesenteric lymph nodes (MLNs) within 7 days after the last administration. In addition, the CD11c-targeting strain significantly promoted the differentiation and maturation of DCs, the differentiation of $IL-4^+$ and $IL-17A^+$ T helper (Th) cells in MLNs, as well as production of $B220^+$ $IgA^+$ B cells in the PP. In conclusion, this study developed a novel DC-targeting L. plantarum strain which could increase the ability to deliver eukaryotic expression plasmid to host cells, indicating a promising approach for vaccine study.

Keywords

Acknowledgement

Supported by : National Natural Science Foundation of China

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