Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Single Dose Oral Toxicity Test of Water Extract of Corni Fructus in ICR Mice

ICR 마우스를 이용한 산수유 건피 추출물의 단회 경구투여 독성시험

  • Hwang-Bo, Hyun (Anti-Aging Research Center, Dong-eui University) ;
  • Kwon, Da Hye (Anti-Aging Research Center, Dong-eui University) ;
  • Kim, Min Young (Anti-Aging Research Center, Dong-eui University) ;
  • Ji, Seon Yeong (Anti-Aging Research Center, Dong-eui University) ;
  • Choi, Eun Ok (Anti-Aging Research Center, Dong-eui University) ;
  • Kim, Sung Ok (Department of Food Science and Biotechnology, College of Engineering, Kyungsung University) ;
  • Jeong, Ji-Suk (Gurye-gun Agricultural Center and Gurye Wildflower Institute) ;
  • Hong, Su Hyun (Anti-Aging Research Center, Dong-eui University) ;
  • Choi, Sung Hyun (Department of System Management, Korea Lift College) ;
  • Park, Cheol (Department of Molecular Biology, College of Natural Sciences and Human Ecology, Dong-eui University) ;
  • Choi, Yung Hyun (Anti-Aging Research Center, Dong-eui University)
  • 황보현 (동의대학교 항노화연구소) ;
  • 권다혜 (동의대학교 항노화연구소) ;
  • 김민영 (동의대학교 항노화연구소) ;
  • 지선영 (동의대학교 항노화연구소) ;
  • 최은옥 (동의대학교 항노화연구소) ;
  • 김성옥 (경성대학교 공과대학 식품응용공학부) ;
  • 정지숙 (구례야생화연구소) ;
  • 홍수현 (동의대학교 항노화연구소) ;
  • 최성현 (한국승강기대학교 승강기시스템관리과) ;
  • 박철 (동의대학교 자연과학대학 분자생물학과) ;
  • 최영현 (동의대학교 항노화연구소)
  • Received : 2018.07.11
  • Accepted : 2019.01.07
  • Published : 2019.01.30
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Abstract

Herbal medicines are widely used as therapeutic products in many countries. Corni fructus (CF), the dried ripe sarcocarp of Cornus officinalis Sieb. et Zucc (Cornaceae), has been used for thousands of years in traditional medicine and has been reported to be effective for the prevention and treatment of various diseases, such as kidney diseases and diabetes. Recent research on CF has documented a wide spectrum of therapeutic properties, which include anti-inflammatory, ant-oxidative, immunomodulatory, and anti-cancer effects. However, there is no information on its safety. Therefore, in this study, the toxicity of water extract of CF to ICR mice was investigated. The mice received a single dose of water extract of CF (1,000, 2,000, and 5,000 mg/kg of body weight) via the oral route. Mortality, clinical signs, body weight changes, gross findings, and weights of the principal organs after 14 d were then assessed. The results revealed no adverse effects of CF as determined by clinical signs, body weights, or organ weights and no gross pathological findings in any of the treatment groups. These results suggest that the 50% lethal dose and approximated lethal dose of CF extract is over 5,000 mg/kg. The findings provide scientific evidence for the safety of CFs.

산수유(Corni Fructus)는 한국을 포함한 아시아 지역에서 오랫동안 전통 의약제로 광범위하게 사용되어 왔다. 최근 연구에 따르면 산수유는 항염증, 항산화, 면역기능 조절 및 항암활성 등과 같은 많은 효능을 가지는 것으로 알려져 있다. 그러나 산수유에 대한 독성 및 안전성에 대해서는 거의 알려져 있지 않은 실정이다. 따라서 본 연구에서는 산수유 열수 추출물(CF)을 대상으로 안전성을 확인하기 위하여 단회 경구 투여에 따른 독성 시험을 실시하였다. 이를 위하여 ICR 마우스를 대상으로 1,000, 2,000 및 5,000 mg/kg의 CF를 경구 투여하였으며, 투여 후 14일 동안의 치사율, 체중 변화, 임상증상, 음수율 및 사료섭취량과 함께 부검 소견, 장기무게 변화, 혈액학 및 혈액생화학적 검사를 실시하였다. 실험 결과에 의하면 CF 투여에 따른 치사율, 임상증상, 체중 및 부검 소견 상의 유의적인 변화는 관찰되지 않았다. 또한 CF 투여에 따른 각 장기의 무게, 혈액학적 및 혈청학적 임상 화학적 지표에도 유의적인 변화는 관찰할 수 없었다. 따라서 CF 단회 경구 투여에 따른 치사량이 5,000 mg/kg 이상일 것으로 추정되어 ICR 마우스에 대하여 급성 독성이 없는 비교적 안전한 물질이라는 것을 알 수 있었으며, 향후 천연 식의약 소재로서의 효능 규명을 통한 활용이 기대된다.

Keywords

Table 1. Experimental design used in this study

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Table 2. Body weights change of ICR mice orally treated with CF for 16 days

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Table 3. Organ weights of ICR mice orally treated with CF

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Table 4. Observed necropsy finding of ICR mice orally treated with CF

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Table 5. Levels of hematological analysis in ICR mice orally treated with CF

SMGHBM_2019_v29n1_112_t0005.png 이미지

Table 6. Levels of serum biochemistry analysis in ICR mice orally treated with CF

SMGHBM_2019_v29n1_112_t0006.png 이미지

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