한국동물생명공학회지 (Journal of Animal Reproduction and Biotechnology)

  • 제34권3호
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  • Pages.232-239
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  • 2019
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  • 2671-4639(pISSN)
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  • 2671-4663(eISSN)
한국동물생명공학회 (The Korean Society of Animal Reproduction and Biotechnology)
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돼지 유도만능줄기세포 유래 복제란의 특성 분석

Developmental Characteristics of Cloned Embryos Reconstructed with Induced Pluripotent Stem Cells in Pigs

  • 권대진 (전북대학교 국제농업개발협력센터) ;
  • 오재돈 (전북대학교 동물생명과학과) ;
  • 박미령 (농촌진흥청 국립축산과학원) ;
  • 황인설 (농촌진흥청 국립축산과학원) ;
  • 박응우 (농촌진흥청 국립축산과학원) ;
  • 황성수 (농촌진흥청 국립축산과학원)
  • Kwon, Dae-Jin (International Agricultural Development and Cooperation Center, Chonbuk National University) ;
  • Oh, Jae-Don (Department of Animal Biotechnology, Chonbuk National University) ;
  • Park, Mi-Ryung (National Institute of Animal Science, Rural Development Administration) ;
  • Hwang, In-Sul (National Institute of Animal Science, Rural Development Administration) ;
  • Park, Eung Woo (National Institute of Animal Science, Rural Development Administration) ;
  • Hwang, Seongsoo (National Institute of Animal Science, Rural Development Administration)
  • 투고 : 2019.09.11
  • 심사 : 2019.09.24
  • 발행 : 2019.09.30
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초록

In general, cloned pigs have been produced using the somatic cell nuclear transfer (SCNT) technique with various types of somatic cells; however, the SCNT technique has disadvantages not only in its low efficiency but also in the development of abnormal clones. This study aimed to compare early embryonic development and quality of SCNT embryos with those of induced pluripotent stem cells (iPSCs) NT embryos (iPSC-NTs). Ear fibroblast cells were used as donor cells and iPSCs were generated from these cells by lentiviral transduction with human six factors (Oct4, Sox2, c-Myc, Nanog, Klf4 and Lin28). Blastocyst formation rate in iPSC-NT (23/258, 8.9%) was significantly lower than that in SCNT (46/175, 26.3%; p < 0.05). Total cell number in blastocysts was similar between two groups, but blastocysts in iPSC-NT had a lower number of apoptotic cells than in SCNT (2.0 ± 0.6 vs. 9.8 ± 2.9, p < 0.05). Quantitative PCR data showed that apoptosis-related genes (bax, caspase-3, and caspase-9) were highly expressed in SCNT than iPSC-NT (p < 0.05). Although an early development rate was low in iPSC-NT, the quality of cloned embryos from porcine iPSC was higher than that of embryos from somatic cells. Therefore, porcine iPSCs could be used as a preferable cell source to create a clone or transgenic animals by using the NT technique.

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