한국동물생명공학회지 (Journal of Animal Reproduction and Biotechnology)

  • 제34권4호
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  • Pages.300-304
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  • 2019
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  • 2671-4639(pISSN)
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  • 2671-4663(eISSN)
한국동물생명공학회 (The Korean Society of Animal Reproduction and Biotechnology)
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Recovery Efficiency of Cumulus Oocyte Complexes (COCs) according to Collection Frequency for Ovum Pick-up (OPU) Method in Hanwoo Cow

  • 투고 : 2019.12.02
  • 심사 : 2019.12.06
  • 발행 : 2019.12.31
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초록

This study was conducted to examine the oocyte recovery efficiency through having an OPU session once and twice a week. Also, the oocyte recovery efficiency was examined by using OPU after two and three months of rest period. Six cows were used for oocytes collection and were randomly divided into two groups. In experiment 1, OPU sessions were conducted once and twice a week to collect oocytes. The collected oocytes between once and twice OPU groups were classified into four groups (grade 1, 2, 3 and 4) according to the quality of cumulus cells and ooplasm. Based on the result, the percentage of collected oocytes per aspirated follicle number was similar between once and twice OPU session groups (65.5 ± 1.9 and 68.7 ± 1.4 vs.). However, the percentage of grade 1 oocytes from the twice OPU session group was significantly high compared with that of the once a week OPU session group (25.3 ± 0.9 and 32.5 ± 1.2% vs. once and twice session group, respectively, p < 0.05). In experiment 2, the group with three months of rest period tended to have a high percentage of collected oocyte compared with the group with two months of rest period (64.6 and 70.9% vs. 2 and 3 months rest group, respectively, p = 0.62). The percentage of grade 4 in the group with three months of rest period was significantly low compared with the group with two months of rest period group (27.3 and 36.5% vs. two and three months rest group, respectively, p = 0.05). In conclusion, twice a week OPU session is suitable for collection of high quality oocytes by using OPU, and three months of rest period is needed for the recovery of oocyte quality of a donor cow.

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참고문헌

  1. Bols, P. E. J., Leroy, J. L. M. R., Vanholder, T. & Van Soom, A. 2004. A comparison of a mechanical sector and a linear array transducer for ultrasound-guided transvaginal oocyte retrieval (OPU) in the cow. Theriogenology, 62:906-914. https://doi.org/10.1016/j.theriogenology.2003.12.016
  2. Chaubal, S. A., Molina, J. A., Ohlrichs, C. L., Ferre, L. B., Faber, D. C., Bols, P. E. J., Riesen, J. W., Tian, X. & Yang, X. 2006. Comparison of different transvaginal ovum pick-up protocols to optimise oocyte retrieval and embryo production over a 10-week period in cows. Theriogenology, 65:1631-1648. https://doi.org/10.1016/j.theriogenology.2005.07.020
  3. Galli, C., Crotti, G., Notari, C., Turini, P., Duchi, R. & Lazzari, G. 2001. Embryo production by ovum pick up from live donors. Theriogenology, 55:1341-1357. https://doi.org/10.1016/S0093-691X(01)00486-1
  4. Hashimoto, S., Takakura, R., Minami, N. & Yamada, M. 1999. Ultrasound-guided follicle aspiration: Effect of the frequency of a linear transvaginal probe on the collection of bovine oocytes. Theriogenology, 52:131-138. https://doi.org/10.1016/S0093-691X(99)00115-6
  5. Jin, J. I., Ghanem, N., Kim, S. S., Choi, B. H., Ha, A. N., Lee, K. L., Sun, D. W., Lim, H. T., Lee, J. G. & Kong, I. K. 2016. Interaction of donor age, parity and repeated recovery of cumulus-oocyte complexes by ovum pick-up on in vitro embryo production and viability after transfer. Livestock Science, 188:43-47. https://doi.org/10.1016/j.livsci.2016.04.002
  6. Konrad, J., Clerico, G., Garrido, M. J., Taminell, G., Yuponi, M., Yuponi, R., Crudeli, G. & Sansinena, M. 2017. Ovum pick-up interval in buffalo (Bubalus bubalis) managed under wetland conditions in Argentina: Effect on follicular population, oocyte recovery, and in vitro embryo development. Animal Reproduction Science, 183:39-45. https://doi.org/10.1016/j.anireprosci.2017.06.004
  7. Kruip, T. A. M., Boni, R., Wurth, Y. A., Roelofsen, M. W. M. & Pieterse, M. C. 1994. Potential Use of Ovum Pick-up for Embryo Production and Breeding in Cattle. Theriogenology, 42:675-684. https://doi.org/10.1016/0093-691X(94)90384-U
  8. Neglia, G., Gasparrini, B., Vecchio, D., Boccia, L., Varricchio, E., Di Palo, R., Zicarelli, L. & Campanile, G. 2011. Long term effect of Ovum Pick-up in buffalo species. Animal Reproduction Science, 123:180-186. https://doi.org/10.1016/j.anireprosci.2011.01.011
  9. Pontes, J. H. F., Sterza, F. A. M., Basso, A. C., Ferreira, C. R., Sanches, B. V., Rubin, K. C. P. & Seneda, M. M. 2011. Ovum pick up, in vitro embryo production, and pregnancy rates from a large-scale commercial program using Nelore cattle (Bos indicus) donors. Theriogenology, 75:1640-1646. https://doi.org/10.1016/j.theriogenology.2010.12.026
  10. Seneda, M. M., Esper, C. R., Garcia, J. M., Andrade, E. R., Binelli, M., Oliveira, J. A. & Nascimento, A. B. 2003. Efficacy of linear and convex transducers for ultrasound-guided transvaginal follicle aspiration. Theriogenology, 59:1435-1440. https://doi.org/10.1016/S0093-691X(02)01188-3
  11. Stubbings, R. B. & Walton, J. S. 1995. Effect of Ultrasonically-Guided Follicle Aspiration on Estrous-Cycle and Follicular Dynamics in Holstein Cows. Theriogenology, 43:705-712. https://doi.org/10.1016/0093-691X(95)00013-X
  12. Ward, F. A., Lonergan, P., Enright, B. P. & Boland, M. P. 2000. Factors affecting recovery and quality of oocytes for bovine embryo production in vitro using ovum pick-up technology. Theriogenology, 54:433-446. https://doi.org/10.1016/S0093-691X(00)00360-5
  13. Yang, X. Y., Zhao, J. G., Li, H., Liu, H. F., Huang, Y., Huang, S. Z., Zeng, F. Y. & Zeng, Y. T. 2008. Effect of individual heifer oocyte donors on cloned embryo development in vitro. Animal Reproduction Science, 104:28-37. https://doi.org/10.1016/j.anireprosci.2007.01.017