대한의생명과학회지 (Biomedical Science Letters)

  • 제25권4호
  • /
  • Pages.313-320
  • /
  • 2019
  • /
  • 1738-3226(pISSN)
  • /
  • 2288-7415(eISSN)
대한의생명과학회 (The Korean Society for Biomedical Laboratory Sciences)
권호 표지
DOI QR코드

DOI QR Code

Phytochemical Analysis and Wound Healing Potential of Ethanol Extract of Sea Mustard and Sea Mustard Sporophyll

  • Kim, Jin (Department of Oral and Maxillofacial Surgery, College of Dentistry, Chosun University & Institute of Dental Science, Chosun University) ;
  • Lee, Chang-Moon (Department of Biomedical Engineering, Chonnam National University) ;
  • Kim, Su-Gwan (Department of Oral and Maxillofacial Surgery, College of Dentistry, Chosun University & Institute of Dental Science, Chosun University)
  • 투고 : 2019.07.01
  • 심사 : 2019.12.05
  • 발행 : 2019.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

In this study, phytochemicals extracted from sea mustard (SM) and sea mustard sporophyll (SMS) in ethanol solution have been analyzed and wound healing potential of the phytochemicals was investigated. In the phytochemical screening studies, the extract of SM and SMS includes several phytochemical compounds such as phytol, ascorbic acid, sitgmasta, fucosterol and ergosta. Cytotoxicity studies of the extract of SM and SMS with mouse macrophage RAW 264.7 cells showed on toxicity up to a high concentration of 1.0 mg/mL. Furthermore, the SM and SMS extract significantly reduces the production of nitric oxide (NO) induced lipopolysaccharide on RAW 264.7 cells with a dose-dependent manner. In addition, the extract of SM and SMS has the effect of enhancing the cell migration and invasion of fibroblast. These results demonstrate that the extract of SM and SMS could help to heal wound by reducing NO production and increasing cell migration.

키워드

참고문헌

  1. Ahn SM, Hong YK, Kwon GS, Sohn HY. Evaluation on in-vitro anticoagulation activity of 35 different seaweed extracts. Journal of Life Science. 2010. 20: 1640-1647. https://doi.org/10.5352/JLS.2010.20.11.1640
  2. Alfadda AA, Sallam RM. Reactive oxygen species in health and disease. Journal of Biomedicine and Biotechnology. 2012. 936486.
  3. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature. 1958. 181: 1199-1200. https://doi.org/10.1038/1811199a0
  4. Burcova Z, Kreps F, Greifova M, Jablonsky M, Haz A, Schmidt S, Surina I. Antibacterial and antifungal activity of phytosterols and methyl dehydroabietate of Norway spruce bark extracts. Journal of Biotechnology. 2018. 20: 18-24.
  5. Chung JH, Youn SH, Kwon OS, Cho KH, Youn JI, Eun HC. Regulations of collagen synthesis by ascorbic acid, transforming growth factor-beta and interferon-gamma in human dermal fibroblasts cultured in three-dimensional collagen gel are photoaging and aging independent. Journal of Dermatological Science. 1997. 15: 188-200. https://doi.org/10.1016/S0923-1811(97)00607-5
  6. Dumas M, Chaudagne C, Bonte F, Meybeck A. Age-related response of human dermal fibroblasts to 1-ascorbic acid. study of type I and III collagen synthesis. Comptes Rendus de l'Academie des Sciences - Series III - Sciences de la Vie. 1996. 319: 1127-1132.
  7. Guaratini T, Lopes NP, Marinho-Soriano E, Colepicolo P, Pinto E. Antioxidant activity and chemical composition of the non polar fraction of Gracilaria domingensis (Kutzing) Sonder ex Dickie and Gracilaria birdiae. Brazilian Journal of Pharmacy. 2012. 22: 724-729. https://doi.org/10.1590/S0102-695X2012005000063
  8. Gupta S, Abu-Ghanna N. Bioactive potential and possible health effects of edible brown seaweeds. Trends in Food Science & Technology. 2011. 6: 315-326. https://doi.org/10.1016/j.tifs.2011.03.011
  9. Jung HA, Jin SE, Ahn BR, Lee CM, Choi JS. Anti-inflammatory activity of edible brown alga Eisenia bicyclis and its constituents fucosterol and phlorotannins in LPS-stimulated RAW 264.7 macrophages. Food and Chemical Toxicology. 2013. 59: 199-206. https://doi.org/10.1016/j.fct.2013.05.061
  10. Khurana S, Piche M, Hollingsworth A, Venkataraman K, Tai TC. Oxidative stress and cardiovascular health: Therapeutic potential of polyphenols. Can. The Korean Journal of Physiology & Pharmacology. 2013. 91: 198-212. https://doi.org/10.1139/cjpp-2012-0252
  11. Kim J, Lee CM. Anti-inflammatory effects and influence on fibroblast growth of astaxanthin-cyclodextrin nanoparticles. Journal of Chitin and Chitosan. 2018. 23: 170-175. https://doi.org/10.17642/jcc.23.3.5
  12. Kim JW, Kwon YR, Youn KS. Quality characteristics and antioxidant properties in spray-dried and freeze-dried powder prepared with powdered seaweed extracts. Korean Society of Food Science and Technology. 2012. 44: 716-721. https://doi.org/10.9721/KJFST.2012.44.6.716
  13. Kim JS, Bak EJ, Lee BC, Kim YS, Park JB, Choi IG. Neuregulin induces HaCaT keratinocyte migration via Rac1-mediated NADPH-oxidase activation. Journal of Cellular Physiology. 2011. 226: 3014-3021. https://doi.org/10.1002/jcp.22649
  14. Kim JS. Effect of rudbeckia laciniata extract on physiological activity of HaCaT Cells, The Korean Journal of Food and Nutrition. 2016. 29: 335-340. https://doi.org/10.9799/ksfan.2016.29.3.335
  15. Kim HS, Lee JH, Yeon CJ, Lee JS. The therapeutic effect of porcine placenta extract for improvement sequelae of burn. Journal of Korean Burn Society. 2012. 15: 96-101.
  16. Lee AR, Roh SS, Kim HK. Anti-microbial activity and antiinflammatory effects of fucoidan extracts. Asian Journal of Beauty and Cosmetology. 2018. 16: 191-200. https://doi.org/10.20402/ajbc.2017.0171
  17. Lee YC, Hwang KH, Han DH, Kim SD. Compositions of opuntia ficus-indica. The Journal of Food Science and Technology. 1997. 29: 847-853.
  18. Moncada S, Palmer RMJ, Higgs EA. Nitric oxide: physiology, pathology, and pharmacology. Pharmacological Reviews. 1991. 43: 109-142.
  19. Okoli CO, Akah PA, Nwafor SV, Anisiobi AI, Ibegbunam IN, Erojikwe O. Anti-inflammatory activity of hexane leaf extract of Aspilia africana C.D. Adams. Journal of Ethnopharmacology. 2007. 109: 219-225 https://doi.org/10.1016/j.jep.2006.07.037
  20. Pasparakis M, Haase I, Nestle FO. Mechanisms regulating skin immunity and inflammation. Nature Reviews Immunology. 2014. 14: 289-301. https://doi.org/10.1038/nri3646
  21. Noda H, Amano H, Arashima K, Hashimoto S, Nisizawa K. Studies on the antitumour activity of marine algae. Bulletin of the Japanese Society of Scientific Fisheries. 1989. 55: 1259-1264. https://doi.org/10.2331/suisan.55.1259
  22. Song YS, Balcos MC, Yun HY, Baek KJ, Kwon NS, Kim MK, Kim DS. ERK activation by fucoidan leads to inhibition of melanogenesis in Mel-Ab cells. The Korean Journal of Physiology and Pharmacology. 2015. 19: 29-34. https://doi.org/10.4196/kjpp.2015.19.1.29
  23. Stierle AA, Stierle DB, Kelly K. Berkelic acid, a novel spiroketal with selective anticancer activity from an acid mine waste fungal extremophile. The Journal of Organic Chemistry. 2006. 71: 5357-5360. https://doi.org/10.1021/jo060018d
  24. Surh YJ, Chun KS, Cha HH, Han SS, Keum YS, Park KK, Lee SS. Molecular mechanisms underlying chemopreventive activities of anti-inflammatory phytochemicals: down-regulation of COX-2 and iNOS through suppression of $NF{\kappa}B$ activation. Mutation Research. 2001. 480: 243-268.
  25. Rousselle P, Braye F, Dayan G. Re-epithelialization of adult skin wounds: cellular mechanisms and therapeutic strategies. Advanced Drug Delivery Reviews, 2018.
  26. Torres FAE, Passalacqua TG, Velasquez AMA, Souza RA, Colepicolo P, Graminha, MAS. New drugs with antiprotozoal activity from marine algae: a review. Brazilian Journal of Pharmacy. 2014. 24: 265-276. https://doi.org/10.1016/j.bjp.2014.07.001
  27. Wiegand C, Abel M, Hipler UC, Elsner P, Effect of non-adhering dressings on promotion of fibroblast proliferation and wound healing in vitro. Scientific Reports. 2019. 9: 4320-4330. https://doi.org/10.1038/s41598-019-40921-y
  28. Zang Q, Maass DL, White J, Horton JW. Cardiac mitochondrial damage and loss of ROS defense after burn injury: the beneficial effect of antioxidnat therapy. Journal of Applied Physiology. 2007. 102: 103-112. https://doi.org/10.1152/japplphysiol.00359.2006