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In Vitro Propagation of Commonly Used Medicinal Trees in Korea

  • An, Chanhoon (Forest Medicinal Resources Research Center, National Institute of Forest Science)
  • Received : 2019.09.06
  • Accepted : 2019.10.24
  • Published : 2019.12.31

Abstract

Forest medicinal resources, which constitute one of the non-timber forest products, have been regarded as healthy and highly valued products. To meet the increasing demand of the medicinal resources, it is necessary to improve the propagation methods of medicinal plants. In vitro propagation not only allows an opportunity for propagating plants in large numbers but also allows for enhancing the quality and quantity of the desired functional component of a plant by altering the growth factors, such as medium, carbon source, and plant growth regulators influence plant. There have been several studies of in vitro propagation methods, such as axillary bud culture, shooting, and embryogenesis, on Kalopanax septemlobus, Eleutherococcus sessiliflorus, Hovenia dulcis, and Schisandra chinensis in Korea between from 2000 through 2010. Furthermore, there have been attempts to proliferate callus and plantlets for producing useful natural compounds by using bioreactors. Here, we provide an account of the in vitro propagation methods of medicinal trees in Korea based on a review of several micropropagation studies.

Keywords

References

  1. Bae KH, Kim JA, Choi YE. 2009. Induction and in vitro proliferation of adventitious roots in Dendropanax morbifera. J Plant Biotechnol 36: 163-169. https://doi.org/10.5010/JPB.2009.36.2.163
  2. Beruto M, Lanteri L, Portogallo C. 2004. Micropropagation of tree peony (Paeonia suffruticosa). Plant Cell Tissue Organ Cult 79: 249-255. https://doi.org/10.1007/s11240-004-0666-8
  3. Bouza L, Jacques M, Sotta B, Miginiac E. 1994. Relatiopns between auxin and cytokinin contents and in vitro rooting of tree peony (Paeonia suffruticosa Andr.). Plant Growth Regul 15: 69-73. https://doi.org/10.1007/BF00024678
  4. Chen AH, Yang JL, Niu YD, Yang CP, Liu GF, Yu CY, Li CH. 2010. High-frequency somatic embryogenesis from germinated zygotic embryos of Schisandra chinensis and evaluation of the effects of medium strength, sucrose, GA3, and BA on somatic embryo development. Plant Cell Tiss Organ Cult 102: 357-364. https://doi.org/10.1007/s11240-010-9740-6
  5. Chen R, Namimatsu S, Nakadozono Y, Bamba T, Nakazawa Y, Gyokusen K. 2008. Efficient regeneration of Eucommia ulmoides from hypocotyl explant. Biol Plant 52: 713-717. https://doi.org/10.1007/s10535-008-0137-x
  6. Cheong EJ, Moon HK. 1999. Light, cytokinins and explant's position on adventitious bud induction from the leaf segments of Populus euphratica Olive. J For Sci 62: 92-98.
  7. Cheong EJ. 2018. Propagation of Three Rubus Species Native to Korea by Stem Cutting. J For Environ Sci 34: 173-175.
  8. Cheong EJ. 2019. Organogenesis from Callus Derived from In Vitro Root Tissues of Wild Prunus yedoensis Matsumura. J For Environ Sci 35: 41-46.
  9. Choi MS, Kwak SS, Liu JR, Lee IK, Yoo ID. 2001. Production of novel flavonoids in cell cultures of Cudrania tricuspidata. J Korean Plant Tiss Cult 28: 159-164.
  10. Choi SK, Yun KW. 2001. In vitro propagation using shoot tip culture in Gold Tree (Dendropanax morbifera Lev.). Kor J Crop Sci 46:464-467.
  11. Choi YE, Ko SK, Lee KS, Yoon ES. 2002. Production of plantlets of Eleutherococcus sessiliflorus via somatic embryogenesis and successful transfer to soil. Plant Cell Tiss Organ Cult 69: 201-204. https://doi.org/10.1023/A:1015290718273
  12. Choi YE, Yeo UD, Soh WY. 1986. Somatic embryogenesis and plantlet regeneration from stem callus of Cedrela sinensis. Korean J Plant Tiss Cult 13: 61-70.
  13. Clark CA, Hoy MW. 2006. Effects of Common Viruses on Yield and Quality of Beauregard Sweetpotato in Louisiana. Plant Dis 90: 83-88. https://doi.org/10.1094/PD-90-0083
  14. Conci VC, Canavelli A, Lunello P, Di Rienzo J, Nome SF, Zumelzu G, Italia R. 2003. Yield Losses Associated with Virus-Infected Garlic Plants During Five Successive Years. Plant Dis 87: 1411-1415. https://doi.org/10.1094/PDIS.2003.87.12.1411
  15. Doo HS, Li HL, Kwon TH, Yang MS. 2000. Development of Aspceptic Seedling by In Vitro Germination in Lacquer Tree Seed. Kor J Plant Res 13: 48-53.
  16. Echeverrigaray S, Mossi AJ, Munari F. 1998. Micropropagation of Raisin Tree (Hovenia dulcis Thunb.) Through Axillary Bud Culture. J Biochem Biotechnol 7: 99-102.
  17. Eom SH, Shin DY, Lee HY, Kim MJ, Kim JD, Choi WC, Heo K, Yu CY. 2002. Somatic embryogenesis and plant regeneration of Hovenia dulcis Thunb. Korean J Medicinal Crop Sci 10: 41-45.
  18. Fisher JR. 2012. First Report of Tobacco rattle virus Associated with Ring Spot and Line Pattern Disease of Peony in Ohio. Plant Health Progress 13: 40. https://doi.org/10.1094/PHP-2012-0711-01-BR
  19. Hwang SJ, Hwang B. 2003. An Efficient in vitro propagation of Zanthoxylum piperitum DC. Korean J Medicinal Crop Sci 11: 316-320.
  20. Hyun YI, Moon HK, Lee SK. 1991. In vitro of 4 plus tree clones of Quercus acutissima. Res Rep Inst For Gen Korea 27: 69-74.
  21. Jang WW, Park JS, Rubenecia MRU, Park CB, Ahn YS, Lee SC. 2013. Seed production and distribution system improvement of medicinal crop seeds. Curr Res Agric Life Sci 31: 286-294. https://doi.org/10.14518/crals.2013.31.4.008
  22. Jeong MJ, Song HJ, Park DJ, Min JY, Jo JS, Kim BM, Kim HG, Kim YD, Kim RM, Karigar CS, Choi MS. 2009. High frequency plant regeneration following abnormal shoot organogenesis in the medicinal tree Hovenia dulcis. Plant Cell Tissue Organ Cult 98: 59-65. https://doi.org/10.1007/s11240-009-9538-6
  23. Jin TE. 2015. Biological resources, Core material of bio-economic period. Sci Technol Policy 25: 20-25. (in Korean)
  24. Jo MH, Ham IK, Lee BC, Kim JW, Lee WS, Kwon SY, Lee HS, Kwak SS. 2004. High frequency shoot formation and plant regeneration from cotyledonary hypocotyl explants of boxthorn (Lycium chinense Mill.) seedling. Kor J Plant Res 31: 203-207.
  25. Kim DC, Chung HJ, Min BH, Yang DC. 2002. Plant regeneration from explant types and cultivars of boxthorn (Lycium chinense Mill.) Kor J Plant Res 29: 15-18.
  26. Kim HJ, Kim WB, Yoo DL, Kim SJ, Lee JG. 2008. Proliferation of Embryogenic Callus of Kalopanax pictus through Suspension Culture System. Korean J Plant Res 21: 60-65.
  27. Kim HT, Kim ST, Lee WY, Park EJ. 2013. Induction and Growth of Vegetative Stems through In Vitro Culture of Gastrodia elata. Korean J Medicinal Crop Sci 21: 142-147. https://doi.org/10.7783/KJMCS.2013.21.2.142
  28. Kim KJ. Kim BW, Roh KS. 1996. Artifical seed production from somatic embryos and its germination in Lycium chinense Mill. Kor J Plant Res 28: 255-261.
  29. Kim MH. 2017. The study for the production of Korean medical crops and the export-import movement and the improving methods of them. Korean J Herbol 32: 1-16. https://doi.org/10.6116/kjh.2017.32.1.1.
  30. Kim SJ, Dewir YH, Moon HK. 2011. Large-scale plantlets conversion from cotyledonary somatic embryos of Kalopanax septemlobus tree using bioreactor cultures. J Plant Biochem Biotechnol 20: 241-248. https://doi.org/10.1007/s13562-011-0052-7
  31. Kim SJ, Moon HK. 2009. Establishment of suspension culture condition for embryogenic callus proliferation and somatic embryo development of Kalopanax septemlobus. J Plant Biotechnol 36: 7-12. https://doi.org/10.5010/JPB.2009.36.1.007
  32. Kim YW, Choi YE, Yi JS, Moon HK. 2007. Germination of Artificial Seeds by Encapsulation of Somatic Embryos of Kalopanax septemlobus with Alginic Acid. J Plant Biotechnol 34: 229-235. https://doi.org/10.5010/JPB.2007.34.3.229
  33. Lee CH, Min JY, Jung HN, Kim KS, Choi MS. 2007. Rapid Propagation through Tissue Culture of Cudrania tricuspidata, Medicinal Plant. Korean J Medicinal Crop Sci 15: 315-318.
  34. Lee HS, Yi JS, An CH, Lee JH. 2015. Analysis of Research on Non-Timber Forest Plants - Based on the Articles Published in the Journal of Korean Forest Society from 1962 to 2013 -. J Korean For Soc 104: 337-351. https://doi.org/10.14578/jkfs.2015.104.3.337
  35. Lee JD, Cho DY, Soh WY. 2002. Somatic embryogenesis and adventitious bud formation from zygotic embryo of boxthone (Lycium chinense Mill.). Kor J Plant Biotechnol 29: 241-246. https://doi.org/10.5010/JPB.2002.29.4.241
  36. Lee JH, Lee SY. 1997. Factors affecting anther culture in Schizandra chinensis. Korean J Medicinal Crop Sci 5: 14-20.
  37. Lee JS, Kwon KW, Bae CH, Yang DC. 2001. Advenced regeneration and genetic transformation of Lycium chinense harboring salt tolerance genes. Kor J Plant Tiss Cult 28: 47-52.
  38. Lee NN, Choi YE, Moon HK. 2015. Somatic embryo induction and plant regeneration from cold-stored embryogenic callus of K. septemlobus. J Plant Biotechnol 42: 388-395. https://doi.org/10.5010/JPB.2015.42.4.388
  39. Li CH, Kim NY, Kim MJ, Cho DH, Lee DW, Lee JG, Lim JD, Yu CY. 2006. Somatic Embryogenesis and Plant Regeneration in Embryogenic Cell Suspension Cultures of Hovenia dulcis Thunb. Korean J Medicinal Crop Sci 14: 255-260.
  40. Lim HT, Kim JH, Yeonung YR, Song YN. 1993. Establishment of mass propagation system of Zanthoxylum piperitum DC. through in vitro culture. J Agri Sci 5: 83-91.
  41. Moon HK, Kim SH, Kim BK. 2002. Micropropagation of Kalopanax pictus Nakai via axillary bud cultures. J Kor For Soc 91: 775-780.
  42. Moon HK, Lee HS, Paek KY, Park SY. 2015. Osmotic stress and strong 2,4-D shock stimulate somatic-to-embryogenic transition in Kalopanax septemlobus (Thunb.) Koidz. Acta Physiol Plant 37: 1710. https://doi.org/10.1007/s11738-014-1710-x
  43. Moon HK, Park SY, Kim YW, Kim SH. 2008. Somatic embryogenesis and plantlet production using rejuvenated tissues from serial grafting of a mature Kalopanax septemlobus tree. Vitro Cell Dev Biol Plant 44: 119-127. https://doi.org/10.1007/s11627-008-9122-5
  44. Moon HK, Youn Y. 1996. Somatic embryogenesis and plant regeneration from cultured immature zygotic embryos of Tilia mandshurica Rupr. Res Rep For Gen Res Inst Korea 32: 64-71.
  45. Paek KY, Yu KJ, Park SI. 1998. Comparison of Growth Characteristics and Virus Infection between Tissue - cultured Plants and Conventionally Propagated Flants of Rehmannia glutinosa LIBOSCHITZ. Korean J Medicinal Crop Sci 6: 62-69.
  46. Park CH, Seong NS, Lee ST, Youn KB, Son SG. 1993. Studies on multiplication of Cornus officinalis by in vitro culture I. Callus Induction, shoot propagation and root differentiation through bud culture. Korean J Medicinal Crop Sci 1: 63-69.
  47. Park DJ, Kang YM, Jung HN, Min JY, Kim YD, Karigar CS, Choi MS. 2006. Rapid Micropropagation of Hovenia dulcis Thunb. Through in vitro Stem Nodal Cultures. J Korean For Soc 95: 155-159.
  48. Park MY, Wang FB, Eom SH, Lee SW. 2012. In vitro Shoot Propagation Derived from Stem and Shoot Tip in Hovenia dulcis var. koreana Nakai by Plnat Growth Regulators and Light Resources. Korean J Medical Crop Sci 20: 47-53. https://doi.org/10.7783/KJMCS.2012.20.1.047
  49. Park SY, Moon HK, Kim YW. 2011. The Photoautotrophic Culture System Promotes Photosynthesis and Growth of Somatic Embryo-derived Plantlets of Kalopanax septemlobus. J Korean Soc For Sci 100: 212-217.
  50. Ribeiro IG, Gayer CRM, Castro TC, Coelho MGP, Albarello N. 2015. Compact callus cultures and evaluation of the antioxidant activity of Hovenia dulcis Thunb. (Rhamnaceae) under in vivo and in vitro culture conditions. J Medicinal Plants Res 9: 8-15. https://doi.org/10.5897/JMPR2014.5622
  51. Seo YR, Kim YD, Park DJ, Song HJ, Kim HG, Yang WH, Jeong MJ, Im HJ, Choi MS. 2016. Micropropagation of Acer tegmentosum Max. through induction of multiple shoots from axillary bud cultures. J Agric Life Sci 50: 23-32. https://doi.org/10.14397/jals.2016.50.1.23
  52. Shohael AM, Ali MB. Hahn EJ. Paek KY. 2007. Glutathione metabolism and antiocidant responses during Eleutherococcus senticosus somatic embryo development in a bioreactor. Plant Cell Tissue Organ Cult 89: 121-129. https://doi.org/10.1007/s11240-007-9220-9
  53. Shohael AM, Ali MB, Yu KW, Hahn EJ, Paek KY. 2006a. Effect of light on oxidative stress, secondary metabolites and induction of antioxidant enzymes in Eleutherococcus senticosus somatic embryos in bioreactor. Process Biochem 41: 1179-1185. https://doi.org/10.1016/j.procbio.2005.12.015
  54. Shohael AM, Ali MB, Yu KW, Hahn EJ, Paek KY. 2006b. Effect of temperature on secondary metabolites production and antioxidant enzyme activities in Eleutherococcus senticosus somatic embryos. Plant Cell Tissue Organ Cult 85: 219-228. https://doi.org/10.1007/s11240-005-9075-x
  55. Shohael AM, Chakrabarty D, Yu KW, Hahn EJ, Paek KY. 2005. Application of bioreactor system for large-scale production of Eleutherococcus sessiliflorus somatic embryos in an air-lift bioreactor and production of eleutherosides. J Biotechnol 120: 228-236. https://doi.org/10.1016/j.jbiotec.2005.06.010
  56. Shohael AM, Khatun SM, Alam MF, Paek KY. 2013. Effects of Murashige and Skoog medium strength on germination and secondary metabolites production of Eleutherococcus senticosus's somatic embryos in bioreactor. Int J Biosci 3: 155-163. https://doi.org/10.12692/ijb/3.3.155-163
  57. Shung Q. 2010. Studies on tissue culture and rapid propagation of Sorbus commixta Hedl. North Hortic 8: 167-169.
  58. Smiskova A, Vlasinova H, Havel L. 2005. Somatic embryogenesis from zygotic embryos of Schisandra chinensis. Biol Plant 49: 451-454. https://doi.org/10.1007/s10535-005-0027-4
  59. Smith CC, Jernstedt JA. 1989. In vitro development of adventitious shoots in Euonymus alatus (Celastraceae). Sci Hortic 41: 161-169. https://doi.org/10.1016/0304-4238(89)90060-5
  60. Soh WY, Yeo UD, So SS. 1990. Artificial seed production and somatic embryogenesis from the cultured cells of Cedrela sinensis Juss. Korea J Plant Tiss Cult 17: 231-238.
  61. Song WS, Chi HJ. 1995. In vitro propagation of Zanthoxylum piperitum DC. -II. Effect of $NH_4NO_3,\;KNO_3$ and casein hydrolysate on somatic embryogenesis-. J Oriental Bot Res 8: 153-157.
  62. Song WS, Oh SD, Park EH, Yu SO. 1991. In vitro propagation of Zanthoxylum piperitum DC. I. Somatic embryogenesis and plant regeneration. Korean J Plant Tiss Cult 18: 17-25.
  63. Sun D, Lang WX, Li HB, Guo XW, Lian ML, Piao ZY. 2013. An Improve Method for Somatic Embryogenesis of Schisandra chinensis (Turcz.) Baillon. Pak J Biol Sci 16: 127-134. https://doi.org/10.3923/pjbs.2013.127.134
  64. Thammina C, He M, Lu L, Cao K, Yu H, Chen Y, Tian L, Chen J, McAvoy R, Ellis D, Zhao D, Wang Y, Zhang X, Li Y. 2011. In vitro regeneration of triploid plants of Euonymus alatus 'Compactus' (Burning Bush) from endosperm tissue. HortScience 46: 1141-1147. https://doi.org/10.21273/HORTSCI.46.8.1141
  65. Wang H. HE S, Tanaka M, Van PT, Silva JAT. 2010. Effects of 2,4-D on callus formation in tree peony (Paeonia suffruticosa Andr.) under different light conditions. Floriculture Ornamental Biotech 4: 99-102.
  66. Wang H, Staden JV. 2001. Establishment of in vitro cultures of tree peonies. S Afr J Bot 67: 358-361. https://doi.org/10.1016/S0254-6299(15)31141-8
  67. Xue JP, Zhang AM, Wang YH, Sheng W. 2003. Study on plant tissue culture of Cornus officinalis. China J Chin Mater Medica 28: 118-121. https://doi.org/10.3321/j.issn:1001-5302.2003.02.006
  68. Yang J, Zhou C, Liu L, Jia D, Yin Z, Kim M, Yu C, Li C. 2012. High conversion frequency of germinated somatic embryos of Siberian ginseng (Eleutherococcus senticosus Maxim) using a bubble column bioreactor. Plant Cell Tissue Organ Cult 110: 289-298. https://doi.org/10.1007/s11240-012-0150-9
  69. Yoon ES, Kwon HK, Cho YY. 2006. Correlation between in vitro plant regeneration and polarity with Boxthorn (Lycium chinense Mill.) seedlings. Kor J Plant Res 19: 308-314.
  70. Yoo RH, Zhao F, Lim S, Igori D, Kim SM, An TJ, Lee SH, Moon JS. 2015. The complete genome sequences of two isolates of cnidium vein yellowing virus, a tentative new member of the family Secoviridae. Arch Virol 160: 2911-2914. https://doi.org/10.1007/s00705-015-2557-1