DOI QR코드

DOI QR Code

The study of blood transcriptome profiles in Holstein cows with miscarriage during peri-implantation

  • Zhao, Guoli (Department of Animal Husbandry, Agricultural College of Ningxia University) ;
  • Li, Yanyan (Helan Mountain Diary Company of Ningxia) ;
  • Kang, Xiaolong (Department of Animal Husbandry, Agricultural College of Ningxia University) ;
  • Huang, Liang (Helan Mountain Diary Company of Ningxia) ;
  • Li, Peng (Department of Animal Husbandry, Agricultural College of Ningxia University) ;
  • Zhou, Jinghang (Department of Animal Husbandry, Agricultural College of Ningxia University) ;
  • Shi, Yuangang (Department of Animal Husbandry, Agricultural College of Ningxia University)
  • Received : 2017.10.24
  • Accepted : 2018.05.24
  • Published : 2019.01.01

Abstract

Objective: In this study, the transcriptome profile of cow experiencing miscarriage during peri-implantation was investigated. Methods: Total transcriptomes were checked by RNA sequencing, and the analyzed by bioinformatics methods, the differentially expressed genes (DEGs) were analysed with hierarchical clustering and Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis. Results: The results suggested that serum progesterone levels were significantly decreased in cows that miscarried as compared to the pregnant cows at 18, 21, 33, 39, and 51 days after artificial insemination. The RNA sequencing results suggested that 32, 176, 5, 10, and 2 DEGs were identified in the pregnant cows and miscarried cows at 18, 21, 33, 39, and 51 d after artificial insemination. And 15, 101, 1, 2, and 2 DEGs were upregulated, and 17, 74, 4, and 8 DEGs were downregulated in the cows in the pregnant and miscarriage groups, respectively at 18, 21, 33, and 39, but no gene was downregulated at 51 d after artificial insemination. These DEGs were distributed to 13, 20, 3, 6, and 20 pathways, and some pathway essential for pregnancy, such as cell adhesion molecules, tumor necrosis factor signaling pathway and PI3K-Akt signaling pathway. Conclusion: This analysis has identified several genes and related pathways crucial for pregnancy and miscarriage in cows, as well as these genes supply molecular markers to predict the miscarriage in cows.

Keywords

References

  1. Bai R, Kusama K, Sakurai T, et al. The role of endometrial selectins and their ligands on bovine conceptus attachment to the uterine epithelium during peri-implantation period. Biol Reprod 2015;93:46. https://doi.org/10.1095/biolreprod.115.128652
  2. Dunne LD, Diskin MG, Sreenan JM. Embryo and foetal loss in beef heifers between day 14 of gestation and full term. Anim Reprod Sci 2000;58:39-44. https://doi.org/10.1016/S0378-4320(99)00088-3
  3. Berg DK, van Leeuwen J, Beaumont S, Berg M, Pfeffer PL. Embryo loss in cattle between Days 7 and 16 of pregnancy. Theriogenology 2010;73:250-60. https://doi.org/10.1016/j.theriogenology.2009.09.005
  4. Diskin MG, Parr MH, Morris DG. Embryo death in cattle: an update. Reprod Fertil Dev 2011;24:244-51. https://doi.org/10.1071/RD11914
  5. Carracedo S, Sacher F, Brandes G, Braun U, Leitges M. Redundant role of protein kinase C delta and epsilon during mouse embryonic development. PLoS One 2014;9:e103686. https://doi.org/10.1371/journal.pone.0103686
  6. Mitko K, Ulbrich SE, Wenigerkind H, et al. Dynamic changes in messenger RNA profiles of bovine endometrium during the oestrous cycle. Reproduction 2008;135:225-40. https://doi.org/10.1530/REP-07-0415
  7. Bai R, Bai H, Kuse M, et al. Involvement of VCAM1 in the bovine conceptus adhesion to the uterine endometrium. Reproduction 2014;148:119-27. https://doi.org/10.1530/REP-13-0655
  8. Li Q, Davila J, Bagchi MK, Bagchi IC. Chronic exposure to bisphenol a impairs progesterone receptor-mediated signaling in the uterus during early pregnancy. Receptors Clin Investig 2016;3:e1369.
  9. Sasaki H. Roles and regulations of Hippo signaling during preimplantation mouse development. Dev Growth Differ 2017;59:12-20. https://doi.org/10.1111/dgd.12335
  10. Newcomer BW, Cofield LG, Walz PH, Givens MD. Prevention of abortion in cattle following vaccination against bovine herpesvirus 1: A meta-analysis. Prev Vet Med 2017;138:1-8. https://doi.org/10.1016/j.prevetmed.2017.01.005
  11. Terefe Y, Girma S, Mekonnen N, Asrade B. Brucellosis and associated risk factors in dairy cattle of eastern Ethiopia. Trop Anim Health Prod 2017;49:599-606. https://doi.org/10.1007/s11250-017-1242-7
  12. Garrett JE, Geisert RD, Zavy MT, Morgan GL. Evidence for maternal regulation of early conceptus growth and development in beef cattle. J Reprod Fertil 1988;84:437-46. https://doi.org/10.1530/jrf.0.0840437
  13. Carter F, Forde N, Duffy P, et al. Effect of increasing progesterone concentration from day 3 of pregnancy on subsequent embryo survival and development in beef heifers. Reprod Fertil Dev 2008;20:368-75. https://doi.org/10.1071/RD07204
  14. Clemente M, de La Fuente J, Fair T, et al. Progesterone and conceptus elongation in cattle: a direct effect on the embryo or an indirect effect via the endometrium? Reproduction 2009; 138:507-17. https://doi.org/10.1530/REP-09-0152
  15. Bauersachs S, Ulbrich SE, Gross K, et al. Embryo-induced transcriptome changes in bovine endometrium reveal species-specific and common molecular markers of uterine receptivity. Reproduction 2006;132:319-31. https://doi.org/10.1530/rep.1.00996
  16. Rhinehart JD, Starbuck-Clemmer MJ, Flores JA, et al. Low peripheral progesterone and late embryonic/early fetal loss in suckled beef and lactatingdairy cows. Theriogenology 2009; 71:480-90. https://doi.org/10.1016/j.theriogenology.2008.07.031
  17. Rao X, Huang X, Zhou Z, Lin X. An improvement of the $2^{(-ΔΔCT)}$ method for quantitative real-time polymerase chain reaction data analysis. Biostat Bioinforma Biomath 2013;3: 71-85.
  18. Wolf E, Arnold GJ, Bauersachs S, et al. Embryo-maternal communication in bovine - strategies for deciphering a complex cross-talk. Reprod Domest Anim 2003;38:276-89. https://doi.org/10.1046/j.1439-0531.2003.00435.x
  19. Forde N, Carter F, Fair T, et al. Progesterone-regulated changes in endometrial gene expression contribute to advanced conceptus development in cattle. Biol Reprod 2009;81:784-94. https://doi.org/10.1095/biolreprod.108.074336
  20. Carter F, Rings F, Mamo S, et al. Effect of elevated circulating progesterone concentration on bovine blastocyst development and global transcriptome following endoscopic transfer of in vitro produced embryos to the bovine oviduct. Biol Reprod 2010;83:707-19. https://doi.org/10.1095/biolreprod.109.082354
  21. Clemente M, Lopez-Vidriero I, O’Gaora P, et al. Transcriptome changes at the initiation of elongation in the bovine conceptus. Biol Reprod 2011;85:285-95. https://doi.org/10.1095/biolreprod.111.091587
  22. Imakawa K, Bai R, Fujiwara H, et al. Continuous model of conceptus implantation to the maternal endometrium. J Endocrinol 2017;233:R53-R65. https://doi.org/10.1530/JOE-16-0490
  23. Psychoyos A. Hormonal control of ovoimplantation. Vitam Horm 1973;31:201-56. https://doi.org/10.1016/S0083-6729(08)60999-1
  24. Yamakoshi S, Bai R, Chaen T, et al. Expression of mesenchymal-related genes by the bovine trophectoderm following conceptus attachment to the endometrial epithelium. Reproduction 2012;143:377-87. https://doi.org/10.1530/REP-11-0364
  25. Diao H, Aplin JD, Xiao S, et al. Altered spatiotemporal expression of collagen types I, III, IV, and VI in Lpar3-deficient peri-implantation mouse uterus. Biol Reprod 2011;84:255-65. https://doi.org/10.1095/biolreprod.110.086942
  26. Sponchiado M, Gomes NS, Fontes PK, et al. Pre-hatching embryo-dependent and -independent programming of endometrial function in cattle. PLoS One 2017;12:e0175954. https://doi.org/10.1371/journal.pone.0175954
  27. Bai R, Bai H, Kuse M, et al. Involvement of VCAM1 in the bovine conceptus adhesion to the uterine endometrium. Reproduction 2014;148:119-27. https://doi.org/10.1530/REP-13-0655
  28. Sasaki H. Roles and regulations of Hippo signaling during preimplantation mouse development. Dev Growth Differ 2017; 59:12-20. https://doi.org/10.1111/dgd.12335