Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Cell Surface Antigen Display for Neuronal Differentiation-Specific Tracking

  • Kim, Sang Chul (Graduate School of Biomedical Science and Engineering, Hanyang University) ;
  • Lee, Eun-Hye (Hanyang Biomedical Research Institute, Hanyang University) ;
  • Yu, Ji Hea (Department and Research Institute of Rehabilitation Medicine, Yonsei University College of Medicine) ;
  • Kim, Sang-Mi (Hanyang Biomedical Research Institute, Hanyang University) ;
  • Nam, Bae-Geun (Department and Research Institute of Rehabilitation Medicine, Yonsei University College of Medicine) ;
  • Chung, Hee Yong (Graduate School of Biomedical Science and Engineering, Hanyang University) ;
  • Kim, Yeon-Soo (Department of New Drug Discovery & Development, Graduate School of New Drug Discovery & Development, Chungnam National University) ;
  • Cho, Sung-Rae (Department and Research Institute of Rehabilitation Medicine, Yonsei University College of Medicine) ;
  • Park, Chang-Hwan (Graduate School of Biomedical Science and Engineering, Hanyang University)
  • Received : 2018.06.19
  • Accepted : 2018.09.27
  • Published : 2019.01.01
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Abstract

Cell therapeutic agents for treating degenerative brain diseases using neural stem cells are actively being developed. However, few systems have been developed to monitor in real time whether the transplanted neural stem cells are actually differentiated into neurons. Therefore, it is necessary to develop a technology capable of specifically monitoring neuronal differentiation in vivo. In this study, we established a system that expresses cell membrane-targeting red fluorescent protein under control of the Synapsin promoter in order to specifically monitor differentiation from neural stem cells into neurons. In order to overcome the weak expression level of the tissue-specific promoter system, the partial 5' UTR sequence of Creb was added for efficient expression of the cell surface-specific antigen. This system was able to track functional neuronal differentiation of neural stem cells transplanted in vivo, which will help improve stem cell therapies.

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References

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