한방비만학회지 (Journal of Korean Medicine for Obesity Research)

  • 제19권2호
  • /
  • Pages.106-112
  • /
  • 2019
  • /
  • 1976-9334(pISSN)
  • /
  • 2288-1522(eISSN)
한방비만학회 (The Society of Korean Medicine for obesity Research)
권호 표지
DOI QR코드

DOI QR Code

성숙한 3T3-L1 지방세포에서 Akkermansia muciniphila의 지방축적 감소 효과

Effect of Reduction in the Adipose Accumulation of Akkermansia muciniphila in Mature 3T3-L1 Adipocytes

  • 심혜윤 (동국대학교 한의과대학 한방재활의학교실) ;
  • 임수경 (동국대학교 한의과대학 한방재활의학교실) ;
  • 신주현 ((주)엔테로바이옴) ;
  • 이도경 ((주)엔테로바이옴) ;
  • 서재구 ((주)엔테로바이옴) ;
  • 김호준 (동국대학교 한의과대학 한방재활의학교실)
  • Shim, Hyeyoon (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Dongguk University) ;
  • Lim, Sookyoung (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Dongguk University) ;
  • Shin, Joo-Hyun (R&D Center, Enterobiome Inc.) ;
  • Lee, Dokyung (R&D Center, Enterobiome Inc.) ;
  • Seo, Jae-Gu (R&D Center, Enterobiome Inc.) ;
  • Kim, Hojun (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Dongguk University)
  • 투고 : 2019.10.31
  • 심사 : 2019.12.05
  • 발행 : 2019.12.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objectives: The aim of this study was to observe the reduction of lipid accumulation by treatment with Akkermansia muciniphila extract on 3T3-L1 adipocytes. Methods: After treating pasteurized Akk. muciniphila strains in HT-29 colorectal cancer cell, the relative expression of interleukin (IL)-8, tumor necrosis factor-α, IL-6, and IL-1β mRNA was analyzed by real time polymerase chain reaction, respectively. 27 strains of Akk. muciniphila which have anti-inflammatory effects were selected. 3T3-L1 pre-adipocytes were treated with Akk. muciniphila for 24 hr and then measured the toxicity using water soluble tetrazolium salt assay. The cells were incubated for 4 days and then differentiated into adipocytes using the medium including adipogenic reagents for 10 days. The Akk. muciniphila was treated when the medium was exchanged for differentiation medium at 4th day and insulin medium at 6th day. To observe the lipid accumulation, the cells were stained with Oil red O dye and were measured using a spectrophotometer. Results: In the cytotoxicity test, the cell viability of 3T3-L1 pre-adipocytes was significantly increased compared to the control group which untreated with Akk. muciniphila, and there was no cytotoxicity of Akk. muciniphila at 1×107 CFU/mL. The results on Oil red O staining and absorbance measurements were showed a significant decrease in lipid accumulation in the group which was treated with Akk. muciniphila compared to the control group. Conclusions: In our results, Akk. muciniphila has the inhibitory effect of lipid accumulation in 3T3-L1 adipocytes. This suggests that Akk. muciniphila could be help to improve obesity.

키워드

참고문헌

  1. Vallianou N, Stratigou T, Christodoulatos GS, Dalamaga M. Understanding the role of the gut microbiome and microbial metabolites in obesity and obesity-associated metabolic disorders: current evidence and perspectives. Current Obesity Reports. 2019 ; 8 : 1-16. https://doi.org/10.1007/s13679-019-0325-3
  2. John GK, Mullin GE. The gut microbiome and obesity. Current Oncology Reports. 2016 ; 18(7) : 45. https://doi.org/10.1007/s11912-016-0528-7
  3. Tilg H, Kaser A. Gut microbiome, obesity, and metabolic dysfunction. The Journal of Clinical Investigation. 2011 ; 121(6) : 2126-32. https://doi.org/10.1172/JCI58109
  4. Dahiya DK, Renuka, Puniya M, Shandilya UK, Dhewa T, Kumar N, et al. Gut microbiota modulation and its relationship with obesity using prebiotic fibers and probiotics: a review. Front Microbiol. 2017 ; 8 : 563. https://doi.org/10.3389/fmicb.2017.00563
  5. Sivamaruthi BS, Kesika P, Suganthy N, Chaiyasut C. A review on role of microbiome in obesity and antiobesity properties of probiotic supplements. BioMed Research International. 2019 ; 2019 : 3291367.
  6. Dao MC, Everard A, Aron-Wisnewsky J, Sokolovska N, Prifti E, Verger EO, et al. Akkermansia muciniphila and improved metabolic health during a dietary intervention in obesity: relationship with gut microbiome richness and ecology. Gut. 2016 ; 65(3) : 426-36. https://doi.org/10.1136/gutjnl-2014-308778
  7. Derrien M, Vaughan EE, Plugge CM, de Vos WM. Akkermansia muciniphila gen. nov., sp. nov., a human intestinal mucin-degrading bacterium. International Journal of Systematic and Evolutionary Microbiology. 2004 ; 54(5) : 1469-76. https://doi.org/10.1099/ijs.0.02873-0
  8. Derrien M, Van Baarlen P, Hooiveld G, Norin E, Muller M, de Vos W. Modulation of mucosal immune response, tolerance, and proliferation in mice colonized by the mucin-degrader Akkermansia muciniphila. Frontiers in Microbiology. 2011 ; 2 : 166. https://doi.org/10.3389/fmicb.2011.00166
  9. Zhao S, Liu W, Wang J, Shi J, Sun Y, Wang W, et al. Akkermansia muciniphila improves metabolic profiles by reducing inflammation in chow diet-fed mice. Journal of Molecular Endocrinology. 2017 ; 58(1) : 1-14. https://doi.org/10.1530/JME-16-0054
  10. Cani PD, de Vos WM. Next-generation beneficial microbes: the case of Akkermansia muciniphila. Frontiers in Microbiology. 2017 ; 8 : 1765. https://doi.org/10.3389/fmicb.2017.01765
  11. Plovier H, Everard A, Druart C, Depommier C, Van Hul M, Geurts L, et al. A purified membrane protein from Akkermansia muciniphila or the pasteurized bacterium improves metabolism in obese and diabetic mice. Nat Med. 2017 ; 23(1) : 107-13. https://doi.org/10.1038/nm.4236
  12. Kim HW, B aeg MK, Kim JH. The r ole of the gut m icrobiota in obesity. The Korean Journal of Medicine. 2019 ; 94(5) : 410-3. https://doi.org/10.3904/kjm.2019.94.5.410
  13. Derrien M, Belzer C, de Vos WM. Akkermansia muciniphila and its role in regulating host functions. Microb Pathog. 2017 ; 106 : 171-81. https://doi.org/10.1016/j.micpath.2016.02.005
  14. Ottman N, Geerlings SY, Aalvink S, de Vos WM, Belzer C. Action and function of Akkermansia muciniphila in microbiome ecology, health and disease. Best Practice & Research Clinical Gastroenterology. 2017 ; 31(6) : 637-42. https://doi.org/10.1016/j.bpg.2017.10.001
  15. Rajilic-Stojanovic M, Shanahan F, Guarner F, de Vos WM. Phylogenetic analysis of dysbiosis in ulcerative colitis during remission. Inflamm Bowel Dis. 2013 ; 19(3) : 481-8. https://doi.org/10.1097/MIB.0b013e31827fec6d
  16. Depommier C, Everard A, Druart C, Plovier H, Van Hul M, Vieira-Silva S, et al. Supplementation with Akkermansia muciniphila in overweight and obese human volunteers: A proof-of-concept exploratory study. Nature Medicine. 2019 ; 25(7) : 1096. https://doi.org/10.1038/s41591-019-0495-2

피인용 문헌

  1. Beneficial Effects of Newly Isolated Akkermansia muciniphila Strains from the Human Gut on Obesity and Metabolic Dysregulation vol.8, pp.9, 2019, https://doi.org/10.3390/microorganisms8091413
  2. 최근 10년간 한방비만학회지의 연구동향 분석: 2010-2019년 한방비만학회지 게재논문을 중심으로 vol.20, pp.2, 2019, https://doi.org/10.15429/jkomor.2020.20.2.149