한방비만학회지 (Journal of Korean Medicine for Obesity Research)

  • 제19권2호
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  • Pages.89-96
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  • 2019
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  • 1976-9334(pISSN)
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  • 2288-1522(eISSN)
한방비만학회 (The Society of Korean Medicine for obesity Research)
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무막줄기세포추출물의 3T3-L1 세포에서 포도당 흡수 촉진 효과

Membrane Free Stem Cell Extract from Adipose Tissue Enhances Glucose Uptake in 3T3-L1 Cells

  • Kim, Ji Hyun (Department of Food Science and Nutrition, Pusan National University) ;
  • Kim, Min Jeong (Department of Food Science and Nutrition, Pusan National University) ;
  • Park, Hye Sook (T-STEM Co., Ltd.) ;
  • Kim, Young Sil (T-STEM Co., Ltd.) ;
  • Cho, Eun Ju (Department of Food Science and Nutrition, Pusan National University)
  • 투고 : 2019.10.01
  • 심사 : 2019.10.28
  • 발행 : 2019.12.30
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초록

Objectives: We investigated whether membrane free stem cell extract from adipose tissue (MFSCE) has anti-diabetic effect. Methods: To determine glucose uptake effect of MFSCE, we carried out glucose uptake assay in 3T3-L1 adipocytes. The regulatory mechanisms of MFSCE on glucose uptake were examined by Western blot analysis. Results: When MFSCE was treated to adipocytes at the concentration of 0.5, 1, 2.5, and 5 ㎍/mL, 2-deoxyglucose-6-phosphate uptake was elevated approximately 1.8-fold compared to cells not treated with MFSCE. It indicated that MFSCE enhances glucose uptake in 3T3-L1 adipocytes. In addition, MFSCE reduced phosphorylation of insulin receptor substrate-1 at serine 307 and induced Akt and glucose transporter 4 protein expressions that were related to insulin signaling. Furthermore, MFSCE regulated adenosine monophosphate-activated protein kinase (AMPK) pathway by increases of increase phosphorylation of AMPK and acetyl-CoA carboxylase that were related to AMPK pathway. Conclusions: These results indicated that MFSCE promotes glucose uptake via modulation of insulin signaling and AMPK pathway. Therefore, MFSCE could be a promising agent for treatment of diabetes mellitus.

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참고문헌

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피인용 문헌

  1. 최근 10년간 한방비만학회지의 연구동향 분석: 2010-2019년 한방비만학회지 게재논문을 중심으로 vol.20, pp.2, 2019, https://doi.org/10.15429/jkomor.2020.20.2.149