Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
권호 표지
DOI QR코드

DOI QR Code

Sequence analysis of the fusion protein gene of Newcastle disease virus isolated from breeder ducks in Korea

  • Han, Mi Na (Chungcheongbuk-do Veterinary Service Laboratory) ;
  • Byeon, Hyeon Seop (Chungcheongbuk-do Veterinary Service Laboratory) ;
  • Lee, Cho Yeon (Chungcheongbuk-do Veterinary Service Laboratory) ;
  • Jo, Nam Sin (Chungcheongbuk-do Veterinary Service Laboratory) ;
  • Lee, Jong Hwa (Chungcheongbuk-do Veterinary Service Laboratory) ;
  • Jang, Rae Hoon (Chungcheongbuk-do Veterinary Service Laboratory) ;
  • Kim, Chang Seop (Chungcheongbuk-do Veterinary Service Laboratory) ;
  • Na, Ki Jeong (College of Veterinary Medicine, Chungbuk National University)
  • Received : 2019.11.28
  • Accepted : 2019.12.24
  • Published : 2019.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

Newcastle disease (ND) is an infectious poultry disease that caused high mortality and reduced egg production. NDVs are regularly present in the domestic duck population. And ducks play a possible role in the maintenance and transmission of NDVs. While we were monitoring the Avian Influenza, NDVs were isolated from field samples by accident. So we analysed the biological and genetic characteristics of these viruses. Lentogenic NDVs were isolated from two farms among twenty breeder duck farms. The ages of ducks were 39 weeks old in the 'A' farm and 3~72 weeks old in the 'B' farm. And they were not inoculated with the NDVs vaccine. In the biological characteristics, the both viruses which separated from the farm 'A' and 'B' were thermostable. The amino acid sequence of a site from 112 to 119 in the fusion (F) protein was 'GKQGRLIG' which has monobasic motif in the samples of both farms. And this means the separated NDVs are lentogenic. Phylogenetic analysis was performed by entire nucleotide sequence of F protein. The virus strains from the A farm (MN095239) and the B farm (MN095240) belonged to class II genotype I. Using the analysis of whole F protein nucleic acid sequence, the MN095239 (GenBank) had homology with Ulster strain about 99.95% and the MN095239 (GenBank) had homology with KR/CK/KU_LBM255/09 strain about 99.89%. NDV surveillance is needed to investigate epidemiological relationship of domestic breeder duck isolates in Korea.

Keywords

References

  1. Aldous EW, Myun JK Banks J, Alexander DJ. 2003. A molecular epidemiological study of avian paramyxovirus type 1 (Newcastle disease virus) isolates by phylogenetic analysis of a partial nucleotide sequence of the fusion protein gene. Avian Pathology 32: 239-256. https://doi.org/10.1080/030794503100009783
  2. Alexander DJ. 1988b. Newcastle disease diagnosis. In: Alexander DJ. (Ed.), Newcastle Disease. Kluwer Academic Publishers, Boston, 147-60.
  3. Anonymous. 2006. Newcastle disease. In OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals (chapter 2.1.15). Paris: World Organisation for Animal Health
  4. Bello MB, Yusoff KM, Ideris A, Hair-Bejo M, Peeters BPH, Jibril AH, Tambuwal FM, Omar AR. 2018. Genotype Diversity of Newcastle Disease Virus in Nigeria: Disease Control Challenges and Future Outlook. Adv Virol. 2018 Dec 2
  5. Brauer R, Chen P. Influenza virus propagation in embryonated chicken eggs. 2015. J Vis Exp. 2015 Mar, 19;(97).
  6. Dai Y, Cheng X, Liu M, Shen X, Li J, Yu S, Zou J, Ding C. 2014. Experimental infection of duck origin virulent Newcastle disease virus strain in ducks. BMC Vet Res. 2014 Jul 17;10: 164. https://doi.org/10.1186/1746-6148-10-164
  7. de Almeida RS, Hammoumi S, Gil P, Briand FX, Molia S, Gaidet N, Cappelle J, Chevalier V, Balanca G, Traore A, Grillet C, Maminiaina OF, Guendouz S, Dakouo M, Samake K, Bezeid Oel.M, Diarra A, Chaka H, Goutard F, Thompson P, Martinez D, Jestin V, Albina E. 2013. New avian paramyxoviruses type I strains identified in Africa provide new outcomes for phylogeny reconstruction and genotype classification. PLoS One. 2013;8(10):e76413. https://doi.org/10.1371/journal.pone.0076413
  8. Dimitrov KM, Abolnik C, Afonso CL, Albina E, Bahl J, Berg M, Briand FX, Brown IH, Choi KS, Chvala I, Diel DG. 2019. Updated unified phylogenetic classification system and revised nomenclature for Newcastle disease virus. Infection, Genetics and Evolution, 74: 103917. https://doi.org/10.1016/j.meegid.2019.103917
  9. Dimitrov KM, Ramey AM, Qiu X, Bahl J, Afonso CL. 2016. Temporal, geographic, and host distribution of avian paramyxovirus 1 (Newcastle disease virus). Infect Genet Evol 39: 22-34 https://doi.org/10.1016/j.meegid.2016.01.008
  10. Guan Y, Zheng BJ, He YQ, Liu XL, Zhuang ZX, Cheung CL, Luo SW, Li PH, Zhang LJ, Guan YJ, Butt KM, Wong KL, Chan KW, Lim W, Shortridge KF, Yuen Peiris JS, Poon LL. 2005. Isolation and characterization of viruses related to the SARS coronavirus from animals in southern China. Science 302: 276-278. https://doi.org/10.1126/science.1087139
  11. ICTV. 2018. Order Mononegavirales; family Paramyxoviridae; genus Avulavirus. Virus taxonomy: 2018a release. EC 50, Washington, DC, July 2018; email ratification February 2019 (MSL #34).
  12. Kabilov MR, Alikina TY, Yurchenko KS, Glushchenko AV, Gunbin KV, Shestopalov AM, Gubanova NV. 2016. Complete genome sequences of two Newcastledisease virus strains isolated from a wild duck and a pigeon in Russia. Genome Announc. 4: e01348-16.
  13. Kattenbelt JA, Stevens MP, Gould AR. 2006. Sequence variation in the Newcastle disease virus genome. Virus Res. 116: 168-184. https://doi.org/10.1016/j.virusres.2005.10.001
  14. Kim BY, Lee DH, Kim MS, Jang JH, Lee YN, Park JK, Yuk SS, Lee JB, Park SY, Choi IS, Song CS. 2012. Exchange of Newcastle disease viruses in Korea: The relatedness of isolates between wild birds, live bird markets, poultry farms and neighboring countries. Infect. Genet. Evol. 12: 478-482. https://doi.org/10.1016/j.meegid.2011.12.004
  15. Kim LM, King DJ, Curry PE, Suarez DL, Swayne DE, Stallknecht DE, Slemons RD, Pedersen JC, Senne DA, Winker K, Afonso CL. 2007. Phylogenetic diversity among low virulence Newcastle disease viruses from waterfowl and shorebirds and comparison of genotype distributions to poultry-origin isolates. J Virol 81: 12641-12653. https://doi.org/10.1128/JVI.00843-07
  16. Kim LM, King DJ, Suarez DL, Wong CW, Afonso CL. 2007. Characterization of class I Newcastle disease virus isolates from Hong Kong live bird markets and detection using real-time reverse transcription-PCR. Journal of Clinical Microbiology 45: 1310-1314 https://doi.org/10.1128/JCM.02594-06
  17. Kim SH, Wanasen N, Paldurai A, Xiao S, Collins PL, Samal SK. 2013. Newcastle disease virus fusion protein is the major contributor to protective immunity of genotype-matched vaccine.
  18. Lee EK, Jeon WJ, Kim JW, Park MJ, Moon SH, Lee SH, Kwon JH, Choi KS. 2009a. Characterization of lentogenic Newcastle disease virus isolated in Jeju, Korea during 2006-2007 surveillance. J Bacteriol Virol 39: 383-393 https://doi.org/10.4167/jbv.2009.39.4.383
  19. Lee EK, Jeon WJ, Kwon JH, Yang CB, Choi KS. 2009b. Molecular epidemiological investigation of Newcastle disease virus from domestic duck in Korea. Vet Microbiol 134: 241-248 https://doi.org/10.1016/j.vetmic.2008.08.020
  20. Lee HJ, Kwon JS, Lee DH, Lee YN, Youn HN, Lee YJ, Kim MC, Jeong OM, Kang HM, Kwon JH, Lee JB, Park SY, Choi IS, Song CS. 2010. Continuing evolution and interspecies transmission of influenza viruses in live bird markets in Korea. Avian Dis. 54: 738-748. https://doi.org/10.1637/8785-040109-ResNote.1
  21. Lee YJ, Sung HW, Choi JG, Kim JH, Song CS. 2004. Molecular epidemiology of Newcastle disease viruses isolated in South Korea using sequencing of the fusion protein cleavage site region and phylogenetic relationships. Avian Pathol. 33: 482-91. 10 https://doi.org/10.1080/03079450400003700
  22. Liu X, Wang X, Wu S, Hu S, Peng Y, Xue F, Liu X. 2009. Surveillance for avirulent Newcastle disease viruses in domestic ducks (Anas platyrhynchos and Cairina moschata) at live bird markets in Eastern China and characterization of the viruses isolated. Avian Pathol. 38: 377-391. https://doi.org/10.1080/03079450903183637
  23. Patti J. Miller. 2016. Newcastle Disease in Poultry. MSD Veterinary Manual. 11th edition.
  24. Seal BS, Wise MG, Pedersen JC, Senne DA, Alvarez R, Scott MS, King DJ, Yu Q, Kapczynski DR. 2005. Genomic sequences of low-virulence avian paramyxovirus-1 (Newcastle disease virus) isolates obtained from livebird markets in North America not related to commonly utilized commercial vaccine strains.
  25. Seal BS. 2004. Nucleotide and predicted amino acid sequence analysis of the fusion protein and hemagglutinin-neuraminidase protein genes among Newcastle disease virus isolates. Phylogenetic relationships among the Paramyxovirinae based on attachment glycoprotein sequences. Functional & Integrative Genomics 4: 246-257 https://doi.org/10.1007/s10142-004-0113-2
  26. Snoeck CJ, Adeyanju AT, Owoade Aa. et al. 2013. Genetic diversity of newcastle disease virus in wild birds and pigeons in West Africa. Applied and Environmental Microbiology. 2013;79(24): 7867-7874. https://doi.org/10.1128/AEM.02716-13
  27. Wang J, Lv Y, Zhang Y, Zheng D, Zhao Y, Castellan D, Liu H, Wang Z. 2016. Genomic characterizations of a newcastle disease virus isolated from ducks in live bird markets in China. PLoS One 11: e0158771 https://doi.org/10.1371/journal.pone.0158771
  28. Webster RG. 2004. Wet markets - A continuing source of severe acute respiratory syndrome and influenza? Lancet 363: 234-236. https://doi.org/10.1016/S0140-6736(03)15329-9
  29. Westbury H. 2001. Newcastle disease virus: an evolving pathogen? Avian Pathol. 30: 5-11 https://doi.org/10.1080/03079450020023131