Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Cytotoxicity of target cell against Naegleria fowleri under non-contact condition

비접촉 조건에서의 Naegleria fowleri에 의한 표적세포의 세포독성

  • Kang, Changgeun (Institute of Animal Medicine, Gyeongsang National University) ;
  • Hong, Il-Hwa (Institute of Animal Medicine, Gyeongsang National University) ;
  • Kim, Jong-Hyun (Institute of Animal Medicine, Gyeongsang National University)
  • 강창근 (경상대학교 동물의학연구소) ;
  • 홍일화 (경상대학교 동물의학연구소) ;
  • 김종현 (경상대학교 동물의학연구소)
  • Received : 2019.02.07
  • Accepted : 2019.09.30
  • Published : 2019.12.30
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Abstract

Naegleria fowleri, a pathogenic free-living amoeba, leads to a fatal infection known as primary amebic meningoencephalitis (PAM) in human and animals. PAM is an acute, fulminant, necrotizing, and hemorrhagic disease that leads to death in approximately seven days. In this study, we investigate the cytotoxicity of target cells and the secreted molecules of N. fowleri under the non-contact condition. The target cell (U87MG cell) treated with N. fowleri lysates showed no morphological changes and no cytotoxicity. By contrast, the U87MG cells co-cultured with N. fowleri trophozoites under the non-contact condition induced morphological changes and reduction in number. When U87MG cells were co-cultured with N. fowleri trophozoites under the non-contact condition for 30 min, 2 hr, and 4 hr, the levels of cytotoxicity of target cells were 32.3, 35.5, and 37.8%, respectively. Particularly, when the ratio of amoeba to target cells is 10 to 1, the level of cytotoxicity of target cells was 49.7% at 30 min. To show the proteins secreted from N. fowleri under the non-contact condition, we carried out 2D electrophoresis and observed 6 major proteins. Finally, these results suggest that the molecules released from N. fowleri under the non-contact condition induce the cell death and this process is an important step in pathogenesis of N. fowleri.

Keywords

References

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