Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Asiatic Acid Induces Apoptosis and Autophagy and Reduces MiR-17 and MiR-21 Expression in Pancreatic Cancer Cell Lines

  • Jo, Yoon-Gyung (College of Pharmacy and Wonkwang Oriental Medicines Research Institute, Wonkwang University) ;
  • Kim, Myoungjae (College of Pharmacy and Wonkwang Oriental Medicines Research Institute, Wonkwang University) ;
  • Shin, Hyeji (College of Pharmacy, Korea University) ;
  • Lee, Ki Yong (College of Pharmacy, Korea University) ;
  • Lee, Eun Joo (College of Pharmacy and Wonkwang Oriental Medicines Research Institute, Wonkwang University)
  • Received : 2019.10.21
  • Accepted : 2019.11.29
  • Published : 2019.12.31
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Abstract

This study investigated the cytotoxic effects and mechanism of action of asiatic acid in pancreatic cancer cell lines. First, we confirmed the cell viability of MIA PaCa-2 and PANC-1 cells after asiatic acid administration for 48 and 72 h. The viability of MIA PaCa-2 and PANC-1 cells decreased in a dose-dependent manner following asiatic acid administration. To investigate the underlying mechanism, we performed a terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, annexin V assay, and western blotting. Asiatic acid induced apoptosis and autophagy through activation of AMP-activated protein kinase (AMPK) and inhibition of mammalian target of rapamycin (mTOR) in MIA PaCa-2 cells. Finally, the expression of miR-17 and miR-21, known as oncogenes in pancreatic cancer, was decreased by asiatic acid. These results indicate that asiatic acid has potential as a new therapeutic agent against pancreatic cancer.

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References

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