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진딧물 전반 딸기 바이러스 발생조사 및 딸기모틀바이러스의 계통분석

Incidence of Aphid-Transmitted Strawberry Viruses in Korea and Phylogenetic Analysis of Korean Isolates of Strawberry Mottle Virus

  • 권선정 (농촌진흥청 국립원예특작과학원 원예특작환경과) ;
  • 윤정범 (농촌진흥청 국립원예특작과학원 원예특작환경과) ;
  • 조인숙 (농촌진흥청 국립원예특작과학원 원예특작환경과) ;
  • 윤주연 (농촌진흥청 국립원예특작과학원 원예특작환경과) ;
  • 권태룡 (농촌진흥청 국립원예특작과학원 원예특작환경과)
  • Kwon, Sun-Jung (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Yoon, Jung-Beom (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Cho, In-Sook (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Yoon, Ju-Yeon (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kwon, Tae-Ryong (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, RDA)
  • 투고 : 2019.11.02
  • 심사 : 2019.11.27
  • 발행 : 2019.12.31

초록

딸기를 감염하는 30여 종의 바이러스 중 전 세계적으로 가장 많이 발생하는 4종의 바이러스는 Strawberry mild yellow edge virus (SMYEV), Strawberry mottle virus (SMoV), Strawberry crinkle virus (SCV), Strawberry vein banding virus (SVBV)로 이들은 모두 진딧물이 전반되며 경제적으로 가장 중요한 바이러스들이다. 2018-2019년까지 국내 주요 딸기 생산지에서 국내 딸기 품종을 대상으로 이들 4종 진딧물 전반 바이러스의 발생조사를 실시하였다. 그 결과, 일부 국내 딸기 품종에서 SMYEV와 SMoV가 각각 0.7%와 1.3%의 낮은 감염률로 검출되었으며 SCV와 SVBV는 전혀 검출되지 않았다. 한편, 바이러스 감염 식물에서 병징은 관찰되지 않았다. 국내에서 SMoV에 대한 염기서열은 보고된 바 없어 SMoV 국내분리주에 대한 3' untranslated region의 염기서열을 결정하고 분석하였다. 기존에 보고된 SMoV 분리주들과의 분자계통학적 유연관계 분석 결과, 대부분의 국내 분리주는 캐나다 분리주와 근연관계가 높은 것으로 나타났으며 염기서열의 진화적 측면에서 분화가 거의 일어나지 않은 분리주임을 확인하였다.

Among more than 30 viruses infecting strawberry, aphid-transmitted viruses including Strawberry mild yellow edge virus (SMYEV), Strawberry mottle virus (SMoV), Strawberry crinkle virus (SCV), and Strawberry vein banding virus (SVBV) have been considered as the most economically important viruses of strawberry in the world. To determine the incidence of these four viruses in major Korean strawberry cultivars, field surveys were conducted in major production areas during 2018-2019. In our surveys, SMYEV and SMoV were detected with low infection rates of 0.7% and 1.3%, respectively, whereas SCV and SVBV were not detected. No obvious symptoms were observed in the strawberry plants infected by SMYEV or SMoV. Since no sequences of SMoV Korean isolates have been reported, we initially determined nucleotide sequence of the 3' untranslated region (UTR) of seven SMoV isolates obtained during the surveys. The 3' UTR sequences (782 nt) of seven Korean isolates were phylogenetically compared with those of the previously reported SMoV isolates. Phylogenetic analysis revealed that most Korean isolates are closely related to Canadian isolates and only little evolutionary differentiation occurred among the Koreans isolates. This might be due to the low incidence of SMoV in strawberry in Korea.

키워드

참고문헌

  1. Bhagwat, B., Dickison, V., Su, L., Bernardy, M., Wiersma, P. A., Nie, X. et al. 2016. Molecular characterization of divergent strawberry mild yellow edge virus isolates from Eastern Canada. J. Phytopathol. 164: 691-696. https://doi.org/10.1111/jph.12444
  2. Cho, J.-D., Choi, G.-S., Chung, B.-N., Kim, J.-S. and Choi, H.-S. 2011. Strawberry mild yellow edge potexvirus from strawberry in Korea. Plant Pathol. J. 27: 187-190. https://doi.org/10.5423/PPJ.2011.27.2.187
  3. Choi, G.-S., Lee, J.-A., Cho, J.-D., Chung, B.-N., Cho, I.-S. and Kim, J.-S. 2009. Strawberry virus diseases occurring in Korea, 2007-2008. Res. Plant Dis. 15: 8-12. (In Korean) https://doi.org/10.5423/RPD.2009.15.1.008
  4. Franova, J. 2001. Occurrence of graft-transmissible virus diseases of the strawberry in the Czech Republic. Acta Virol. 45: 151-157.
  5. Frazier, N. W. 1974. Six new strawberry indicator clones evaluated for the detection and diagnosis of twelve graft-transmissible diseases. Plant Dis. Rep. 58: 28-31.
  6. Freeman, J. A. and Mellor, F. C. 1962. Influence of latent viruses on vigor, yield and quality of British Sovereign strawberries. Can. J. Plant Sci. 42: 602-610. https://doi.org/10.4141/cjps62-103
  7. Horn, N. L. and Carver, R. G. 1962. Effect of three viruses on plant production and yields of strawberries. Plant Dis. Rep. 46: 762-765.
  8. Krczal, H. 1982. Investigation on the biology of the strawberry aphid (Chaetosiphon fragaefolii), the most important vector of strawberry viruses in West Germany. Acta Hortic. 129: 63-68. https://doi.org/10.17660/actahortic.1982.129.11
  9. Kwon, S.-J., Cho, I.-S., Yoon, J.-Y. and Choi, G.-S. 2018. Genetic diversity of two isolates in strawberry mild yellow edge virus from Korea. Res. Plant Dis. 24: 285-291. (In Korean) https://doi.org/10.5423/RPD.2018.24.4.285
  10. Lee, W., Kim, H., Lim, J., Choi, H.-R., Kim, Y., Kim, Y.-S. et al. 2011. Barcoding aphids (Hemiptera: Aphididae) of the Korean Peninsula: updating the global data set. Mol. Ecol. Resour. 11: 32-37. https://doi.org/10.1111/j.1755-0998.2010.02877.x
  11. Martin, R. R. and Tzanetakis, I. E. 2006. Characterization and recent advances in detection of strawberry viruses. Plant Dis. 90: 384-396. https://doi.org/10.1094/PD-90-0384
  12. Mellor, F. C. and Krczal, H. 1987. Strawberry mottle. In: Virus Disease of Small Fruits, Agricultural Handbook, No. 631, ed. by R. H. Converse, pp. 10-16. United States Department of Agriculture, Agricultural Research Service, Washington, DC, USA.
  13. Spiegel, S. and Martin, R. R. 1998. Virus and viruslike diseases. In: Compendium of Strawberry Disease, ed. by J. L. Maas, pp. 62-72. 2nd ed. American Phytopathological Society, St. Paul, MN, USA.
  14. Staudt, G. 1962. Taxonomic studies in the genus Fragaria. Typification of Fragaria species known at the time of Linnaeus. Can. J. Bot. 40: 869-886. https://doi.org/10.1139/b62-081
  15. Thompson, J. R. and Jelkmann, W. 2003. The detection and variation of Strawberry mottle virus. Plant Dis. 87: 385-390. https://doi.org/10.1094/PDIS.2003.87.4.385
  16. Thompson, J. R., Leone, G., Lindner, J. L., Jelkmann, W. and Schoen, C. D. 2002. Characterization and complete nucleotide sequence of Strawberry mottle virus: a tentative member of a new family of bipartite plant picorna-like viruses. J. Gen. Virol. 83: 229-239. https://doi.org/10.1099/0022-1317-83-1-229
  17. Yang, H., Zhang, Z., Dai, H. and Li, H. 2007. Detection of strawberry mottle virus by RT-PCR. Acta Hortic. 764: 127-136. https://doi.org/10.17660/actahortic.2007.764.15