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Screening of 56 Herbal formulas covered by the National Health Insurance Service on Dementia-related Factors

국민 건강보험 급여 한약 처방 56종의 치매 주요 생리지표 및 신경세포 변화에 대한 효능 비교 연구

  • Lim, Hye-Sun (Clinical Medicine Division, Korea Institute of Oriental Medicine) ;
  • Kim, Yu Jin (Clinical Medicine Division, Korea Institute of Oriental Medicine) ;
  • Kim, Yoon ju (Clinical Medicine Division, Korea Institute of Oriental Medicine) ;
  • Kim, Bu-Yeo (Clinical Medicine Division, Korea Institute of Oriental Medicine) ;
  • Jeong, Soo-Jin (Clinical Medicine Division, Korea Institute of Oriental Medicine)
  • 임혜선 (한국한의학연구원 임상의학부) ;
  • 김유진 (한국한의학연구원 임상의학부) ;
  • 김윤주 (한국한의학연구원 임상의학부) ;
  • 김부여 (한국한의학연구원 임상의학부) ;
  • 정수진 (한국한의학연구원 임상의학부)
  • Received : 2018.07.12
  • Accepted : 2018.09.07
  • Published : 2018.09.30

Abstract

Objectives: The purpose of this study was to investigate the effects of 56 herbal formulae covered by the National Health Insurance Corporation (NHIC) on dementia-related biomarkers and neuronal cell changes. Methods: The 56 herbal formulae were extracted with 70% ethanol at $100^{\circ}C$ for 2 h. The antioxidant properties was measured by radical scavenging assay using ABTS+ radical. The acetylcholinesterase (AChE) activity was tested by Ellman's assay and $amyloid-{\beta}$ ($A{\beta}$) aggregation was determined using fluorescence method. To estimate the inhibitory effects of herbal formulae on neuronal cell death and inflammation using HT22 hippocampal cells and BV-2 microglia, respectively. Results: Among the 56 herbal formulae, Dangguiyukhwangtang, Banhasasimtang, Samhwangsasimtang, Cheongwiesan, Hwangryunhaedoktang, Banhabaekchulchunmatang, Jaeumganghwatang, Cheongseoikgitang, and Hoechunyanggyuksan has a significant inhibitory effects on acetylcholinesterase (AChE) activity. Doinseunggitang and Samhwangsasimtang exerted the effect on the inhibition of $amyloid-{\beta}$ ($A{\beta}$) aggregation. Additionally, 10 herbal formulae affected AChE and $A{\beta}$ aggregation revealed antioxidant activity as well as neuroprotective and anti-neuroinflammation effects in neuronal cell lines. Conclusions: 10 herbal formulae that have been shown to be effective against the major dementia markers have been shown to have antioxidant activity, neuronal cell protection and inhibition of brain inflammation. Further investigation of these herbal formulae will need to be validated in dementia animal models.

Keywords

References

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