Clinical and Experimental Reproductive Medicine

  • 제45권3호
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  • Pages.129-134
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  • 2018
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  • 2233-8233(pISSN)
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  • 2233-8241(eISSN)
대한생식의학회 (The Korean Society for Reproductive Medicine)
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Thinning and drilling laser-assisted hatching in thawed embryo transfer: A randomized controlled trial

  • Le, Minh Tam (Center for Reproductive Endocrinology and Infertility (HUECREI), Hue University of Medicine and Pharmacy) ;
  • Nguyen, Thi Tam An (Center for Reproductive Endocrinology and Infertility (HUECREI), Hue University of Medicine and Pharmacy) ;
  • Nguyen, Thi Thai Thanh (Center for Reproductive Endocrinology and Infertility (HUECREI), Hue University of Medicine and Pharmacy) ;
  • Nguyen, Van Trung (Center for Reproductive Endocrinology and Infertility (HUECREI), Hue University of Medicine and Pharmacy) ;
  • Le, Dinh Duong (Department of Public Health, Hue University of Medicine and Pharmacy) ;
  • Nguyen, Vu Quoc Huy (Department of Obstetrics and Gynecology, Hue University of Medicine and Pharmacy) ;
  • Cao, Ngoc Thanh (Center for Reproductive Endocrinology and Infertility (HUECREI), Hue University of Medicine and Pharmacy) ;
  • Aints, Alar (Institute of Clinical Medicine, Department of Obstetrics and Gynecology, University of Tartu) ;
  • Salumets, Andres (Institute of Clinical Medicine, Department of Obstetrics and Gynecology, University of Tartu)
  • 투고 : 2018.05.14
  • 심사 : 2018.07.04
  • 발행 : 2018.09.30
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초록

Objective: In frozen and thawed embryos, the zona pellucida (ZP) can be damaged due to hardening. Laser-assisted hatching (LAH) of embryos can increase the pregnancy rate. This study compared thinning and drilling of the ZP before frozen embryo transfer (FET). Methods: Patients were randomly allocated into two groups for LAH using thinning or drilling on day 2 after thawing. Twenty-five percent of the ZP circumference and 50% of the ZP thickness was removed in the thinning group, and a hole $40{\mu}m$ in diameter was made in the drilling group. Results: A total of 171 in vitro fertilization/intracytoplasmic sperm injection FET cycles, including 85 cycles with drilling LAH and 86 cycles with thinning LAH, were carried out. The thinning group had a similar ${\beta}$-human chorionic gonadotropin-positive rate (38.4% vs. 29.4%), implantation rate (16.5% vs. 14.4%), clinical pregnancy rate (36.0% vs. 25.9%), miscarriage rate (5.8% vs. 2.4%), ongoing pregnancy rate (30.2% vs. 23.5%), and multiple pregnancy rate (7.0% vs. 10.6%) to the drilling LAH group. There were no significant differences in pregnancy outcomes between subgroups defined based on age (older or younger than 35 years) or ZP thickness (greater or less than $17{\mu}m$) according to the LAH method. Conclusion: The present study demonstrated that partial ZP thinning or drilling resulted in similar outcomes in implantation and pregnancy rates using thawed embryos, irrespective of women's age or ZP thickness.

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참고문헌

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피인용 문헌

  1. Application of femtosecond laser microsurgery in assisted reproductive technologies for preimplantation embryo tagging vol.10, pp.6, 2019, https://doi.org/10.1364/boe.10.002985
  2. Laser-assisted hatching in lower grade cleavage stage embryos improves blastocyst formation: results from a retrospective study vol.14, pp.1, 2018, https://doi.org/10.1186/s13048-021-00844-7