Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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Effects of platelet-rich plasma on tooth replantation in dogs: a histologic and histomorphometric analysis

  • Yang, Jun-Mo (Department of Periodontology, Chosun University School of Dentistry) ;
  • Yang, Keon-Il (Department of Periodontology, Chosun University School of Dentistry) ;
  • Lee, Kyung-Hyun (Department of Periodontology, Chosun University School of Dentistry) ;
  • Choi, Seong-Ho (Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University School of Dentistry) ;
  • Kim, Byung-Ock (Department of Periodontology, Chosun University School of Dentistry) ;
  • Park, Joo-Cheol (Department of Oral Histology/Developmental Biology, Dental Research Institute, Seoul National University School of Dentistry) ;
  • Yu, Sang-Joun (Department of Periodontology, Chosun University School of Dentistry)
  • Received : 2018.07.15
  • Accepted : 2018.08.19
  • Published : 2018.08.30
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Abstract

Purpose: The purpose of this study was to evaluate the effects of platelet-rich plasma (PRP) on periodontal healing of replanted root surfaces in dogs histologically and histomorphometrically. Methods: A total of 36 roots of mandibular incisors and premolars from 6 mongrel dogs were used. The roots were randomly divided into 3 groups: 1) a positive control group (n=12), in which the periodontal ligament (PDL) and cementum were retained and the roots were soaked in saline; 2) a negative control group (n=12), in which the PDL and cementum were removed and the roots were soaked in saline; and 3) an experimental group (n=12), in which the PDL and cementum were removed and the roots were soaked in PRP. After soaking the root surfaces, the extracted roots were replanted into the extraction sockets. The roots were covered using a coronally repositioned flap Results: Histologically, irregular-thickness PDL-like and cementum-like tissues were observed in the 4-week experimental group and the positive control group. PDL-like tissue and cementum-like tissue with a more uniform thickness were observed at 8 weeks. In the negative control group, PDL-like tissue and cementum-like tissue were rarely found, and root resorption and ankylosis were observed. In the cross-sectional histomorphometric analysis, the experimental group demonstrated a higher rate of formation of cementum-like tissue and a lower tooth ankylosis rate than the positive and negative control groups at 4 and 8 weeks. Although there was a significant difference in the tooth ankylosis rate and the formation of cementum-like tissue across the 3 groups (P<0.05), no statistical significance was observed between any pair of groups (P>0.017). Conclusions: Applying PRP to root surfaces during tooth replantation in dogs can reduce tooth ankylosis and increase PDL-like and cementum-like tissue formation.

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References

  1. Grossman LI. Intentional replantation of teeth. J Am Dent Assoc 1966;72:1111-8. https://doi.org/10.14219/jada.archive.1966.0125
  2. Madison S. Intentional replantation. Oral Surg Oral Med Oral Pathol 1986;62:707-9. https://doi.org/10.1016/0030-4220(86)90268-9
  3. Weine FS. The case against intentional replantation. J Am Dent Assoc 1980;100:664-8. https://doi.org/10.14219/jada.archive.1980.0227
  4. Lu DP. Intentional replantation of periodontally involved and endodontically mistreated tooth. Oral Surg Oral Med Oral Pathol 1986;61:508-13. https://doi.org/10.1016/0030-4220(86)90396-8
  5. Demiralp B, Nohutcu RM, Tepe DI, Eratalay K. Intentional replantation for periodontally involved hopeless teeth. Dent Traumatol 2003;19:45-51. https://doi.org/10.1034/j.1600-9657.2003.00084.x
  6. Frechette JP, Martineau I, Gagnon G. Platelet-rich plasmas: growth factor content and roles in wound healing. J Dent Res 2005;84:434-9. https://doi.org/10.1177/154405910508400507
  7. Cho MI, Lin WL, Genco RJ. Platelet-derived growth factor-modulated guided tissue regenerative therapy. J Periodontol 1995;66:522-30. https://doi.org/10.1902/jop.1995.66.6.522
  8. Park JB, Matsuura M, Han KY, Norderyd O, Lin WL, Genco RJ, et al. Periodontal regeneration in class III furcation defects of beagle dogs using guided tissue regenerative therapy with platelet-derived growth factor. J Periodontol 1995;66:462-77. https://doi.org/10.1902/jop.1995.66.6.462
  9. Jin Q, Anusaksathien O, Webb SA, Printz MA, Giannobile WV. Engineering of tooth-supporting structures by delivery of PDGF gene therapy vectors. Mol Ther 2004;9:519-26. https://doi.org/10.1016/j.ymthe.2004.01.016
  10. Saygin NE, Tokiyasu Y, Giannobile WV, Somerman MJ. Growth factors regulate expression of mineral associated genes in cementoblasts. J Periodontol 2000;71:1591-600. https://doi.org/10.1902/jop.2000.71.10.1591
  11. Takayama S, Murakami S, Miki Y, Ikezawa K, Tasaka S, Terashima A, et al. Effects of basic fibroblast growth factor on human periodontal ligament cells. J Periodontal Res 1997;32:667-75. https://doi.org/10.1111/j.1600-0765.1997.tb00577.x
  12. Terranova VP, Odziemiec C, Tweden KS, Spadone DP. Repopulation of dentin surfaces by periodontal ligament cells and endothelial cells. Effect of basic fibroblast growth factor. J Periodontol 1989;60:293-301. https://doi.org/10.1902/jop.1989.60.6.293
  13. Fujii S, Maeda H, Tomokiyo A, Monnouchi S, Hori K, Wada N, et al. Effects of TGF-${\beta}1$ on the proliferation and differentiation of human periodontal ligament cells and a human periodontal ligament stem/progenitor cell line. Cell Tissue Res 2010;342:233-42. https://doi.org/10.1007/s00441-010-1037-x
  14. Moore YR, Dickinson DP, Wikesjo UM. Growth/differentiation factor-5: a candidate therapeutic agent for periodontal regeneration? A review of pre-clinical data. J Clin Periodontol 2010;37:288-98. https://doi.org/10.1111/j.1600-051X.2009.01527.x
  15. Caceres M, Hidalgo R, Sanz A, Martinez J, Riera P, Smith PC. Effect of platelet-rich plasma on cell adhesion, cell migration, and myofibroblastic differentiation in human gingival fibroblasts. J Periodontol 2008;79:714-20. https://doi.org/10.1902/jop.2008.070395
  16. Marx RE, Carlson ER, Eichstaedt RM, Schimmele SR, Strauss JE, Georgeff KR. Platelet-rich plasma: growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1998;85:638-46. https://doi.org/10.1016/S1079-2104(98)90029-4
  17. Tozum TF, Demiralp B. Platelet-rich plasma: a promising innovation in dentistry. J Can Dent Assoc 2003;69:664.
  18. Dohan DM, Choukroun J, Diss A, Dohan SL, Dohan AJ, Mouhyi J, et al. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part II: platelet-related biologic features. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;101:e45-50. https://doi.org/10.1016/j.tripleo.2005.07.009
  19. Shanaman R, Filstein MR, Danesh-Meyer MJ. Localized ridge augmentation using GBR and platelet-rich plasma: case reports. Int J Periodontics Restorative Dent 2001;21:345-55.
  20. Wiltfang J, Schlegel KA, Schultze-Mosgau S, Nkenke E, Zimmermann R, Kessler P. Sinus floor augmentation with $\beta$-tricalciumphosphate ($\beta$-TCP): does platelet-rich plasma promote its osseous integration and degradation? Clin Oral Implants Res 2003;14:213-8. https://doi.org/10.1034/j.1600-0501.2003.140212.x
  21. Camargo PM, Lekovic V, Weinlaender M, Vasilic N, Madzarevic M, Kenney EB. Platelet-rich plasma and bovine porous bone mineral combined with guided tissue regeneration in the treatment of intrabony defects in humans. J Periodontal Res 2002;37:300-6. https://doi.org/10.1034/j.1600-0765.2002.01001.x
  22. Srinivas BV, Rupa N, Halini Kumari KV, Prasad SS, Varalakshmi U, Sudhakar K. Root coverage using subepithelial connective tissue graft with platelet-rich plasma in the treatment of gingival recession: a clinical study. J Pharm Bioallied Sci 2015;7:S530-8. https://doi.org/10.4103/0975-7406.163530
  23. Petrungaro PS. Treatment of the infected implant site using platelet-rich plasma. Compend Contin Educ Dent 2002;23:363-6, 368.
  24. Polimeni G, Xiropaidis AV, Wikesjo UM. Biology and principles of periodontal wound healing/regeneration. Periodontol 2000 2006;41:30-47. https://doi.org/10.1111/j.1600-0757.2006.00157.x
  25. Chen FM, Chen R, Wang XJ, Sun HH, Wu ZF. In vitro cellular responses to scaffolds containing two microencapulated growth factors. Biomaterials 2009;30:5215-24. https://doi.org/10.1016/j.biomaterials.2009.06.009
  26. Park SY, Kim KH, Shin SY, Koo KT, Lee YM, Seol YJ. Dual delivery of rhPDGF-BB and bone marrow mesenchymal stromal cells expressing the BMP2 gene enhance bone formation in a critical-sized defect model. Tissue Eng Part A 2013;19:2495-505. https://doi.org/10.1089/ten.tea.2012.0648
  27. Chan CP, Lin CP, Chang MC, Hsieh CC, Hsu CC, Lin CL, et al. Effects of thrombin on the growth, protein synthesis, attachment, clustering and alkaline phosphatase activity of cultured human periodontal ligament fibroblasts. Proc Natl Sci Counc Repub China B 1998;22:137-43.
  28. Reichert da Silva Assuncao L, Colenci R, Ferreira do-Amaral CC, Sonoda CK, Mogami Bomfim SR, Okamoto R, et al. Periodontal tissue engineering after tooth replantation. J Periodontol 2011;82:758-66. https://doi.org/10.1902/jop.2010.100448
  29. Zhao YH, Zhang M, Liu NX, Lv X, Zhang J, Chen FM, et al. The combined use of cell sheet fragments of periodontal ligament stem cells and platelet-rich fibrin granules for avulsed tooth reimplantation. Biomaterials 2013;34:5506-20. https://doi.org/10.1016/j.biomaterials.2013.03.079
  30. Andersson L, Jonsson BG, Hammarstrom L, Blomlof L, Andreasen JO, Lindskog S. Evaluation of statistics and desirable experimental design of a histomorphometrical method for studies of root resorption. Endod Dent Traumatol 1987;3:288-95. https://doi.org/10.1111/j.1600-9657.1987.tb00637.x
  31. Andreasen JO. Effect of extra-alveolar period and storage media upon periodontal and pulpal healing after replantation of mature permanent incisors in monkeys. Int J Oral Surg 1981;10:43-53. https://doi.org/10.1016/S0300-9785(81)80007-5
  32. Andreasen JO, Borum MK, Jacobsen HL, Andreasen FM. Replantation of 400 avulsed permanent incisors. 4. Factors related to periodontal ligament healing. Endod Dent Traumatol 1995;11:76-89. https://doi.org/10.1111/j.1600-9657.1995.tb00464.x

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