Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
권호 표지
DOI QR코드

DOI QR Code

Apoptotic effect of physcion isolated from marine fungus Microsporum sp. in PC3 human prostate cancer cells

  • Ding, Yi-Shan (Department of Marine-Bio Convergence Science, Pukyong National University) ;
  • Kim, Won-Suk (Major in Pharmaceutical Engineering Division of Bio-industry, Silla University) ;
  • Park, Sun Joo (Department of Chemistry, Pukyong National University) ;
  • Kim, Se-Kwon (Department of Marine Life Science, College of Ocean Science and Technology, Korea Maritime and Ocean University)
  • Received : 2018.02.01
  • Accepted : 2018.05.16
  • Published : 2018.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

Background: Apoptosis is a process of programmed cell death, and apoptosis defect results in serious diseases such as cancer. Apoptosis induction is one of the key mechanisms of anti-cancer agents. This study was aimed to find anti-prostate cancer compounds from marine-derived fungus Microsporum sp. Results: We found that physcion isolated from the fermentation broth extract of the marine fungus Microsporum sp. strain MFS-YL decreases the cell proliferation of PC3 human prostate cancer cells. Physcion induced cell apoptosis as determined by Annexin V/propidium iodide double staining. Physcion downregulated the anti-apopotoic proteins such as Ras, Bcl-xL, and Bcl-2, whereas upregulated the pro-apoptotic Bax. Physcion also activated caspase-3, caspase-8, and caspase-9. Conclusion: These results suggest that physcion from Microsporum sp. inhibits the proliferation of PC3 human prostate cancer cells via the pathway leading to apoptotic cell death. Physcion may be a potential candidate in the field of anticancer drug discovery against human prostate cancer.

Keywords

References

  1. Almeida AP, Dethoup T, Singburaudom N, Lima R, Vasconcelos MH, Pinto M, Kijjoa A. The in vitro anticancer activity of the crude extract of the spongeassociated fungus Eurotium cristatum and its secondary metabolites. J Nat Pharma. 2010;1:25-9. https://doi.org/10.4103/2229-5119.73583
  2. Attaay DH, Zaborsky OR. Marine biotechnology pharmaceutical and bioactive natural products. New York: Plenum Press; 1993. p. 125-9.
  3. Blunt JW, Copp BR, Keyzers RA, Munro MH, Prinsep MR. Marine natural products. Nat Prod Rep. 2014;31:160-258. https://doi.org/10.1039/c3np70117d
  4. Chen X, Gao H, Han Y, Ye J, Xie J, Wang C. Physcion induces mitochondria-driven apoptosis in colorectal cancer cells via downregulating EMMPRIN. Eur J Pharmacol. 2015;764:124-33. https://doi.org/10.1016/j.ejphar.2015.07.008
  5. Elf S, Lin S, Xia Y, Pan C, Shan S, Wu S, Lonial M, Gaddh ML, Arellano HJ, Khoury FR, Khuri BH, Lee TJ, Boggon J, Chen J. Targeting 6-phosphogluconate dehydrogenase in the oxidative PPP sensitizes leukemia cells to antimalarial agent dihydroartemisinin. Oncogene. 2017;36:254-62. https://doi.org/10.1038/onc.2016.196
  6. Feldman BJ, Feldman D. The development of androgen-independent prostate cancer. Nat Rev Cancer. 2001;1:34-45. https://doi.org/10.1038/35094009
  7. Grossmann ME, Huang H, Tindall DJ. Androgen receptor signaling in androgenrefractory prostate cancer. J Natl Cancer Inst. 2001;93:1687-97. https://doi.org/10.1093/jnci/93.22.1687
  8. Guo S, Feng B, Zhu R, Ma J, Wang W. Preparative isolation of three anthraquinones from Rumex japonicus by high-speed counter-current chromatography. Mol. 2011;16:1201-10. https://doi.org/10.3390/molecules16021201
  9. Gurumuthy S, Vasudevan KM, Ranqnekar VM. Regulation of apoptosis in prostate cancer. Cancer Metastasis Rev. 2001;20:225-43. https://doi.org/10.1023/A:1015583310759
  10. Hetz CA, Torres V, Quest AF. Beyond apoptosis: nonapoptotic cell death in physiology and disease. Biochem Cell Biol. 2005;83:579-88. https://doi.org/10.1139/o05-065
  11. Hong JY, Chung HJ, Bae SY, Trung TN, Bae K, Lee SK. Induction of cell cycle arrest and apoptosis by physcion, an anthraquinone isolated from rhubarb (rhizomes of Rheum tanguticum), in MDA-MB-231 human breast cancer cells. J Cancer Prev. 2014;19:273-8. https://doi.org/10.15430/JCP.2014.19.4.273
  12. Karran L, Dyer MJ. Proteolytic cleavage of molecules involved in cell death or survival pathways: a role in the control of apoptosis? Crit Rev Eukaryot Gene Expr. 2001;11:269-77.
  13. Kyprianou N, Tu H, Jacobs SC. Apoptotic versus proliferative activities in human benign prostatic hyperplasia. Hum Pathol. 1996;27:668-75. https://doi.org/10.1016/S0046-8177(96)90396-2
  14. Li JL, Wang AQ, Li JS, He WY, Kong M. Studies on the anthraquinones of Hotao Rhubarb (Pheum hotaoense). Chin Tradit Herb Drugs. 2000;31:321-3.
  15. Li Y, Li XF, Son BW, Choi HD. Screening of antimicrobial activity from the marinederived fungus. Korean J Pharmacology. 2003;34:142-4.
  16. Linke SP, Clarkin KC, Wahl GM. p53 mediates permanent arrest over multiple cell cycles in response to gamma-irradiation. Cancer Res. 1997;57:1171-9.
  17. Liu W, He J, Yang Q, Guo Q, Guo F. Upregulating miR-146a by physcion reverses multidrug resistance in human chronic myelogenous leukemia K562/AMD cells. Am J Cancer Res. 2016;6:2547-60.
  18. Lowe SW, Lin AW. Apoptosis in cancer. Carcinogenesis. 2000;21:485-95. https://doi.org/10.1093/carcin/21.3.485
  19. Madan RA, Pal SK, Sartor O, Dahut WL. Overcoming chemotherapy resistance in prostate cancer. Clin Cancer Res. 2011;17:3892-902. https://doi.org/10.1158/1078-0432.CCR-10-2654
  20. Mayer AM, Gustafson KR. Marine pharmacology in 2000: antitumor and cytotoxic compounds. Int J Cancer. 2003;105:291-9. https://doi.org/10.1002/ijc.11080
  21. Naito K, Fukuda Y, Toyoda Y. Effects of porcine follicular fluid on male pronucleus formation in porcine oocytes matured in vitro. Gamete Res. 1988;21:289-95. https://doi.org/10.1002/mrd.1120210310
  22. Smetanina OF, Kalinovsky AI, Khudyakova YV, Pivkin MV, Dmitrenok PS, Fedorov SN, Ji H, Kwak JY, Kuznetsova TA. Indole alkaloids produced by a marine fungus isolate of Penicillium janthinellum Biourge. J Nat Prod. 2007;70:906-9. https://doi.org/10.1021/np060396d
  23. Spierings D, McStay G, Saleh M, Bender C, Chipuk J, Maurer U, Green DR. Connected to death: the (unexpurgated) mitochondrial pathway of apoptosis. Science. 2005;310:66-7. https://doi.org/10.1126/science.1117105
  24. Stegh AH, Peter ME. Apoptosis and caspases. Cardiol Clin. 2001;19:13-29. https://doi.org/10.1016/S0733-8651(05)70192-2
  25. Wijesekara I, Kim SK. Angiotensin-I-converting enzyme (ACE) inhibitors from marine resources: prospects in the pharmaceutical industry. Mar Drugs. 2010;31:1080-93.
  26. Wijesekara I, Zhang C, Van Ta Q, Vo TS, Li YX, Kim SK. Physcion from marine-derived fungus Microsporum sp. induces apoptosis in human cervical carcinoma HeLa cells. Microbiol Res. 2014;169:255-61. https://doi.org/10.1016/j.micres.2013.09.001
  27. Yang F, Zhang T, Tian G, Cao H, Liu Q, Ito Y. Preparative isolation and purification of hydroxyanthraquinones from Rheum officinale Baill by high-speed countercurrent chromatography using pH-modulated stepwise elution. J Chromatogr A. 1999;858:103-7. https://doi.org/10.1016/S0021-9673(99)00827-4

Cited by

  1. Petromurin C Induces Protective Autophagy and Apoptosis in FLT3-ITD-Positive AML: Synergy with Gilteritinib vol.18, pp.1, 2018, https://doi.org/10.3390/md18010057
  2. Potential of Anti-Cancer Activity of Secondary Metabolic Products from Marine Fungi vol.7, pp.6, 2018, https://doi.org/10.3390/jof7060436
  3. Physcion ameliorates pancreatic β-cell dysfunction and diabetes-related oxidative stress markers in type 2 diabetes rat model vol.1, pp.4, 2018, https://doi.org/10.1016/j.phyplu.2021.100114