Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Association of leptin, toll-like receptor 4, and chemokine receptor of interleukin 8 C-X-C motif single nucleotide polymorphisms with fertility traits in Czech Fleckvieh cattle

  • Received : 2017.12.12
  • Accepted : 2018.04.02
  • Published : 2018.11.01
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Abstract

Objective: The use of genetic markers can help to enhance reproduction in cattle, which is a very important trait for profitability in dairy production systems. This study evaluated the association between genotypes of leptin (LEP), toll-like receptor 4 (TLR4), and chemokine receptor of interleukin 8 C-X-C motif (CXCR1) genes and fertility traits in Czech Fleckvieh cattle. Methods: Phenotypic data from 786 Czech Fleckvieh cows raised on 5 farms in the Czech Republic were used, along with information from the 1st three parities. To determine genotype, the polymerase chain reaction-restriction fragment length polymorphism method was used. Results: Except for LEP g.-963C>T, all studied genotype frequencies of single nucleotide polymorphisms (SNPs) were distributed according to the Hardy-Weinberg equilibrium. Two LEP SNPs (g.-963C>T and c.357C>T) were associated with the age at the 1st calving, days open (DO), pregnancy rate after 1st service (PR), and calving interval (CLI). In LEP g.-963C>T the TT genotype heifers firstly calved 24 days earlier than CC genotype and the CT genotype cow showed a tendency for shorter DO and higher PR. In LEP c.357C>T we observed longer CLI and DO period in TT cows. In general, we can propose the TT genotype of g.-963C>T as favorable and the TT genotype of c.357C>T as unfavorable for a cow's fertility. Heterozygotes in TLR4 c.-226C>G were significantly associated with shorter CLI, and presented a nonsignificant tendency to be associated with higher PR. In CXCR1 c.777 C>G, we did not observe any relationship of this SNP with reproduction. Conclusion: Overall, the results showed that LEP could be an effective marker for improving reproduction in Czech Fleckvieh cattle. This study also provides novel insights into the relationship between TLR4 and CXCR1 SNPs and reproduction in dual-purpose cattle.

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References

  1. Chessa S, Nicolazzi EL, Nicoloso L, et al. Analysis of candidate SNPs affecting milk and functional traits in the dual-purpose Italian Simmental cattle. Livest Sci 2015;173:1-8. https://doi.org/10.1016/j.livsci.2014.12.015
  2. Liefers SC, Veerkamp RF, Pas M, et al. Leptin promoter mutations affect leptin levels and performance traits in dairy cows. Anim Genet 2005;36:111-8. https://doi.org/10.1111/j.1365-2052.2005.01246.x
  3. Clempson AM, Pollott GE, Brickell JS, et al. Evidence that leptin genotype is associated with fertility, growth, and milk production in Holstein cows. J Dairy Sci 2011;94:3618-28. https://doi.org/10.3168/jds.2010-3626
  4. Komisarek J. Impact of LEP and LEPR gene polymorphisms on functional traits in Polish Holstein-Friesian cattle. Anim Sci Pap Rep 2010;28:133-41.
  5. Haegeman A, Zeveren AV, Peelman LJ. New mutation in exon 2 of the bovine leptin gene. Anim Genet 2000;31:79. https://doi.org/10.1111/j.1365-2052.2000.579-14.x
  6. Glantz M, Mansson HL, Stalhammar H, Paulsson M. Effect of polymorphisms in the leptin, leptin receptor and acyl-CoA: diacylglycerol acyltransferase 1 (DGAT1) genes and genetic polymorphism of milk proteins on bovine milk composition. J Dairy Res 2012;79:110-8. https://doi.org/10.1017/S0022029911000859
  7. Sharma BS, Mount J, Karrow NA. Functional characterization of a single nucleotide polymorphism in the 5' UTR region of the bovine toll-like receptor 4 gene. Dev Biol 2008;132:331-6.
  8. Akira S, Takeda K. Toll-like receptor signalling. Nat Rev Immunol 2004;4:499-511. https://doi.org/10.1038/nri1391
  9. Fu Y, Liu B, Feng X, et al. Lipopolysaccharide increases Tolllike receptor 4 and downstream Toll-like receptor signaling molecules expression in bovine endometrial epithelial cells. Vet Immunol Immunopathol 2013;151:20-7. https://doi.org/10.1016/j.vetimm.2012.09.039
  10. Herath S, Lilly ST, Santos NR, et al. Expression of genes associated with immunity in the endometrium of cattle with disparate postpartum uterine disease and fertility. Reprod Biol Endocrinol 2009;7:55. https://doi.org/10.1186/1477-7827-7-55
  11. Sominsky L, Sobinoff AP, Jobling MS, et al. Immune regulation of ovarian development: programming by neonatal immune challenge. Front Neurosci 2013;7:100.
  12. Prakash O, Kumar A, Sonwane A, et al. Polymorphism of cytokine and innate immunity genes associated with bovine brucellosis in cattle. Mol Biol Rep 2014;41:2815-25. https://doi.org/10.1007/s11033-014-3136-3
  13. Oviedo-Boyso J, Valdez-Alarcon JJ, Cajero-Juarez M. Innate immune response of bovine mammary gland to pathogenic bacteria responsible for mastitis. J Infect 2007;54:399-409. https://doi.org/10.1016/j.jinf.2006.06.010
  14. Youngerman SM, Saxton AM, Oliver SP, Pighetti GM. Association of CXCR2 polymorphisms with subclinical and clinical mastitis in dairy cattle. J Dairy Sci 2004;87:2442-8. https://doi.org/10.3168/jds.S0022-0302(04)73367-6
  15. Beecher C, Daly M, Childs S, et al. Polymorphisms in bovine immune genes and their associations with somatic cell count and milk production in dairy cattle. BMC Genet 2010;11:99.
  16. Galvao KN, Pighetti GM, Cheong SH, Nydam DV, Gilbert RO. Association between interleukin-8 receptor-alpha (CXCR1) polymorphism and disease incidence, production, reproduction, and survival in Holstein cows. J Dairy Sci 2011;94:2083-91. https://doi.org/10.3168/jds.2010-3636
  17. Verbeke J, Van Poucke M, Peelman L, Piepers S, De Vliegher S. Associations between CXCR1 polymorphisms and pathogenspecific incidence rate of clinical mastitis, test-day somatic cell count, and test-day milk yield. J Dairy Sci 2014;97:7927-39. https://doi.org/10.3168/jds.2014-8216
  18. Komisarek J, Antkowiak I. The relationship between leptin gene polymorphisms and reproductive traits in Jersey cows. Pol J Vet Sci 2007;10:193-7.
  19. Carvajal AM, Huircan P, Lepori A. Single nucleotide polymorphisms in immunity-related genes and their association with mastitis in Chilean dairy cattle. Genet Mol Res 2013;12:2702-11. https://doi.org/10.4238/2013.July.30.8
  20. Goertz I, Baes C, Weimann C, Reinsch N, Erhardt G. Association between single nucleotide polymorphisms in the CXCR1 gene and somatic cell score in Holstein dairy cattle. J Dairy Sci 2009;92:4018-22. https://doi.org/10.3168/jds.2008-1536
  21. Peakall R, Smouse PE. Genalex 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes 2006;6:288-95. https://doi.org/10.1111/j.1471-8286.2005.01155.x
  22. Peakall R, Smouse PE. GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research-an update. Bioinformatics 2012;28:2537-9. https://doi.org/10.1093/bioinformatics/bts460
  23. Choi Y, Sims GE, Murphy S, Miller JR, Chan AP. Predicting the functional effect of amino acid substitutions and indels. PloS One 2012;7:e46688. https://doi.org/10.1371/journal.pone.0046688
  24. Thomas PD, Campbell MJ, Kejariwal A, et al. PANTHER: a library of protein families and subfamilies indexed by function. Genome Res 2003;13:2129-41. https://doi.org/10.1101/gr.772403
  25. Capriotti E, Fariselli P, Casadio R. I-Mutant2.0: predicting stability changes upon mutation from the protein sequence or structure. Nucleic Acids Res 2005;33;W306-10. https://doi.org/10.1093/nar/gki375
  26. Jecminkova K, Kyselova J, Said AA, et al. Leptin promoter region genotype frequencies and its variability in the Czech Fleckvieh cattle. Sci Agric Bohem 2016;47:54-9.
  27. Yazdani H, Rahmani HR, Edris MA, Dirandeh E. Association between A59V polymorphism in exon 3 of leptin gene and reproduction traits in cows of Iranian Holstein. Afr J Biotechnol 2010;9:5997-6000.
  28. Szyda J, Morek-Kopec M, Komisarek J, Zarnecki A. Evaluating markers in selected genes for association with functional longevity of dairy cattle. BMC Genet 2011;12:30.
  29. Griesbeck-Zilch B, Meyer HHD, Kuhn C, Schwerin M, Wellnitz O. Staphylococcus aureus and Escherichia coli cause deviating expression profiles of cytokines and lactoferrin messenger ribonucleic acid in mammary epithelial cells. J Dairy Sci 2008;91:2215-24. https://doi.org/10.3168/jds.2007-0752
  30. Petzl W, Zerbe H, Gunther J, et al. Escherichia coli, but not Staphylococcus aureus triggers an early increased expression of factors contributing to the innate immune defense in the udder of the cow. Vet Res 2008;39:18. https://doi.org/10.1051/vetres:2007057
  31. Fontanesi L, Cal DG, Galimberti G, et al. A candidate gene association study for nine economically important traits in Italian Holstein cattle. Anim Genet 2014;45:576-80. https://doi.org/10.1111/age.12164
  32. Pryce JE, Royal MD, Garnsworthy PC, Mao IL. Fertility in the high-producing dairy cow. Livest Prod Sci 2004;86:125-35. https://doi.org/10.1016/S0301-6226(03)00145-3
  33. Bagheri M, Moradi-Sharhrbabak M, Miraie-Ashtiani R, Safdari-Shahroudi M, Abdollahi-Arpanahi R. Case-control approach application for finding a relationship between candidate genes and clinical mastitis in holstein dairy cattle. J Appl Genet 2016;57:107-12. https://doi.org/10.1007/s13353-015-0299-0