Oral Biology Research (구강생물연구)

The Institute of Dental Science, Chosun University (조선대학교 치의학연구원)
권호 표지
DOI QR코드

DOI QR Code

Surface characteristics and bioactivity of minocycline-treated Ti-6Al-4V alloy

  • Lee, Jung-Hyuk (Department of Periodontology, School of Dentistry, Chonnam National University) ;
  • Sun, Young-Gon (Department of Periodontology, School of Dentistry, Chonnam National University) ;
  • Na, Eui-Ri (Department of Periodontology, School of Dentistry, Chonnam National University) ;
  • Moon, Jong-Wook (Department of Periodontology, School of Dentistry, Chonnam National University) ;
  • Kim, Young-Joon (Department of Periodontology, School of Dentistry, Chonnam National University)
  • Received : 2018.06.21
  • Accepted : 2018.10.04
  • Published : 2018.12.31
⟨ Previous Next ⟩

Abstract

Chemical agents such as minocycline (MC) and citric acid (CA) were suggested in the treatment of contaminated implant surface. In this study, MC-HCl treatment was performed to enhance surface characteristics of titanium alloy surface. The purpose of this study was to assess the characteristics and the biocompatibility of Ti-6Al-4V surface treated by MC. Alpha-beta titanium alloy (Ti-6Al-4V) samples were prepared and they were divided into 6 groups according to chemical concentration and treatment time. These groups include 1) group I, non-treated smooth titanium alloy; 2) group II, MC 1.5 mg/mL for 1 hour; 3) group III, MC 1.5 mg/mL for 24 hours; 4) group IV, MC 15 mg/mL for 10 minutes; 5) group V, MC 100 mg/mL for 5 minutes; 6) group VI, pH1 CA for 3 minutes. The analysis of the surface characteristics of MC-treated titanium alloy was executed using scanning electron microscopy, roughness test, and X-ray photoelectron spectroscopy (XPS). Cell adhesion and MTT assay was done using MC3T3 cell. Titanium surfaces treated with MC indicated a more smoothened surface microstructure. For group II and III, the new peaks of rutile TiO2 were found. Group II and V have more basic group of Ti-OH form in XPS. In MTT assay, all MC-treated groups showed significantly higher cell viability compared to control. The surface roughness, crystal structure, surface hydrophilicity, cell viability of smooth titanium surface was improved by MC treatment. Compared with the control experiment and CA-treated group, smooth titanium surface treated with MC showed improved surface characteristics and cell biocompatibility.

Keywords

References

  1. Gilbert JL. Mechanically assisted corrosion of metallic biomaterials. In: Narayan R, editor. ASM Handbook: Materials for Medical Devices. Ohio: ASM International; 2014. p. 79-89. doi: 10.4012/dmj.26.260.
  2. Yamazoe J, Nakagawa M, Matono Y, Takeuchi A, Ishikawa K. The development of Ti alloys for dental implant with high corrosion resistance and mechanical strength. Dent Mater J 2007;26:260-267. https://doi.org/10.4012/dmj.26.260
  3. Kim YS, Shin SY, Moon SK, Yang SM. Surface properties correlated with the human gingival fibroblasts attachment on various materials for implant abutments: a multiple regression analysis. Acta Odontol Scand 2015;73:38-47. doi: 10.3109/00016357.2014.949845.
  4. Pye AD, Lockhart DE, Dawson MP, Murray CA, Smith AJ. A review of dental implants and infection. J Hosp Infect 2009;72:104-110. doi: 10.1016/j.jhin.2009.02.010.
  5. Berglundh T. Treatment of peri-implant mucositis and peri-implantitis. In: Lindhe J, Lang NP, eds. Clinical periodontology and implant dentistry. West Sussex: John Wiley & Sons; 2015. p. 861-869.
  6. Valderrama P, Blansett JA, Gonzalez MG, Cantu MG, Wilson TG. Detoxification of implant surfaces affected by peri-implant disease: an overview of nonsurgical methods. Open Dent J 2014;8:77-84. doi: 10.2174/1874210601408010077.
  7. Benet LZ, Oie S, Schwartz JB. Design and optimization of dosage regimens; pharmacokinetic data. In: Gilman AG, Goodman LS, Gilman AZ, Hardman JG, Molinoff PB, Limbird LE, editors. Goodman & Gilman's the pharmacological basis of therapeutics. New York: McGraw-Hill; 1996. p. 1707-1792.
  8. Mellonig JT, Griffiths G, Mathys E, Spitznagel J Jr. Treatment of the failing implant: case reports. Int J Periodontics Restorative Dent 1995;15:384-395. doi: 10.11607/prd.00.0133.
  9. Renvert S, Lessem J, Lindahl C, Svensson M. Treatment of incipient peri-implant infections using topical minocycline microspheres versus topical chlorhexidine gel as an adjunct to mechanical debridement. J Int Acad Periodontol 2004;6(4 Suppl):154-159. doi: 10.1111/j.1600-051X.2006.00919.x.
  10. Zhou L, Lin Y, Qiu LX, Chen B, Hu XL, Wang X. Clinical study of periocline on peri-implantitis treatment. Zhonghua Kou Qiang Yi Xue Za Zhi 2006;41:299-303.
  11. Park YJ, Yeom HR, Lee SC, Lee SJ, Chung CP. Drug release kinetics, biodegradability, antimicrobial activity and cytotoxicity of 2% minocycline-loaded polysaccharide microcapsules. J Korean Soc Clin Pharmacol Ther 1995;3:160-168.
  12. Mouhyi J, Sennerby L, Pireaux JJ, Dourov N, Nammour S, Van Reck J. An XPS and SEM evaluation of six chemical and physical techniques for cleaning of contaminated titanium implants. Clin Oral Implants Res 1998;9:185-194. doi: 10.1034/j.1600-0501.1998.090306.x.
  13. Elias CN, Lima JHC, Valiev R, Meyers MA. Biomedical applications of titanium and its alloys. Jom 2008;60:46-49. doi: 10.1007/s11837-008-0031-1.
  14. Klinge B, Hultin M, Berglundh T. Peri-implantitis. Dent Clin North Am 2005;49:661-676. doi: 10.1016/j.cden.2005.03.007.
  15. Lindhe J, Meyle J. Peri-implant diseases: consensus report of the sixth european workshop on periodontology. J Clin Periodontol 2008;35(8 Suppl):282-285. doi: 10.1111/j.1600-051X.2008.01283.x.
  16. Schou S, Holmstrup P, Jorgensen T, Skovgaard LT, Stoltze K, Hjorting-Hansen E, Wenzel A. Implant surface preparation in the surgical treatment of experimental peri-implantitis with autogenous bone graft and ePTFE membrane in cynomolgus monkeys. Clin Oral Implants Res 2003;14:412-422. doi: 10.1034/j.1600-0501.2003.00912.x.
  17. Gosau M, Hahnel S, Schwarz F, Gerlach T, Reichert TE, Burgers R. Effect of six different peri-implantitis disinfection methods on in vivo human oral biofilm. Clin Oral Implants Res 2010;21:866-872. doi: 10.1111/j.1600-0501.2009.01908.x.
  18. Goodson JM. Antimicrobial strategies for treatment of periodontal diseases. Periodontol 2000 1994;5:142-168. doi: 10.1111/j.1600-0757.1994.tb00022.x.
  19. Wheelis SE, Gindri IM, Valderrama P, Wilson TG Jr, Huang J, Rodrigues DC. Effects of decontamination solutions on the surface of titanium: investigation of surface morphology, composition, and roughness. Clin Oral Implants Res 2016;27:329-340. doi: 10.1111/clr.12545.
  20. Suito H, Iwawaki Y, Goto T, Tomotake Y, Ichikawa T. Oral factors affecting titanium elution and corrosion: an in vitro study using simulated body fluid. PLoS One 2013;8:e66052. doi: 10.1371/journal.pone.0066052.
  21. Byun C, Jang JW, Kim IT, Hong KS, Lee BW. Anatase-torutile transition of titania thin films prepared by MOCVD. Mater Res Bull 1997;32:431-440. doi: 10.1016/S0025-5408(96)00203-6.
  22. Jones P, Hockey JA. Infra-red studies of rutile surfaces. Part 2.-hydroxylation, hydration and structure of rutile surfaces. Trans Faraday Soc 1971;67:2679-2685. doi: 10.1039/TF9716702679.
  23. Cui DZ, Park KD, Lee KK, Jung YS, Lee BA, Lee YJ, Kim OS, Chung HJ, Kim YJ. Surface characteristics and osteoblastic cell response to titanium-8tantalum-3neobium alloy. Appl Surf Sci 2012;262:107-109. doi: 10.1016/j.apsusc.2012.02.112.
  24. Wei J, Igarashi T, Okumori N, Igarashi T, Maetani T, Liu B, Yoshinari M. Influence of surface wettability on competitive protein adsorption and initial attachment of osteoblasts. Biomed Mater 2009;4:045002. doi: 10.1088/1748-6041/4/4/045002.
  25. Liao H, Andersson AS, Sutherland D, Petronis S, Kasemo B, Thomsen P. Response of rat osteoblast-like cells to microstructured model surfaces in vitro. Biomaterials 2003;24:649-654. doi: 10.1016/S0142-9612(02)00379-4.

Cited by

  1. Electophoretically Deposition of Ti3C2 on Titanium Surface for Hard Tissue Implant Applications vol.11, pp.7, 2021, https://doi.org/10.3390/coatings11070761