Journal of Forest and Environmental Science

Institute of Forest Science, kangwon National University (강원대학교 산림과학연구소)
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Optimization of Conditions for In Vitro Germination of Seeds of Couroupita guianensis Aubl. - A Threatened Tree with Recalcitrant Seeds

  • M., Manokari (Department of Botany, K.M. Centre for Postgraduate Studies) ;
  • Shekhawat, Mahipal S. (Department of Botany, K.M. Centre for Postgraduate Studies)
  • Received : 2018.08.03
  • Accepted : 2018.08.31
  • Published : 2018.10.31
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Abstract

An efficient and reproducible protocol for in vitro germination of seeds of Couroupita guianensis has been developed in the present study. Couroupita guianensis is a medicinally important tree and categorized as threatened plant species by the IUCN. The seeds were surface sterilized using 0.1% $HgCl_2$ and inoculated on MS (Murashige and Skoog) and Woody Plants (Llyod and McCown) medium. The seeds with partially removed seed coat responded better (100%) on half strength MS medium as compared to the full (51%), one-fourth strength MS (79%) and the WP medium. Half strength MS medium conjunct with $2.0mg{\cdot}L^{-1}$ indole-3 butyric acid (IBA) was found best among the different types of auxins and cytokinins tested for seed germination, as all the seeds germinated on this medium combination. The seedlings were successfully hardened in the greenhouse and restored in the field with 83% survival rate. The protocol can be used as conservation measure for large scale multiplication of this threatened forest tree species.

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References

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