Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Complete Genome of Bacillus subtilis subsp. subtilis KCTC 3135T and Variation in Cell Wall Genes of B. subtilis Strains

  • Ahn, Seonjoo (Department of Biomedical Sciences, Seoul National University College of Medicine) ;
  • Jun, Sangmi (Convergent Research Center for Emerging Virus Infection, Korea Research Institute of Chemical Technology) ;
  • Ro, Hyun-Joo (Convergent Research Center for Emerging Virus Infection, Korea Research Institute of Chemical Technology) ;
  • Kim, Ju Han (Department of Biomedical Sciences, Seoul National University College of Medicine) ;
  • Kim, Seil (Division of Chemical and Medical Metrology, Center for Bioanalysis, Korea Research Institute of Standards and Science)
  • Received : 2017.12.06
  • Accepted : 2018.08.27
  • Published : 2018.10.28
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Abstract

The type strain Bacillus subtilis subsp. subtilis KCTC $3135^T$ was deeply sequenced and annotated, replacing a previous draft genome in this study. The tar and tag genes were involved in synthesizing wall teichoic acids (WTAs), and these genes and their products were previously regarded as the distinguishing difference between B. s. subtilis and B. s. spizizenii. However, a comparative genomic analysis of B. subtilis spp. revealed that both B. s. subtilis and B. s. spizizenii had various types of cell walls. These tar and tag operons were mutually exclusive and the tar genes from B. s. spizizenii were very similar to the genes from non-Bacillus bacteria, unlike the tag genes from B. s. subtilis. The results and previous studies suggest that the tar genes and the tag genes are not inherited after subspecies speciation. The phylogenetic tree based on whole genome sequences showed that each subspecies clearly formed a monophyletic group, while the tree based on tar genes showed that monophyletic groups were formed according to the cell wall type rather than the subspecies. These findings indicate that the tar genes and the presence of ribitol as a cell-wall constituent were not the distinguishing difference between the subspecies of B. subtilis and that the description of subspecies B. s. spizizenii should be updated.

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References

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