Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Antioxidative Activity and Inhibition of Angiotensin Converting Enzyme by Lycii fructus Extracts Prepared by Adding White Ginseng and Red Ginseng

구기자 추출물 제조 시 백삼 및 홍삼 첨가에 의한 항산화활성 및 안지오텐신 전환효소에 대한 저해활성 효과

  • Seong, Bong Jae (Ginseng and Medicinal Plant Research Institute, CNARES) ;
  • Kim, Sun Ick (Ginseng and Medicinal Plant Research Institute, CNARES) ;
  • Jee, Moo Geun (Ginseng and Medicinal Plant Research Institute, CNARES) ;
  • Kim, Soo Dong (Ginseng and Medicinal Plant Research Institute, CNARES) ;
  • Kwon, A Reum (Ginseng and Medicinal Plant Research Institute, CNARES) ;
  • Kim, Hyun Ho (Ginseng and Medicinal Plant Research Institute, CNARES) ;
  • Won, Jun Yeon (Department of Oriental healthcare, Joongbu University) ;
  • Lee, Ka Soon (Ginseng and Medicinal Plant Research Institute, CNARES)
  • 성봉재 (충남농업기술원 인삼약초연구소) ;
  • 김선익 (충남농업기술원 인삼약초연구소) ;
  • 지무근 (충남농업기술원 인삼약초연구소) ;
  • 김수동 (충남농업기술원 인삼약초연구소) ;
  • 권아름 (충남농업기술원 인삼약초연구소) ;
  • 김현호 (충남농업기술원 인삼약초연구소) ;
  • 원준연 (중부대학교 한방건강관리학과) ;
  • 이가순 (충남농업기술원 인삼약초연구소)
  • Received : 2018.07.24
  • Accepted : 2018.09.10
  • Published : 2018.10.30
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Abstract

Background: To enhance the taste and physiological characteristics of Lycii fructus (Gugija) extracts, we investigated the changes in the physiological characteristics of Gugija extracts caused by adding white ginseng (WG) and red ginseng (RG) Methods and Results: Gugija extracts, including 10G10, 10GW-G8 : 2, -G6 : 4, -G4 : 6, -G2 : 8, and -G0 (mixtures made by replacing 0, 20, 40, 60, 80, and 100% of Gugija with WG), as well as 10G10, 10GR-G8 : 2, -G6 : 4, -G4 : 6, -G2 : 8, and -G0 (mixture made by replacing 0, 20, 40, 60, 80, and 100% of Gugija with RG) were extracted with water at 10 times the respective mixture's volume. The antioxidant activities of Gugija extracts were investigated by assessing their 1,1-diphenyl-2-picrydrazyl (DPPH) and 2,2'-azinobis(3ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, ferric reducing antioxidant potential (FRAP) activity, nitrite scavenging activity, and angiotensin converting enzyme (ACE) inhibitory activity. As the amount of WG added increased, the DPPH, and, ABTS radical scavenging activity, and FRAP activity of the Gugija extract decreased. The half maximal inhibitory concentration ($IC_{50}$) value of 10G10, 10GW-G6 : 4, 10GR-G6 : 4, and 10GR-G0 for DPPH radical scavenging activity were $25.50{\pm}1.04$, $52.06{\pm}1.46$, $16.87{\pm}1.24$, and $9.50{\pm}0.16{\mu}{\ell}/m{\ell}$, respectively. On the other hand, the physiological activity of Gugija extract increased with the addition of increasing amounts of RG. However, ACE inhibitory activity was the highest ($50.25{\pm}2.58%$) in the Gugija 10-fold extract without any added RG. Conclusions: From the above results, we suggest that adding RG to Gugija extracts increase their antioxidant, FRAP, and nitrite scavenging activities.

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References

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