Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Morphological and Cytogenetic Analysis of Colchicine-induced Tetraploids of Fallopia multiflolra Haraldson

Colchicine 처리에 의해 유기된 4배체 하수오의 형태 및 세포유전학적 특성

  • Kim, Ki Hyun (Chungbuk Agricultural Research and Extension Services) ;
  • Youn, Cheol Ku (Chungbuk Agricultural Research and Extension Services) ;
  • Kim, In Jae (Chungbuk Agricultural Research and Extension Services) ;
  • Lee, Kyung Ja (Chungbuk Agricultural Research and Extension Services) ;
  • Kim, Young Ho (Chungbuk Agricultural Research and Extension Services) ;
  • Hong, Seong Tack (Chungbuk Agricultural Research and Extension Services) ;
  • Woo, Sun Hee (Department of Crop Science, Chungbuk National University)
  • Received : 2018.08.16
  • Accepted : 2018.10.29
  • Published : 2018.10.30
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Abstract

Background: For stable induction of tetraploidy in Fallopia multiflora Haraldson, colchicine was treated to establish the condition of induction and investigated the morphological and cytogenetic traits of the tetraploid plants obtained compared to those of diploid ones. Methods and Results: For the induction of tetraploidy, F. multiflora plants were soaked in aqueous solutions of colchicine at various concentration (0.1, 0.5, and 1.0%). After this, 2% dimethyl sulfoxide (DMSO) was added at room temperature on a shaker set at 150 rpm for periods of 12, 24, and 48 h. The induction rate of tetraploids appeared to be the highest in plants treated with 0.5% colchicine for 24 h. As the colchicine concentration and soaking time increased above these levels, the growing tip of the roots did not develop and they began to rot. When compared to diploid plants, tetraploids differed greatly in various characteristics, including the sizes and shapes of the leaves, fruits, flowers and roots. The induced tetraploid F. multiflora had larger guard cells, and chloroplasts, increased number of chloroplast in the guard cells and decreased stomatal densities. Conclusions: When colchicine induced plants for tetraploid, it can be distinguished from diploids, in various characteristics such as morphological changes as stomatal size, number of chloroplasts per guard cell, number of chromosomes and flow cytometry. Therefore, it proved that these methods are suitable, quick and easy methods for the identification of the ploidy level of F. multiflora.

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References

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