Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Zinc Increases Chemotactic Activity of Porcine Peripheral Blood Polymorphonuclear Cells

  • Seo, Dong-Hee (Department of Veterinary Medicine, Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University) ;
  • Kang, Byeong-Teck (Department of Veterinary Medicine, Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University) ;
  • Kang, Ji-Houn (Department of Veterinary Medicine, Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University) ;
  • Yang, Mhan-Pyo (Department of Veterinary Medicine, Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University)
  • Received : 2018.07.06
  • Accepted : 2018.10.16
  • Published : 2018.10.31
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Abstract

Zinc is necessary for normal functions in the immune system. The objective of the study is to examine the effect of zinc on the chemotactic activity of porcine peripheral blood polymorphonuclear cells (PMNs). A modified Boyden chamber was used to determine the directional migration distance of PMNs. Various concentrations of zinc showed no chemotactic activity to PMNs. However, culture supernatant from peripheral blood mononuclear cells (PBMCs) treated with zinc remarkably increased the chemotactic activity of PMNs when compared with culture supernatant from PBMCs treated without zinc. Culture supernatant from PBMCs treated without zinc also increased the migration distance of PMNs relative to vehicle control (medium alone). Increasing effect in chemotactic activity of PMNs by culture supernatant from PBMCs treated with zinc was inhibited by treatment of porcine anti-interleukin (IL)-8 polyclonal antibody (pAb). This effect was not affected by heat treatment ($4-85^{\circ}C$). This corresponded with heat stable physical characteristics of IL-8. These results suggest that zinc can upregulate the chemotaxis of PMNs, which is primary mediated by IL-8 chemotactic factor released from PBMCs treated with zinc.

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References

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