Journal of Plant Biotechnology

  • 제45권3호
  • /
  • Pages.236-241
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  • 2018
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  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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Evaluating phylogenetic relationships in the Lilium family using the ITS marker

  • Ghanbari, Sina (Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol) ;
  • Fakheri, Barat Ali (Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol) ;
  • Naghavi, Mohammad Reza (Department of Agronomy and Plant Breeding, College of Agriculture and Natural Resources, University of Tehran) ;
  • Mahdinezhad, Nafiseh (Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol)
  • 투고 : 2018.06.19
  • 심사 : 2018.07.23
  • 발행 : 2018.09.30
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초록

Lilium is a perennial bulbous plant belonging to the liriotypes genus. Our aim was to study the phylogenetic relationships of the Lilium family. Two varieties of Lilium ledebourii, 44 varieties of the gene bank, and one variety from the Tulipa family served as the out group. In order to study the diversity between lilium masses, ITS regions were used to design the marker. The results showed that the guanine base is the most abundant nucleotide. Relatively high conservation was observed in the ITS regions of the populations (0.653). Phylogenetic analysis showed that sargentiae and hybrid varieties are older than other varieties of the Lilium family. Also, the location of L. ledebourii varieties (Damash and Namin) was identified in a phylogenetic tree by using the ITS marker. Overall, our research showed that ITS molecular markers are very suitable for phylogenetic studies in the Lilium family.

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