대한한방내과학회지 (The Journal of Internal Korean Medicine)

대한한방내과학회 (The Society of Internal Korean Medicine)
권호 표지
DOI QR코드

DOI QR Code

Scrophulariae Radix Aqueous Extracts Ameliorate the Pressure Overloaded Heart Failure by Transverse Aortic Constriction in Mice

  • Woo, Seong-jin (Dept. of Cardiovascular and Neurologic Diseases of Korean Internal Medicine, Daegu Oriental Hospital of Daegu Haany University) ;
  • Baek, Kyung-min (Dept. of Cardiovascular and Neurologic Diseases of Korean Internal Medicine, Daegu Oriental Hospital of Daegu Haany University) ;
  • Jang, Woo-seok (Dept. of Cardiovascular and Neurologic Diseases of Korean Internal Medicine, Daegu Oriental Hospital of Daegu Haany University)
  • 투고 : 2018.09.03
  • 심사 : 2018.10.01
  • 발행 : 2018.09.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objectives: The purpose of this study was to investigate the cardioprotective effect of the potent antioxidant properties of Scrophulariae Radix (SR) aqueous extracts by examining pressure overload (PO) heart failure (HF) induced by transverse aortic constriction (TAC) of C57BL/6 mice. Methods: SR (500, 250, 125 mg/kg) and resveratrol (10 mg/kg) were administered orally once a day for 14 days, after the TAC operation. Changes in mortality, the body and heart weights, histopathology of the heart, and antioxidant defense system of the heart were analyzed. Results: After the TAC operation, increases were observed in mortality, heart weights, left ventricular hypertrophy, and lytic and focal fibrotic histological change, and destruction of the heart antioxidant defense system. However, the HF signs showed dose-dependent inhibition following 14 days of continuous oral treatment with SR. A SR dose of 125 mg/kg gave a similar inhibition to that obtained with resveratrol at 10 mg/kg. Conclusions: Oral administration of SR beneficially improves PO-induced HF following TAC surgery by increasing the activity of the heart antioxidant defense system. The overall effect of SR at 125 mg/kg is similar to the effect of resveratrol at 10 mg/kg. However, more detailed mechanistic studies should be performed by screening of the biologically active compounds in SR.

키워드

참고문헌

  1. Juric D, Wojciechowski P, Das DK, Netticadan T. Prevention of concentric hypertrophy and diastolic impairment in aortic-banded rats treated with resveratrol. Am J Physiol Heart Circ Physiol 2007;292(5):H2138-43. https://doi.org/10.1152/ajpheart.00852.2006
  2. Gupta PK, DiPette DJ, Supowit SC. Protective effect of resveratrol against pressure overloadinduced heart failure. Food Sci Nutr 2014;2(3) :218-29. https://doi.org/10.1002/fsn3.92
  3. Movahed A, Yu L, Thandapilly SJ, Louis XL, Netticadan T. Resveratrol protects adult cardiomyocytes against oxidative stress mediated cell injury. Arch Biochem Biophys 2012;527(2):74-80. https://doi.org/10.1016/j.abb.2012.05.002
  4. Qi J, Liu Q, Gong K, Yu J, Wang L, Guo L, et al. Apocynum tablet protects against cardiac hypertrophy via inhibiting AKT and ERK1/2 phosphorylation after pressure overload. Evid Based Complement Alternat Med 2014;2014:769515.
  5. Wojciechowski P, Juric D, Louis XL, Thandapilly SJ, Yu L, Taylor C, et al. Resveratrol arrests and regresses the development of pressure overloadbut not volume overload-induced cardiac hypertrophy in rats. J Nutr 2010;140(5):962-8. https://doi.org/10.3945/jn.109.115006
  6. Thandapilly SJ, Wojciechowski P, Behbahani J, Louis XL, Yu L, Juric D, et al. Resveratrol prevents the development of pathological cardiac hypertrophy and contractile dysfunction in the SHR without lowering blood pressure. Am J Hypertens 2010;23(2):192-6. https://doi.org/10.1038/ajh.2009.228
  7. Xie XY, Xia CS. Advances in studies on chemical constituents and pharmacological activities of Scrophularia ningpoensis Hemsl. Asia Pac Tradit Med 2010;5:121-5.
  8. Díaz AM, Abad MJ, Fernández L, Silván AM, De Santos J, Bermejo P. Phenylpropanoid glycosides from Scrophularia scorodonia: in vitro antiinflammatory activity. Life Sci 2004;74(20):2515-26. https://doi.org/10.1016/j.lfs.2003.10.008
  9. Li J, Huang X, Du X, Sun W, Zhang Y. Study of chemical composition and antimicrobial activity of leaves and roots of Scrophularia ningpoensis. Nat Prod Res 2009;23(8):775-80. https://doi.org/10.1080/14786410802696247
  10. Wang S, Zheng Z, Weng Y, Yu Y, Zhang D, Fan W, et al. Angiogenesis and anti-angiogenesis activity of Chinese medicinal herbal extracts. Life Sci 2004;74(20):2467-78. https://doi.org/10.1016/j.lfs.2003.03.005
  11. Xu FQ, Xu XD, Chen SL. Progress in chemical constituents and bioactivities of Scrophularia ningpoensis. Mod Chin Med 2013;126(15):752-9.
  12. Gu WL, Chen CX, Wu Q, Lü J, Liu Y, Zhang SJ. Effects of Chinese herb medicine Radix Scrophulariae on ventricular remodeling. Pharmazie 2010;65(10):770-5.
  13. Huang XY, Chen CX, Zhang XM, Liu Y, Wu XM, Li YM. Effects of ethanolic extract from Radix Scrophulariae on ventricular remodeling in rats. Phytomedicine 2012;19(3-4):193-205. https://doi.org/10.1016/j.phymed.2011.09.079
  14. Levy D, Larson MG, Vasan RS, Kannel WB, Ho KK. The progression from hypertension to congestive heart failure. JAMA 1996;275(20) :1557-62. https://doi.org/10.1001/jama.1996.03530440037034
  15. Dai DF, Hsieh EJ, Liu Y, Chen T, Beyer RP, Chin MT, et al. Mitochondrial proteome remodelling in pressure overload-induced heart failure: the role of mitochondrial oxidative stress. Cardiovasc Res 2012;93(1):79-88. https://doi.org/10.1093/cvr/cvr274
  16. Griendling KK, FitzGerald GA. Oxidative stress and cardiovascular injury: part II: animal and human studies. Circulation 2003a;108(17):2034-40. https://doi.org/10.1161/01.CIR.0000093661.90582.c4
  17. Griendling KK, FitzGerald GA. Oxidative stress and cardiovascular injury: Part I: basic mechanisms and in vivo monitoring of ROS. Circulation 2003b;108(16):1912-6. https://doi.org/10.1161/01.CIR.0000093660.86242.BB
  18. Crow MT, Mani K, Nam YJ, Kitsis RN. The mitochondrial death pathway and cardiac myocyte apoptosis. Circ Res 2004;95(10):957-70. https://doi.org/10.1161/01.RES.0000148632.35500.d9
  19. van Empel VP, Bertrand AT, van Oort RJ, van der Nagel R, Engelen M, van Rijen HV, et al. EUK-8, a superoxide dismutase and catalase mimetic, reduces cardiac oxidative stress and ameliorates pressure overload-induced heart failure in the harlequin mouse mutant. J Am Coll Cardiol 2006;48(4):824-32. https://doi.org/10.1016/j.jacc.2006.02.075
  20. Comporti M. Lipid peroxidation and cellular damage in toxic liver injury. Lab Invest 1985; 53(6):599-623.
  21. Mansoorali KP, Prakash T, Kotresha D, Prabhu K, Rama Rao N. Cerebroprotective effect of Eclipta alba against global model of cerebral ischemia induced oxidative stress in rats. Phytomedicine 2012;19(12):1108-16. https://doi.org/10.1016/j.phymed.2012.07.004
  22. Koga K, Kenessey A, Ojamaa K. Macrophage migration inhibitory factor antagonizes pressure overload-induced cardiac hypertrophy. Am J Physiol Heart Circ Physiol 2013;304(2):H282-93. https://doi.org/10.1152/ajpheart.00595.2012
  23. Odabasoglu F, Cakir A, Suleyman H, Aslan A, Bayir Y, Halici M, et al. Gastroprotective and antioxidant effects of usnic acid on indomethacin -induced gastric ulcer in rats. J Ethnopharmacol 2006;103(1):59-65. https://doi.org/10.1016/j.jep.2005.06.043
  24. Bento DB, de Souza B, Steckert AV, Dias RO, Leffa DD, Moreno SE, et al. Oxidative stress in mice treated with antileishmanial meglumine antimoniate. Res Vet Sci 2013;95(3):1134-41. https://doi.org/10.1016/j.rvsc.2013.08.004
  25. Cheeseman KH, Slater TF. An introduction to free radical biochemistry. Br Med Bull 1993; 49(3):481-93. https://doi.org/10.1093/oxfordjournals.bmb.a072625
  26. Wagner H, Bauer R, Merlchart D, Xiao PG, Staudinger A. Chromatographic fingerprint analysis of herbal medicines (TLC and HPLC of Chinese Drugs). vol 2. Wien: Springer; 2011, p. 427-37.
  27. Zhang CC, Gu WL, Wu XM, Li YM, Chen CX, Huang XY. Active components from Radix Scrophulariae inhibits the ventricular remodeling induced by hypertension in rats. Springerplus 2016;5:358. https://doi.org/10.1186/s40064-016-1985-z
  28. Kim HS, Park SI, Choi SH, Song CH, Park SJ, Shin YK, et al. Single oral dose toxicity test of blue honeysuckle concentrate in mice. Toxicol Res 2015;31(1):61-8. https://doi.org/10.5487/TR.2015.31.1.061
  29. Lee JS. Schisandrae Fructus administration ameliorates the pressure overload-induced heart failure in mice. Graduate School Daegu Haany University 2014.
  30. Kim SW. Cardioprotective effects of Salviae Milt iorrhiza radix on the pressure overloaded heart failure model by transverse aortic constriction-induced mice. Graduate School Daegu Haany University 2015.
  31. Shin JW. Protective effect of Lonicerae Flos aqueous extracts against pressure overload-induced heart failure model in mice. Daegu Haany University 2016.