DOI QR코드

DOI QR Code

Development of an experimental model for radiation-induced inhibition of cranial bone regeneration

  • Jung, Hong-Moon (Department of Oral and Maxillofacial Surgery, School of Dentistry, Kyungpook National University) ;
  • Lee, Jeong-Eun (Department of Radiation Oncology, School of Medicine, Kyungpook National University) ;
  • Lee, Seoung-Jun (Department of Radiation Oncology, Kyungpook National University Hospital) ;
  • Lee, Jung-Tae (Department of Oral and Maxillofacial Surgery, School of Dentistry, Kyungpook National University) ;
  • Kwon, Tae-Yub (Department of Dental Materials, School of Dentistry, Kyungpook National University) ;
  • Kwon, Tae-Geon (Department of Oral and Maxillofacial Surgery, School of Dentistry, Kyungpook National University)
  • 투고 : 2018.09.03
  • 심사 : 2018.09.20
  • 발행 : 2018.12.31

초록

Background: Radiation therapy is widely employed in the treatment of head and neck cancer. Adverse effects of therapeutic irradiation include delayed bone healing after dental extraction or impaired bone regeneration at the irradiated bony defect. Development of a reliable experimental model may be beneficial to study tissue regeneration in the irradiated field. The current study aimed to develop a relevant animal model of post-radiation cranial bone defect. Methods: A lead shielding block was designed for selective external irradiation of the mouse calvaria. Critical-size calvarial defect was created 2 weeks after the irradiation. The defect was filled with a collagen scaffold, with or without incorporation of bone morphogenetic protein 2 (BMP-2) (1 ㎍/ml). The non-irradiated mice treated with or without BMP-2-included scaffold served as control. Four weeks after the surgery, the specimens were harvested and the degree of bone formation was evaluated by histological and radiographical examinations. Results: BMP-2-treated scaffold yielded significant bone regeneration in the mice calvarial defects. However, a single fraction of external irradiation was observed to eliminate the bone regeneration capacity of the BMP-2-incorporated scaffold without influencing the survival of the animals. Conclusion: The current study established an efficient model for post-radiation cranial bone regeneration and can be applied for evaluating the robust bone formation system using various chemokines or agents in unfavorable, demanding radiation-related bone defect models.

키워드

참고문헌

  1. Dimery IW, Hong WK (1993) Overview of combined modality therapies for head and neck cancer. J Natl Cancer Inst 85:95-111 https://doi.org/10.1093/jnci/85.2.95
  2. Omolehinwa TT, Akintoye SO (2016) Chemical and radiation-associated jaw lesions. Dent Clin N Am 60:265-277 https://doi.org/10.1016/j.cden.2015.08.009
  3. Jegoux F, Malard O, Goyenvalle E, Aguado E, Daculsi G (2010) Radiation effects on bone healing and reconstruction: interpretation of the literature. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 109:173-184 https://doi.org/10.1016/j.tripleo.2009.10.001
  4. van Os R, Thames HD, Konings AW, Down JD (1993) Radiation dose-fractionation and dose-rate relationships for long-term repopulating hemopoietic stem cells in a murine bone marrow transplant model. Radiat Res 136:118-125 https://doi.org/10.2307/3578648
  5. Muller K, Meineke V (2010) Advances in the management of localized radiation injuries. Health Phys 98:843-850 https://doi.org/10.1097/HP.0b013e3181adcba7
  6. Delanian S, Lefaix JL (2004) The radiation-induced fibroatrophic process: therapeutic perspective via the antioxidant pathway. Radiother Oncol 73:119-131 https://doi.org/10.1016/j.radonc.2004.08.021
  7. Kim CM, Park MH, Yun SW, Kim JW (2015) Treatment of pathologic fracture following postoperative radiation therapy: clinical study. Maxillofac Plast Reconstr Surg 37:31 https://doi.org/10.1186/s40902-015-0032-2
  8. Moura LB, Carvalho PH, Xavier CB, Post LK, Torriani MA, Santagata M et al (2016) Autogenous non-vascularized bone graft in segmental mandibular reconstruction: a systematic review. Int J Oral Maxillofac Surg 45:1388-1394 https://doi.org/10.1016/j.ijom.2016.05.004
  9. Kim JW, Hwang JH, Ahn KM (2016) Fibular flap for mandible reconstruction in osteoradionecrosis of the jaw: selection criteria of fibula flap. Maxillofac Plast Reconstr Surg 38:46 https://doi.org/10.1186/s40902-016-0093-x
  10. Paderno A, Piazza C, Bresciani L, Vella R, Nicolai P (2016) Microvascular head and neck reconstruction after (chemo)radiation: facts and prejudices. Curr Opin Otolaryngol Head Neck Surg 24:83-90 https://doi.org/10.1097/MOO.0000000000000243
  11. Kim MG, Lee ST, Park JY, Choi SW (2015) Reconstruction with fibular osteocutaneous free flap in patients with mandibular osteoradionecrosis. Maxillofac Plast Reconstr Surg 37:7 https://doi.org/10.1186/s40902-015-0007-3
  12. Pogrel MA, Podlesh S, Anthony JP, Alexander J (1997) A comparison of vascularized and nonvascularized bone grafts for reconstruction of mandibular continuity defects. J Oral Maxillofac Surg 55:1200-1206 https://doi.org/10.1016/S0278-2391(97)90165-8
  13. Wang Z, Qiu W, Mendenhall WM (2003) Influence of radiation therapy on reconstructive flaps after radical resection of head and neck cancer. Int J Oral Maxillofac Surg 32:35-38 https://doi.org/10.1054/ijom.2002.0320
  14. Beckman JA, Thakore A, Kalinowski BH, Harris JR, Creager MA (2001) Radiation therapy impairs endothelium-dependent vasodilation in humans. J Am Coll Cardiol 37:761-765 https://doi.org/10.1016/S0735-1097(00)01190-6
  15. Wurzler KK, DeWeese TL, Sebald W, Reddi AH (1998) Radiation-induced impairment of bone healing can be overcome by recombinant human bone morphogenetic protein-2. J Craniofac Surg 9:131-137 https://doi.org/10.1097/00001665-199803000-00009
  16. Nussenbaum B, Rutherford RB, Krebsbach PH (2005) Bone regeneration in cranial defects previously treated with radiation. Laryngoscope 115:1170-1177 https://doi.org/10.1097/01.MLG.0000166513.74247.CC
  17. Kinsella CR Jr, Macisaac ZM, Cray JJ, Smith DM, Rottgers SA, Mooney MP et al (2012) Novel animal model of calvarial defect: part III. Reconstruction of an irradiated wound with rhBMP-2. Plast Reconstr Surg 130:643e-650e
  18. Hu WW, Ward BB, Wang Z, Krebsbach PH (2010) Bone regeneration in defects compromised by radiotherapy. J Dent Res 89:77-81 https://doi.org/10.1177/0022034509352151
  19. Woo M, Nordal R (2006) Commissioning and evaluation of a new commercial small rodent x-ray irradiator. Biomed Imaging Interv J 2:e10
  20. Sugiyama K, Yamaguchi M, Kuroda J, Takanashi M, Ishikawa Y, Fujii H et al (2009) Improvement of radiation-induced healing delay by etanercept treatment in rat arteries. Cancer Sci 100:1550-1555 https://doi.org/10.1111/j.1349-7006.2009.01205.x
  21. Espitalier F, Vinatier C, Lerouxel E, Guicheux J, Pilet P, Moreau F et al (2009) A comparison between bone reconstruction following the use of mesenchymal stem cells and total bone marrow in association with calcium phosphate scaffold in irradiated bone. Biomaterials 30:763-769 https://doi.org/10.1016/j.biomaterials.2008.10.051
  22. Kirkby C, Ghasroddashti E, Kovalchuk A, Kolb B, Kovalchuk O (2013) Monte Carlo-based dose reconstruction in a rat model for scattered ionizing radiation investigations. Int J Radiat Biol 89:741-749 https://doi.org/10.3109/09553002.2013.791407
  23. Williams JP, Brown SL, Georges GE, Hauer-Jensen M, Hill RP, Huser AK et al (2010) Animal models for medical countermeasures to radiation exposure. Radiat Res 173:557-578 https://doi.org/10.1667/RR1880.1
  24. Singh VK, Newman VL, Berg AN, MacVittie TJ (2015) Animal models for acute radiation syndrome drug discovery. Expert Opin Drug Discov 10:497-517 https://doi.org/10.1517/17460441.2015.1023290
  25. King MA, Casarett GW, Weber DA (1979) A study of irradiated bone: I. histopathologic and physiologic changes. J Nucl Med 20:1142-1149
  26. Deshpande SS, Donneys A, Farberg AS, Tchanque-Fossuo CN, Felice PA, Buchman SR (2014) Quantification and characterization of radiation-induced changes to mandibular vascularity using micro-computed tomography. Ann Plast Surg 72:100-103 https://doi.org/10.1097/SAP.0b013e318255a57d
  27. Sawada K, Fujioka-Kobayashi M, Kobayashi E, Bromme JO, Schaller B, Miron RJ (2016) In vitro effects of 0 to 120 grays of irradiation on bone viability and release of growth factors. BMC Oral Health 17:4
  28. Doyle JW, Li YQ, Salloum A, FitzGerald TJ, Walton RL (1996) The effects of radiation on neovascularization in a rat model. Plast Reconstr Surg 98:129-135 discussion 36-9 https://doi.org/10.1097/00006534-199607000-00020
  29. Springer IN, Niehoff P, Acil Y, Marget M, Lange A, Warnke PH et al (2008) BMP-2 and bFGF in an irradiated bone model. J Craniomaxillofac Surg 36:210-217 https://doi.org/10.1016/j.jcms.2007.09.001
  30. Ryu SH, Park JH, Jeong ES, Choi SY, Ham SH, Park JI et al (2016) Establishment of a mouse model of 70% lethal dose by total-body irradiation. Lab Anim Res 32:116-121 https://doi.org/10.5625/lar.2016.32.2.116
  31. Rivina L, Schiestl R (2012) Mouse models for efficacy testing of agents against radiation carcinogenesis - a literature review. Int J Environ Res Public Health 10:107-143 https://doi.org/10.3390/ijerph10010107
  32. Wernle JD, Damron TA, Allen MJ, Mann KA (2010) Local irradiation alters bone morphology and increases bone fragility in a mouse model. J Biomech 43:2738-2746 https://doi.org/10.1016/j.jbiomech.2010.06.017

피인용 문헌

  1. Classification of the journal category "oral surgery" in the Scopus and the Science Citation Index Expanded: flaws and suggestions vol.45, pp.4, 2018, https://doi.org/10.5125/jkaoms.2019.45.4.186
  2. The Administration of 4-Hexylresorcinol Accelerates Orthodontic Tooth Movement and Increases the Expression Level of Bone Turnover Markers in Ovariectomized Rats vol.21, pp.4, 2018, https://doi.org/10.3390/ijms21041526
  3. Increased Level of Vascular Endothelial Growth Factors by 4-hexylresorcinol is Mediated by Transforming Growth Factor-β1 and Accelerates Capillary Regeneration in the Burns in Diabetic Animals vol.21, pp.10, 2018, https://doi.org/10.3390/ijms21103473
  4. 방사성골괴사 극복을 위한 피브린지지체의 효용성 평가 vol.15, pp.4, 2018, https://doi.org/10.7742/jksr.2021.15.4.539