Journal of Biomedical and Translational Research

Research Institute of Veterinary Medicine Chungbuk National University (충북대학교 동물의학연구소)
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Screening of immunoactive ingredients in frequently consumed food in Korea

  • Gil, Na-Young (Fermented and Processed Food Science Division, National Institute of Agricultural Sciences, RDA) ;
  • Lee, Sang-Myeong (Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University) ;
  • Mun, Ji-Young (Fermented and Processed Food Science Division, National Institute of Agricultural Sciences, RDA) ;
  • Yeo, Soo-Hwan (Fermented and Processed Food Science Division, National Institute of Agricultural Sciences, RDA) ;
  • Kim, So-Young (Fermented and Processed Food Science Division, National Institute of Agricultural Sciences, RDA)
  • Received : 2018.09.23
  • Accepted : 2018.12.19
  • Published : 2018.12.31
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Abstract

The objectives of this study were to find out the plant to enhance immune activity among 42 kinds of foods frequently consumed by the Korean elderly consisting of 5 food groups and 5 wild plants. Each sample was assessed the immunoactive effect by measuring $NF-{\kappa}B/AP1$ gene expression, nitric oxide and cytokine production in $RAW-Blue^{TM}$ cell. Soybean sprouts of 47 plants showed the highest $NF-{\kappa}B/AP1$ gene expression at the level of $1.13{\pm}0.03$ (O.D. 650 nm) and Soritae, sweet potato, banana, apple, garlic, crown daisy, cabbage and Ailanthus altissima also had high activity of $NF-{\kappa}B/AP1$ gene in $RAW-Blue^{TM}$ cell stimulated by LPS. NO production of Ailanthus altissima was significantly higher than that of other plants and 16 plants of glutinous sorghum, black rice, Seoritae, Heuktae, sweet potato, banana, apple, garlic, mungbean sprouts, spinach, crown daisy, young pumpkin, cabbage, soybean sprouts, Actinidia arguta and Aster scaber were the next best activity. The above results selected 17 out of 47 plant samples. Moreover, soybean sprouts was significantly shown to increase $TNF-{\alpha}$ ($1,509.55{\pm}1.38pg/mL$) and $IL-1{\beta}$ ($54.56{\pm}1.08pg/mL$) cytokines in comparison with RAW-Blue cell stimulated by LPS. According to the results of in vitro evaluation, the ethanol extract of soybean sprout increased the production of immune-enhancing cytokines by proliferation of macrophages. In addition, $NF-{\kappa}B$ transcription factor activity and NO production ability were excellent, and it was selected as a material having excellent immunological activity.

Keywords

Acknowledgement

Supported by : 농촌진흥청

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