The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Isolation and Tyrosinase Inhibitory Activity of Wild Yeasts Obtained from Soil in the Fields of Medicinal Plants, Ginseng and Korean Angelica

인삼과 당귀 재배 토양으로부터 야생효모들의 분리 및 미백성 Tyrosinase 저해활성

  • Kim, Ji-Yoon (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Han, Sang-Min (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Lee, Jong-Soo (Department of Biomedicinal Science and Biotechnology, Paichai University)
  • 김지윤 (배재대학교 바이오.의생명공학과) ;
  • 한상민 (배재대학교 바이오.의생명공학과) ;
  • 이종수 (배재대학교 바이오.의생명공학과)
  • Received : 2018.08.02
  • Accepted : 2018.08.21
  • Published : 2018.09.01
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Abstract

The goal of this study was to isolate wild yeasts from the fields of medicinal plants and investigate its tyrosinase inhibitory activities. Wild yeasts isolated from soil in the ginseng and Korean angelica fields of Geumsan, Chungcheongnam-do, Korea were identified by comparing the nucleotide sequences of the D1/D2 domain of 26S rDNA. In total, 43 yeast strains belonging to 21 species were isolated from 50 soil samples obtained from two medicinal plant fields. From the ginseng field, six strains of Rhodotorula glutinis and four strains of Sampaiozyma ingeniosa were isolated, out of which Rhodotorula glutinis strains were dominant. In the Korean angelica field, six strains of Cyberlindnera saturnus, three strains of Piskurozyma taiwanensis, and three strains of Saitozyma podzolica were isolated, out of which Cyberlindnera saturnus strains were dominant. We prepared cell-free extracts of the isolated wild yeasts and their tyrosinase inhibitory activities were investigated. Among 43 yeast strains, cell-free extracts of Naganishia globosa G1-7 showed the highest tyrosinase inhibitory activity (28.0%).

약용식물 재배지 토양의 야생효모 분포특성을 조사하고 이들 야생효모들의 산업적 응용성을 위한 자료를 얻고자 먼저 약용식물 집단 재배지인 충남 금산의 인삼과 당귀 재배지의 토양들을 2018년 3월에 50점을 채취하여 야생효모들을 분리, 동정하였다. 이들 시료들로부터 21종 43균주의 야생효모들이 분리되었고 인삼재배 논 토양에서는 Rhodotorula glutinis 균주가 6주, 당귀 재배 밭 토양에서는 Cyberlindnera saturnus 균주가 역시 6균주로 가장 많이 분리되었다. 이들 야생효모들을 이용하여 미백성 기능성 화장품 소재를 개발하고자 이들의 무세포추출물을 제조하여 tyrosinase 저해활성을 측정한 결과 저온성 야생효모인 Naganishia globosa G1-7가 28%의 가장 높은 저해활성을 보여 우수효모로 선발하였다.

Keywords

References

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