한국작물학회지 (KOREAN JOURNAL OF CROP SCIENCE)

  • 제63권3호
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  • Pages.257-264
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  • 2018
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  • 0252-9777(pISSN)
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  • 2287-8432(eISSN)
한국작물학회 (The Korean Society of Crop Science)
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콩에서 흡즙해충 싸리수염진딧물 저항성 자원의 발굴

Resistance Resources for the Foxglove Aphid in Soybeans

  • 고홍민 (단국대학교 생명자원과학대학 식량생명공학과) ;
  • 박수민 (단국대학교 생명자원과학대학 식량생명공학과) ;
  • 김경혜 (단국대학교 생명자원과학대학 식량생명공학과) ;
  • 김지민 (단국대학교 생명자원과학대학 식량생명공학과) ;
  • 이택림 (단국대학교 생명자원과학대학 식량생명공학과) ;
  • 허진호 (단국대학교 생명자원과학대학 식량생명공학과) ;
  • 이주석 (한국생명공학연구원 바이오평가센터) ;
  • 정진교 (국립식량과학원 중부작물부) ;
  • 강성택 (단국대학교 생명자원과학대학 식량생명공학과)
  • Koh, Hong-Min (Dept. of Crop Science & Biotechnology, Dankook University) ;
  • Park, Sumin (Dept. of Crop Science & Biotechnology, Dankook University) ;
  • Kim, Kyung Hye (Dept. of Crop Science & Biotechnology, Dankook University) ;
  • Kim, Ji Min (Dept. of Crop Science & Biotechnology, Dankook University) ;
  • Lee, Taeklim (Dept. of Crop Science & Biotechnology, Dankook University) ;
  • Heo, Jinho (Dept. of Crop Science & Biotechnology, Dankook University) ;
  • Lee, Ju Seok (Korea Research Institute of Bioscience & Biotechnology) ;
  • Jung, Jinkyo (National Institute of Crop Science, RDA) ;
  • Kang, Sungteag (Dept. of Crop Science & Biotechnology, Dankook University)
  • 투고 : 2018.08.27
  • 심사 : 2018.09.16
  • 발행 : 2018.09.30
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초록

작물의 병해충 발생은 환경의 여러 가지 요인으로 발생하며 현재 기후의 급격한 변화는 해충류의 급격한 발생빈도 증가를 야기하여 궁극적으로 안정적인 식량 공급의 위협요인이 된다. 본 연구에서는 국내 콩 재배에 있어 많은 피해를 입힐 수 있는 싸리수염진딧물에 대한 저항성 검정지표설정과 저항성 자원발굴을 실시하였다. 기존 콩진딧물에서 사용하는 검정지표는 이동성이 높은 싸리수염진딧물에서 조사의 어려움 있어 비가림온실과 생장상에서의 진딧물 피해 정도를 분석 한 결과를 토대로 싸리수염진딧물 검정지표를 검정한 결과 싸리수염 진딧물은 식물의 피해정도가 안정적으로 나타났고, 간편하게는 접종 14일 후 최초 접종엽의 피해 정도가 싸리수염진딧물 검정지표로 가장 우수하였다. 국내 콩 유전자원의 재배종과 야생종 1,115점의 자원에 대한 Choice test 결과 재배종 23점과 야생종 44점으로 총 67개의 저항성 자원을 선발하였으며 No-Choice test를 통해 재배종에서 8점, 야생종에서 23점으로 항생성을 가진 31개의 유전자원을 선발 할 수 있었다. 선발된 저항성 자원은 생존성 검정결과 선발된 저항성자원에서 진딧물의 생존성이 매우 낮았으며 항생성과 비선호성간에 저항성 반응도 상이함을 확인할 수 있었다. 이상에서 밝혀진 싸리수염저항성 검정지표 및 선발된 자원은 금후 해충저항성 품종개발과 기작구명에 유용하게 활용 가능할 것으로 판단된다.

The recent global climate change induced the drought, flooding, and insect pest outbreaks. These caused the severe damage to crop yield in the domesticated field and occurrence change of insect pest species. The sap-sucking insect pest, aphids are common in soybean [Glycine max (L.) Merr.] and cause serious yield losses. Thus, developing resistance cultivars is promising and efficient strategy to prevent the significant yield losses by aphid and screening germplasm is the essential procedure to achieve this goal. We tried to establish a resistance test indicator for foxglove aphid, Aulacorthum solani (Kaltenbach), in soybean and found that plant damage degree or infested plant damage is most suitable one. Also we screened around 1,200 of soybean germplasm including wild and cultivated species for its resistance to foxglove aphid from the various origins, and 67 soybeans, including PI 366121, showed antixenosis resistance, 31 germplasms among 67 antixenosis germplasms were showed antibiosis to foxglove aphid with non-choice test. The identified foxglove aphid resistant soybean resources showed significantly low rate in survival test. Furthermore, resistance type, (i.e., antibiosis or antixenosis) of each candidate were varied. In this research, we established the screening index for foxglove aphid resistance in soybean, and identified the resistance varieties. This result could be useful resources in breeding for new foxglove aphid resistance soybean cultivars, and provide fundamental information to investigate the resistance mechanism in soybean.

키워드

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