Korean Journal of Otorhinolaryngology-Head and Neck Surgery

Korean Society of Otolaryngology-Head and Neck Surgery (대한이비인후과학회)
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Triptolide Inhibits Lipopolysaccharide-Induced MUC5AC/5B Expression via Nuclear Factor-Kappa B in Human Airway Epithelial Cells

사람 호흡기 상피세포에서 Triptolide의 Nuclear Factor-Kappa B를 통한 Lipopolysaccharide로 유도된 MUC5AC/5B 발현 억제 효과

  • Seo, Bo Hyeon (Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Yeungnam University) ;
  • Choi, Tae Yeong (Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Yeungnam University) ;
  • Choi, Yoon Seok (Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Yeungnam University) ;
  • Bae, Chang Hoon (Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Yeungnam University) ;
  • Na, Hyung Gyun (Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Yeungnam University) ;
  • Song, Si-Youn (Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Yeungnam University) ;
  • Kim, Yong-Dae (Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Yeungnam University)
  • 서보현 (영남대학교 의과대학 이비인후-두경부외과학교실) ;
  • 최태영 (영남대학교 의과대학 이비인후-두경부외과학교실) ;
  • 최윤석 (영남대학교 의과대학 이비인후-두경부외과학교실) ;
  • 배창훈 (영남대학교 의과대학 이비인후-두경부외과학교실) ;
  • 나형균 (영남대학교 의과대학 이비인후-두경부외과학교실) ;
  • 송시연 (영남대학교 의과대학 이비인후-두경부외과학교실) ;
  • 김용대 (영남대학교 의과대학 이비인후-두경부외과학교실)
  • Received : 2018.03.27
  • Accepted : 2018.06.28
  • Published : 2018.12.25
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Abstract

Background and Objectives The representative mucin genes in the human airway are MUC5AC and MUC5B, which are regulated by several inflammatory and anti-inflammatory substances. Triptolide (TPL), udenafil, betulinic acid, changkil saponin, and glucosteroid are some of the many anti-inflammatory substances that exist. TPL is a diterpenoid compound from the thunder god vine, which is used in traditional Chinese medicine for treatment of immune inflammatory diseases, such as rheumatoid arthritis, systemic lupus erythematosus, nephritis and asthma. However, the effects of TPL on mucin expression of human airway epithelial cells have yet to be reported. Hence, this study investigated the effect of TPL on lipopolysaccharide (LPS)-induced MUC5AC and MUC5B expression in human airway epithelial cells. Subjects and Method The NCI-H292 cells and the primary cultures of human nasal epithelial cells were used to investigate the effects of TPL on LPS-induced MUC5AC and MUC5B expression using real-time polymerase chain reaction, enzyme immunoassay, and Western blot. Results TPL significantly decreased the LPS-induced MUC5AC and MUC5B mRNA expression and protein production. TPL also significantly decreased the nuclear factor-kappa B (NF-kB) phosphorylation. Conclusion These results suggest that TPL down regulates MUC5AC and MUC5B expression via inhibition of NF-kB activation in human airway epithelial cells. This study may provide important information about the biological role of triptolide on mucus-secretion in airway inflammatory diseases and the development of novel therapeutic agents for controlling such diseases.

Keywords

References

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