Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Analysis of sex ratio on bovine in vitro fertilized embryos using sex determination kit treated sperm

성분리 키트가 처리된 소정자를 이용한 체외수정란의 배양과 성분리 효율

  • Heo, Young-Tae (Department of Animal Science and Biotechnology, Sangji Youngseo College) ;
  • Kim, Dong-Gu (Nuri Science) ;
  • Uhm, Sang-jun (Department of Animal Science and Biotechnology, Sangji Youngseo College)
  • 허영태 (상지영서대학교 동물생명산업과) ;
  • 김동구 ((주) 누리사이언스) ;
  • 엄상준 (상지영서대학교 동물생명산업과)
  • Received : 2018.08.23
  • Accepted : 2018.09.27
  • Published : 2018.09.30
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Abstract

It has been claimed that artificial insemination (AI) of cows with frozen-thawed semen treated with commercially produced kits, Wholemom (in favour of female gender) increases the birth chance of calves with desired sex ratio by approximately 85% without decrease of pregnancy rates. Hence, this study was conducted to investigate the efficacy of wholemom kits as combined with frozen-thawed bovine semen during in vitro fertilization on the in vitro fertilization and developmental efficiency and sex ratios such as some reproductive parameters in bovine. For this, 1,737 oocytes were in vitro fertilized and developed. Agglutination effects on bovine after treatment of Wholemom kit were observed by time passage and dose respectively. To determine sex of embryos, Bovine embryo Y-specific gene primers(ConEY) and Bovine specific universal primer(ConBV) were used as multiple PCR method. Fertilization rate of wholemom-treated group was significantly lower than its of control group[66.9% (1,156/1,737) in Wholemom-treated group; 75.0% (610/813) in control group]. However, developmental rate after fertilization of both wholemom-treated and control groups were not significantly different [26.1% (404/1,156) in Wholemom-treated group; 27.4% (224/610) in control group]. Sex ratio of in vitro fertilized embryo with frozen-thawed semen treated with wholemom kit was determined by multi PCR. Female ratio in wholemom-treated group [85.4% (173/201)] was significantly higher than its of control group [47.2% (66/141)]. In conclusion, wholemom treatments of semen used in the in vitro fertilization and development of bovine oocytes provided increase in female ratio with decrease of fertilization rate.

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References

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