한국수정란이식학회지 (Journal of Embryo Transfer)

  • 제33권3호
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  • Pages.139-147
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  • 2018
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  • 2508-755X(pISSN)
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  • 2288-0178(eISSN)
한국수정란이식학회 (The Korean Society of Embryo Transfer)
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개 parthenote in vitro culture시 EDTA 첨가에 의한 발달율 향상

Effect of EDTA on canine parthenote development during in vitro culture

  • 정해윤 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • ;
  • 노진구 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • ;
  • 이휘철 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 위하연 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 옥선아 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 우제석 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 허태영 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 임기순 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 김종국 (전북대학교 농업생명과학대학 가축번식학 연구실) ;
  • 이승훈 (농촌진흥청 국립축산과학원 동물바이오공학과)
  • Jeong, Haeyun (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Zhao, Minghui (Qingdao Agricultural University) ;
  • No, Jin-Gu (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Ullah, Imran (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Lee, Whi-Cheul (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Wi, Hayeon (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Ock, Sun A (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Hur, Tai-young (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Woo, Jae-Seok (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Im, Gi-sun (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Jong-Gug (Animal Reproduction Laboratory, College of Agriculture and Life Science, Chonbuk National University) ;
  • Lee, Seunghoon (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration)
  • 투고 : 2018.09.03
  • 심사 : 2018.09.21
  • 발행 : 2018.09.30
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초록

Somatic cell nuclear transfer (SCNT) is a useful biotechnological tool for animal cloning. Until now, SCNT has been inefficient, especially in dog. It is believed that an embryo developmental block in SCNT embryos is cause of low production efficiency. However, no studies have been performed on canines for embryo developmental block. In this study, we attempted to evaluate the beneficial role of EDTA in canine parthenogenic (PA) embryos development to overcome embryo developmental block. The PA embryos were divided into 0.01 mM EDTA treated and non-treated groups. Embryo developmental efficiency was measured by activating chemically parthenote. After EDTA induction, PA embryos were evaluated for embryonic development, Reactive Oxygen Species (ROS) activity, mitochondrial integrity, ATP production and genomic activation. The EDTA treated PA embryos showed significantly higher survival rate and improved cavity formation compared to non-treated. Furthermore, cytoplasmic ROS level was mitigated and mitochondrial membrane potential was found significantly higher in EDTA treated group followed by higher ATP production. Moreover, major embryonic genomic activation specific markers/factors were also elevated in EDTA treated group. Conclusively, we elucidated that EDTA showed substantially positive effect to overcome embryo developmental block in canine.

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참고문헌

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