한국버섯학회지 (Journal of Mushroom)

  • 제16권3호
  • /
  • Pages.170-174
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  • 2018
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  • 1738-0294(pISSN)
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  • 2288-8853(eISSN)
한국버섯학회 (The Korean Society of Mushroom Science)
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표고버섯 수확 후 배지추출물의 인삼잿빛곰팡이 병 방제 효과

Protective effects of extracts from spent mushroom substrate of Lentinula edodes on gray mold disease of ginseng

  • 유혜린 (한경대학교 미래융합기술대학원) ;
  • 김재경 (한경대학교원예생명과학과) ;
  • 조진주 (한경대학교원예생명과학과) ;
  • 강희완 (한경대학교원예생명과학과)
  • Lyu, Hae-lin (Graduate School of Future Convergence Technology, Hankyong National University) ;
  • Kim, Jae-Kyong (Department of Horticultural life Science, Hankyong National University) ;
  • Cho, Jin-Joo (Department of Horticultural life Science, Hankyong National University) ;
  • Kang, Hee-Wan (Department of Horticultural life Science, Hankyong National University)
  • 투고 : 2018.08.31
  • 심사 : 2018.09.17
  • 발행 : 2018.09.30
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초록

본 연구는 표고 수확 후 배지의 물추출물(WESMS)과 70% 메탄올 추출물(MeOHSMS)의 인삼잿빛곰팡이병원균에대한 항균활성과 병 방제효과를 조사하기 위하여 수행 되었다. WESMS와 MeOHSMS는 35%와 75%의 균사생장 억제율을 보였으며 MeOHSMS는 포자발아를 95% 이상 억제시켰다. WESMS와 MeOHSMS는 병 발병정도가 1.2와 0.7로서 대조구 4.5보다 현저히 낮게 나타났으며 80% 이상의 방제효과를 보였다. MeOHSMS 처리시 인삼잎에서 페놀함량이 28.0 mg GAE/100 g로 물 처리구 18.0 mg GAE/100g과 BABA 처리구 23 mg GAE/100g 보다 페놀함량이 18%에서 35% 증가되었다.

This study aims to investigate the antifungal and protective effects of water- and 70% methyl alcohol-extracts from spent mushroom substrate (WESMS and MeOHSMS) of Lentinula edodes, on Botrytis cinerea- the causative agent for gray mold disease in ginseng. MeOHSMS inhibited mycelial growth and spore germination of Botrytis cinerea, by 75% and 95%, respectively. MeOHSMS could suppress gray mold disease of ginseng seedlings by 80% and effectively reduce the disease severity by 60%. Compared to the treatment of ginseng leaves with WESMS and DL-${\beta}$-aminobutyric acid (BABA), the MeOHSMS treatment increased the phenolic compounds in the leaves by 36% and 18%, respectively. These results suggest that the SMS extracts suppress gray mold disease in ginseng via dual functions: antifungal activity and increase in a plant defense factor-phenolic compounds.

키워드

참고문헌

  1. Agrios GN. 2005. Plant Pathology. Elservier Academic Press, San Diego, California, USA. pp 77-122.
  2. Alves MJ, Ferreira ICFR, Joana Dias J, Teixeira V, Martins A, Pintado M1A. 2012. Review on Antimicrobial Activity of Mushroom (Basidiomycetes) Extracts and Isolated Compounds. Planta Med 78:1707-1718. https://doi.org/10.1055/s-0032-1315370
  3. Chen JT, Huang JW. 2010. Antimicrobial activity of edible mushroom culture filtrates on plant pathogens. Plant Pathol Bulletin 19:261-270.
  4. Hautzel R, Anke T. 1990. Screening of basidiomycetes and ascomycetes for plant growth regulating substances. Introduction of the gibberellic acid induced de-novo synthesis of hydrolytic enzymes in embryoless seeds of Triticum aestivum as test system. Z Naturforsch 45:1093-1098.
  5. Kang DS, Min KJ, Kwak AM, Lee SY, Kang HW. 2017. Defense response and suppression of Phytophthora Blight disease of pepper by water extract from spent mushroom substrate of Lentinula edodes. Plant Pathol J 33:264-275. https://doi.org/10.5423/PPJ.OA.02.2017.0030
  6. Kwak AM, Lee SY, Kang, HW. 2016. Suppression of water extract from spent mushroom substrates of Pleurotus eryngii against tomato bacterial wilt disease. Kor J Mycol 44:123-329.
  7. Kim JH, Kim GH, Kim HT. 2011. Sensitive of Botrytis cinerea isolated from infected leaves of Ginseng to Tolyfluanid. Kor J Pesticide Sci 15:188-193.
  8. Lim SH, Lee YH, Kang HW. 2013. Optimal extraction and characteristics of lignocellulytic enzymes from various spent mushroom composts. Mycobiology 41:160-166.
  9. Mikulic-Petkovsek M, Schmitzer V, Jakopic J, Cunja V, Veberic R, Munda A, Stampar F. 2013. Phenolic compounds as defence response of pepper fruits to Colletotrichum coccodes. Physiol Mol Plant Pathol 84:138-145. https://doi.org/10.1016/j.pmpp.2013.09.003
  10. Ministry of Agriculture, Food and Rural Affairs. 2016. A collection of ginseng statistics. pp 32. Korea
  11. Parada RY, Murakami S, Shimomura N, Egusa M, Otani H. 2011. Autoclaved spent substrate of hatakeshimeji mushroom (Lyophyllum decastes Sing.) and its water extract protect cucumber from anthracnose. Crop Protection 443-450.
  12. Parada RY, Murakami S, Shimomura N, Otani, H. 2012. Suppression of fungal and bacterial diseases of cucumber plants by using the spent mushroom substrate of Lyophyllum decastes and Pleurotus eryngii. J Phytopathol 160:390-396. https://doi.org/10.1111/j.1439-0434.2012.01916.x
  13. Suay I, Arenal F, Asensio FJ, Basilio A, Cabello MA, Díez MT, Garcia JB, Va AG, Gorrochategui J, Hernandez P, Pelaez F, Vicente MF. 2000. Screening of basidiomycetes for antimicrobial activities. Antonie Leeuwenhoek 78:129-139. https://doi.org/10.1023/A:1026552024021
  14. Subramaniyam R, Vimala R. 2012. Solid state and submerged fermentation for the production of bioactive substances: a comparative study. Int J Sci Nat 3:480-486.
  15. Suess A, 2006. Report: Value-Added Strategies for Spent Mushroom Substrate in BC. British Columbia Ministry of Agriculture and Lands. pp 1-101.
  16. Sunwoo JY, Lee YK, Hwang BK. 1996. Induced resistance against Phytophthora capsici in pepper plants in response to $DL-{\beta}- amino-n-butyric$ acid. Eur J Plant Pathol 102: 663-670. https://doi.org/10.1007/BF01877247
  17. Yohalem DS, Nordheim EV, Andrews JH. 1996. The effect of water extracts of spent mushroom compost on aple scab in the field. Phytopathology 86:914-922. https://doi.org/10.1094/Phyto-86-914