생약학회지 (Korean Journal of Pharmacognosy)

  • 제49권3호
  • /
  • Pages.231-239
  • /
  • 2018
  • /
  • 0253-3073(pISSN)
  • /
  • 2288-9299(eISSN)
한국생약학회 (The Korean Society of Pharmacognosy)
권호 표지

1-methyl-4-phenylpyridinium으로 유도된 신경 손상에 대한 호두나무잎에서 분리된 Hyperoside의 보호 효과

Protective Effects of Hyperoside from Juglans sinensis Leaves against 1-methyl-4-phenylpyridinium-Induced Neurotoxicity

  • Pariyar, Ramesh (Institute of Pharmaceutical Research and Development,College of Pharmacy, Wonkwang University) ;
  • Svay, Thida (Institute of Pharmaceutical Research and Development,College of Pharmacy, Wonkwang University) ;
  • Seo, Jungwon (Institute of Pharmaceutical Research and Development,College of Pharmacy, Wonkwang University)
  • 투고 : 2018.09.10
  • 심사 : 2018.09.19
  • 발행 : 2018.09.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Parkinson's disease (PD), one of common neurodegenerative diseases, is caused by the death of dopaminergic neurons in the substantia nigra pars compacta. The loss of dopaminergic neurons in PD is associated with oxidative stress and mitochondrial dysfunction. Hyperoside (quercetin 3-O-${\beta}$-D-galactopyranoside) was reported to have protective properties against oxidative stress by reducing intracellular reactive oxygen species (ROS) and increasing antioxidant enzyme activity. In this study, we examined the neuroprotective effect of hyperoside against 1-methyl-4-phenyl pyridinium ($MPP^+$)-induced cell model of PD and the underlying molecular mechanisms. Hyperoside significantly decreased $MPP^+$-induced cell death, accompanied by a reduction in poly ADP-ribose polymerase (PARP) cleavage. Furthermore, it attenuated $MPP^+$-induced intracellular ROS and disruption of mitochondrial membrane potential (MMP), with the reduction of Bax/Bcl-2 ratio. Moreover, hyperoside significantly increased the phosphorylation of Akt, but it has no effects on $GSK3{\beta}$ and MAPKs. Pharmacological inhibitor of PI3K/Akt abolished the cytoprotective effects of hyperoside against $MPP^+$. Taken together, these results demonstrate that hyperoside significantly attenuates $MPP^+$-induced neurotoxicity through PI3K/Akt signaling pathways in SH-SY5Y cells. Our findings suggest that hyperoside might be one of the potential candidates for the treatment of PD.

키워드

참고문헌

  1. Fearnley, J. M. and Lees, A. J. (1991) Ageing and Parkinson's disease: substantia nigra regional selectivity. Brain 114: 2283-2301. https://doi.org/10.1093/brain/114.5.2283
  2. Wang, Y., Gao, J., Miao, Y., Cui, Q., Zhao, W., Zhang, J. and Wang, H. (2014) Pinocembrin protects SH-SY5Y cells against MPP+-induced neurotoxicity through the mitochondrial apoptotic pathway. J. Mol Neurosci. 53: 537-545. https://doi.org/10.1007/s12031-013-0219-x
  3. Petit-Paitel, A., Brau, F., Cazareth, J. and Chabry, J. (2009) Involvment of cytosolic and mitochondrial GSK-$3{\beta}$ in mitochondrial dysfunction and neuronal cell death of MPTP/MPP+-treated neurons. PloS one 4: e5491. https://doi.org/10.1371/journal.pone.0005491
  4. Itano, Y. and Nomura, Y. (1995) 1-methyl-4-phenyl-pyridinium ion (MPP+) causes DNA fragmentation and increases the Bcl-2 expression in human neuroblastoma, SH-SY5Y cells, through different mechanisms. Brain Res. 704: 240-245. https://doi.org/10.1016/0006-8993(95)01120-X
  5. Lu, S., Lu, C., Han, Q., Li, J., Du, Z., Liao, L. and Zhao, R. C. (2011) Adipose-derived mesenchymal stem cells protect PC12 cells from glutamate excitotoxicity-induced apoptosis by upregulation of XIAP through PI3-K/Akt activation. Toxicology 279: 189-195. https://doi.org/10.1016/j.tox.2010.10.011
  6. Lin, Y. L., Wang, G. J., Huang, C. L., Lee, Y. C., Liao, W. C., Lai, W. L., Lin, Y. J. and Huang, N. K. (2009) Ligusticum chuanxiong as a potential neuroprotectant for preventing serum deprivation-induced apoptosis in rat pheochromocytoma cells: functional roles of mitogen-activated protein kinases. J. Ethnopharmacol. 122: 417-423. https://doi.org/10.1016/j.jep.2009.02.011
  7. Liu, Y. H., Liu, G. H., Mei, J. J. and Wang, J. (2016) The preventive effects of hyperoside on lung cancer in vitro by inducing apoptosis and inhibiting proliferation through Caspase-3 and P53 signaling pathway. Biomed. Pharmacother. 83: 381-391. https://doi.org/10.1016/j.biopha.2016.06.035
  8. Kim, S.-J., Um, J.-Y., Hong, S.-H. and Lee, J.-Y. (2011) Anti-inflammatory activity of hyperoside through the suppression of nuclear factor-${\kappa}B$ activation in mouse peritoneal macrophages. Am. J. Chin. Med. 39: 171-181. https://doi.org/10.1142/S0192415X11008737
  9. Piao, M. J., Kang, K. A., Zhang, R., Ko, D. O., Wang, Z. H., You, H. J., Kim, H. S., Kim, J. S., Kang, S. S. and Hyun, J. W. (2008) Hyperoside prevents oxidative damage induced by hydrogen peroxide in lung fibroblast cells via an antioxidant effect. Biochim. Biophys. Acta. 1780: 1448-1457. https://doi.org/10.1016/j.bbagen.2008.07.012
  10. Zheng, M., Liu, C., Pan, F., Shi, D. and Zhang, Y. (2012) Antidepressant-like effect of hyperoside isolated from Apocynum venetum leaves: possible cellular mechanisms. Phytomedicine 19: 145-149. https://doi.org/10.1016/j.phymed.2011.06.029
  11. Hu, J., Wang, Z., Guo, Y. Y., Zhang, X. N., Xu, Z. H., Liu, S. B., Guo, H. J., Yang, Q., Zhang, F. X., Sun, X. L. and Zhao, M. G. (2009) A role of periaqueductal grey NR2B-containing NMDA receptor in mediating persistent inflammatory pain. Mol. Pain 5: 71.
  12. Wang, W. Q., Ma, C. G. and Xu, S. Y. (1996) Protective effect of hyperin against myocardial ischemia and reperfusion injury. Zhongguo. Yao. Li. Xue. Bao. 17: 341-344.
  13. Yuning, G. L. Y. M. Y. (2001) Study of Cuscuta australis hyperoside on immunological function of mice in vivo and in vitro. Chinese Journal of Information on TCM. 11: 027.
  14. Zeng, K. W., Wang, X. M., Ko, H., Kwon, H. C., Cha, J. W. and Yang, H. O. (2011) Hyperoside protects primary rat cortical neurons from neurotoxicity induced by amyloid beta-protein via the PI3K/Akt/Bad/Bcl(XL)-regulated mitochondrial apoptotic pathway. Eur. J. Pharmacol. 672: 45-55. https://doi.org/10.1016/j.ejphar.2011.09.177
  15. Liu, Z., Tao, X., Zhang, C., Lu, Y. and Wei, D. (2005) Protective effects of hyperoside (quercetin-3-o-galactoside) to PC12 cells against cytotoxicity induced by hydrogen peroxide and tert-butyl hydroperoxide. Biomed. Pharmacother. 59: 481-490. https://doi.org/10.1016/j.biopha.2005.06.009
  16. Chen, Z., Ma, C. and Zhao, W. (1998) Protective effect of hyperin against cerebral ischemia-reperfusion injury. Acta. Pharm. Sin. 33: 14-17.
  17. Chen, Z., Zhang, J. and Ma, C. (1998) Protective effect of hyperin on cerebral infarction in rats. Zhongguo. Zhong. Yao. Za. Zhi. 23: 626-628, inside back cover.
  18. Liu, R. L., Xiong, Q. J., Shu, Q., Wu, W. N., Cheng, J., Fu, H., Wang, F., Chen, J. G. and Hu, Z. L. (2012) Hyperoside protects cortical neurons from oxygen-glucose deprivation-reperfusion induced injury via nitric oxide signal pathway. Brain Res. 1469: 164-173. https://doi.org/10.1016/j.brainres.2012.06.044
  19. An, R. B., Kim, H. C., Tian, Y. H. and Kim, Y. C. (2005) Free radical scavenging and hepatoprotective constituents from the leaves of Juglans sinensis. Arch. Pharm. Res. 28: 529-533. https://doi.org/10.1007/BF02977753
  20. Zhu, J. H., Horbinski, C., Guo, F., Watkins, S., Uchiyama, Y. and Chu, C. T. (2007) Regulation of autophagy by extracellular signal-regulated protein kinases during 1-methyl-4-phenylpyridinium-induced cell death. Am. J. Pathol. 170: 75-86. https://doi.org/10.2353/ajpath.2007.060524
  21. Chun, H. S., Gibson, G. E., DeGiorgio, L. A., Zhang, H., Kidd, V. J. and Son, J. H. (2001) Dopaminergic cell death induced by MPP(+), oxidant and specific neurotoxicants shares the common molecular mechanism. J. Neurochem. 76: 1010-1021. https://doi.org/10.1046/j.1471-4159.2001.00096.x
  22. Blesa, J., Trigo-Damas, I., Quiroga-Varela, A. and Jackson-Lewis, V. R. (2015) Oxidative stress and Parkinson's disease. Front. Neuroanat. 9: 91.
  23. Chan, P. H. (2004) Mitochondria and neuronal death/survival signaling pathways in cerebral ischemia. Neurochem. Res. 29: 1943-1949. https://doi.org/10.1007/s11064-004-6869-x
  24. Cassarino, D. S., Parks, J. K., Parker, W. D., Jr. and Bennett, J. P., Jr. (1999) The parkinsonian neurotoxin MPP+ opens the mitochondrial permeability transition pore and releases cytochrome c in isolated mitochondria via an oxidative mechanism. Biochim. Biophys. Acta. 1453: 49-62. https://doi.org/10.1016/S0925-4439(98)00083-0
  25. Fernandes-Alnemri, T., Litwack, G. and Alnemri, E. S. (1994) CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme. J. Biol. Chem. 269: 30761-30764.
  26. Oliver, F. J., de la Rubia, G., Rolli, V., Ruiz-Ruiz, M. C., de Murcia, G. and Mnissier-de Murcia, J. (1998) Importance of poly (ADP-ribose) polymerase and its cleavage in apoptosis Lesson from an uncleavable mutant. J. Biol. Chem. 273: 33533-33539. https://doi.org/10.1074/jbc.273.50.33533
  27. Pu, X., Song, Z., Li, Y., Tu, P. and Li, H. (2003) Acteoside from Cistanche salsa inhibits apoptosis by 1-methyl-4-phenylpyridinium ion in cerebellar granule neurons. Planta Med. 69: 65-66. https://doi.org/10.1055/s-2003-37029
  28. Zhang, C., Yuan, X., Hu, Z., Liu, S., Li, H., Wu, M., Yuan, J., Zhao, Z., Su, J. and Wang, X. (2017) Valproic acid protects primary dopamine neurons from MPP+-Induced Neurotoxicity: Involvement of $GSK3{\beta}$ Phosphorylation by Akt and ERK through the Mitochondrial Intrinsic Apoptotic Pathway. Biomed. Res. Int. 2017: 12.
  29. Weng, Z., Signore, A. P., Gao, Y., Wang, S., Zhang, F., Hastings, T., Yin, X. M. and Chen, J. (2007) Leptin protects against 6-hydroxydopamine-induced dopaminergic cell death via mitogen-activated protein kinase signaling. J. Biol. Chem. 282: 34479-34491. https://doi.org/10.1074/jbc.M705426200
  30. Zhao, Q., Ye, J., Wei, N., Fong, C. and Dong, X. (2016) Protection against MPP(+)-induced neurotoxicity in SH-SY5Y cells by tormentic acid via the activation of PI3-K/Akt/GSK3beta pathway. Neurochem. Int. 97: 117-123. https://doi.org/10.1016/j.neuint.2016.03.010
  31. Datta, S. R., Dudek, H., Tao, X., Masters, S., Fu, H., Gotoh, Y. and Greenberg, M. E. (1997) Akt phosphorylation of BAD couples survival signals to the cell-intrinsic death machinery. Cell 91: 231-241. https://doi.org/10.1016/S0092-8674(00)80405-5