Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Stimulation of the milk protein production in MAC-T cells by purified bee venom

정제봉독의 MAC-T 세포에서 유단백 합성 촉진효과

  • Han, Sang-Mi (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration) ;
  • Woo, Soon-Ok (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration) ;
  • Kim, Se-Gun (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration) ;
  • Jang, Hye-Ri (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration)
  • 한상미 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 우순옥 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 김세건 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 장혜리 (농촌진흥청 국립농업과학원 농업생물부)
  • Received : 2018.07.19
  • Accepted : 2018.09.21
  • Published : 2018.09.30
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Abstract

Purified bee venom was collected from colonies of honeybees (Apis mellifera L.) using a bee venom collector under sterile conditions and then purified under strict laboratory conditions. Purified bee venom contained $63.9{\pm}5.4%$ melittin, $10.9{\pm}1.6%$ phospholipase A2, and $2.3{\pm}0.3%$ apamin. Purified bee venom has various anti-bacterial, anti-inflammatory and immunostimulating effects. In this study, we evaluated purified bee venom which are mammary gland cells, MAC-T cells are used to increase the synthesis of milk protein. Purified bee venom promoted the proliferation of MAC-T cells at concentrations below $1{\mu}g/mL$, but cytotoxicity at $10{\mu}g/mL$ and above. As a result of the increase in the synthesis of ${\beta}-casein$, a milk protein after treatment with MAC-T cells at a concentration of the bee venom without cytotoxicity, the ${\beta}-casein$ content in the cell culture was increased when treated at a concentration of 1 ng/mL or more. In addition, it was confirmed that purified bee venom significantly increased the expression of bovine ${\beta}-casein$ (bCSNB) mRNA, a ${\beta}-casein$ synthesis gene, at a concentration of 1 ng/mL or more. These results suggest that purified bee venom can be used to increase the production of livestock by ultimately increasing the expression of milk protein.

Keywords

References

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