Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Analysis of virulence gene profiles of Salmonella spp. and Enterococcus faecalis isolated from the freshly slaughtered poultry meats produced in Gyeong-Nam province

경남지역 가금류 도축장 신선육에서 분리한 Salmonella spp.와 Enterococcus faecalis의 독성인자 보유 패턴 분석

  • 하도윤 (경상남도 동물위생시험소) ;
  • 차휘근 (경상남도 동물위생시험소) ;
  • 한권식 (경상남도 동물위생시험소) ;
  • 장은희 (경상남도 동물위생시험소) ;
  • 박하영 (경상남도 동물위생시험소) ;
  • 배민진 (경상남도 동물위생시험소) ;
  • 조아름송이 (경상남도 동물위생시험소) ;
  • 이후근 (경상남도 동물위생시험소) ;
  • 고병효 (경상남도 동물위생시험소) ;
  • 김도경 (경상남도 동물위생시험소) ;
  • 황보원 (경상남도 동물위생시험소) ;
  • 김상현 (경상대학교 수의과대학 수의미생물학연구실)
  • Received : 2018.05.08
  • Accepted : 2018.09.19
  • Published : 2018.09.30
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Abstract

In order for monitoring of pathogenic bacterial contamination in the freshly slaughtered poultry meats produced in Gyeong-Nam province, we first isolated 4 strains of Salmonella spp. and 32 strains of Enterococcus faecalis from the total 164 samples, then we analyzed potential virulence gene profiles of the bacterial isolates by PCR using species-specific primer. The potential virulence genes we selected in this study were stn, invA, fimA, spvR, and spvC for the isolates of Salmonella spp. and those of esp, cylM, cylA, cylB, gelE, fsrA, fsrB, and fsrC were for the isolates of E. faecalis. The PCR results showed that all 5 virulence genes were detected simultaneously in the all isolates of Salmonella spp. However, there was a diverse occurrence pattern of the virulence genes in the case of E. faecalis. The gene for enterococcal surface protein (esp) was not detected among the isolates (0/32), and the haemolysin gene prevalence rate of cylA, cylB, and cylM were 3.1% (1/32), 9.3% (3/32), and 9.3% (3/32), respectively. Moreover, the genes of gelE, fsrA, fsrB, and fsrC that associated with gelatinase activity were detected in the rate of 53.1% (17/32), 53.1% (17/32), 53.1% (17/32), and 53.1% (17/32), respectively. In conclusion, in the isolates of Salmonella spp., all possessed 5 virulence genes tested, suggesting that they are all related with each other clonally. However, in the case of E. faecalis isolates, the occurrence of the haemolysin genes (cylM, cylA, cylB) and the gelatinase genes (gelE, fsrABC) was highly variable among the isolates.

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