Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Genomic Features and Lytic Activity of the Bacteriophage PPPL-1 Effective against Pseudomonas syringae pv. actinidiae, a Cause of Bacterial Canker in Kiwifruit

  • Park, JungKum (Department of Horticultural Biotechnology, College of Life Science, Kyung Hee University) ;
  • Lim, Jeong-A (Research Group of Food Safety, Korea Food Research Institute) ;
  • Yu, Ji-Gang (Department of Horticultural Biotechnology, College of Life Science, Kyung Hee University) ;
  • Oh, Chang-Sik (Department of Horticultural Biotechnology, College of Life Science, Kyung Hee University)
  • Received : 2017.07.02
  • Accepted : 2018.07.20
  • Published : 2018.09.28
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Abstract

Bacterial canker in kiwifruit is caused by Pseudomonas syringae pv. actinidiae (Psa). In this study, the bacteriophage PPPL-1 effective against Psa was characterized. Belonging to the Podoviridae family, PPPL-1 was effective against most Psa strains as well as most Pseudomonas syringae pathovars. PPPL-1 carries a 41,149-bp genome with 49 protein coding sequences and is homologous to the previously reported phiPSA2 bacteriophage. The lytic activity of PPPL-1 was stable up to $40^{\circ}C$, within a range of pH 3-11 and under 365 nm UV light. These results indicate that the bacteriophage PPPL-1 might be useful to control Psa in the kiwifruit field.

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References

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