Laboraroty Animal Research

Korean Association for Laboratory Animal Science (한국실험동물학회)
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Mouse Cre-LoxP system: general principles to determine tissue-specific roles of target genes

  • Kim, Hyeonhui (Severance Biomedical Science Institute, Yonsei University College of Medicine) ;
  • Kim, Minki (Severance Biomedical Science Institute, Yonsei University College of Medicine) ;
  • Im, Sun-Kyoung (Severance Biomedical Science Institute, Yonsei University College of Medicine) ;
  • Fang, Sungsoon (Severance Biomedical Science Institute, Yonsei University College of Medicine)
  • Received : 2018.11.08
  • Accepted : 2018.12.05
  • Published : 2018.12.31
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Abstract

Genetically engineered mouse models are commonly preferred for studying the human disease due to genetic and pathophysiological similarities between mice and humans. In particular, Cre-loxP system is widely used as an integral experimental tool for generating the conditional. This system has enabled researchers to investigate genes of interest in a tissue/cell (spatial control) and/or time (temporal control) specific manner. A various tissue-specific Cre-driver mouse lines have been generated to date, and new Cre lines are still being developed. This review provides a brief overview of Cre-loxP system and a few commonly used promoters for expression of tissue-specific Cre recombinase. Also, we finally introduce some available links to the Web sites that provides detailed information about Cre mouse lines including their characterization.

Keywords

Acknowledgement

Supported by : National Research Foundation of Korea, Yonsei University College of Medicine

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