Food Engineering Progress (산업식품공학)

Korean Society for Industrial Food Engineering (한국산업식품공학회)
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Antioxidant and Anti-inflammatory Activities in Freeze-dried and Hot Air-dried Aronia (Aronia melanocarpa) Extracts

동결 및 열풍건조 아로니아 추출물의 항산화 및 항염증 활성

  • Park, Mi-Hye (Dept. of Food Science and Nutrition, Kyungpook National University) ;
  • Kim, Bumsik (Dept. of Food & Nutrition, Yeonsung University)
  • 박미혜 (경북대학교 식품영양학과) ;
  • 김범식 (연성대학교 식품영양과)
  • Received : 2018.09.06
  • Accepted : 2018.10.24
  • Published : 2018.11.30
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Abstract

This study examined the effects of drying methods of Aronia melanocarpa on contents of total polyphenols and flavonoids, antioxidant activity and anti-inflammatory activity. As a result, freeze-dried aronia extract (FDAE) significantly exhibited higher contents of total polyphenols and flavonoids (155.76 mg GAE/g and 105.70 mg QE/g) than hot-air dried aronia extract (HDAE) (134.93 mg GAE/g and 82.29 mg QE/g). Also, FDAE showed greater antioxidant activity than HDAE in both DPPH and ABTS. For anti-inflammatory activity, NO production from lipopolysaccharide activated RAW264.7 cell reduced at a dose-dependent manner in both FDAE and HDAE. However, reduction rate of NO production is higher in FDAE (62.7%) than in HDAE (33.5%). These results suggest that comparatively, freeze-drying is a better method for preserving the antioxidant and anti-inflammatory activities and of aronia.

본 연구에서는 열풍건조와 동결건조 2가지 방법으로 건조된 아로니아의 항산화 기능과 항염증 활성을 비교함으로써 아로니아의 최적 가공법과 기능성 소재로의 이용가능성을 살펴보고자 하였다. 동결건조는 $-18^{\circ}C$의 냉동고에서 8시간 냉동시킨 후, 동결건조기를 이용하여 $-55^{\circ}C$에서 48시간 동안 실시하였고, 열풍건조는 열풍건조기를 이용하여 $50^{\circ}C$에서 48시간 동안 수행하였다. 아로니아 추출은 분말화된 원료에 70% 에탄올을 10배수로 가하여 2시간 동안 $70^{\circ}C$로 연속 3회 환류추출하였다. 총 폴리페놀 함량은 동결건조 추출물이 155.76 mg GAE/g, 열풍건조 추출물이 134.93 mg GAE/g으로, 총 플라보노이드 함량은 동결건조 추출물이 105.7 mg QE/g, 열풍건조 추출물이 82.29 mg QE/g으로 분석되어 동결건조 추출물에서 더 높은 함량을 보였다(p<0.05). DPPH radical 소거 활성과 ABTS radical 소거 활성은 전 농도에 걸쳐 동결건조 추출물이 열풍건조 추출물에 비해 유의적으로 높은 것으로 나타나 동결건조 추출물이 열풍건조 추출물에 비해 우수한 항산화력을 가짐을 알 수 있었다. RAW 267.4를 이용한 MTT assay를 통해 세포독성을 조사한 결과 동결건조 및 열풍건조 아로니아 추출물 모두 100-300 ug/mL의 농도에서 세포독성은 나타나지 않았으며, 항염활성 평가에서는 동결건조 추출물이 300 ug/mL 농도에서 LPS 처리군에 비해 NO 생성을 62.7% 감소시킨 데 비해 열풍건조 추출물이 33.5%를 감소시켜 동결건조 추출물이 열풍건조 추출물에 비해 더 높은 항염증 활성을 보여주었다. 본 연구결과를 통해 열풍건조보다는 동결건조 방법이 아로니아의 유효성분 함량 증대를 통한 항산화 및 항염증 활성을 높이는 더 나은 방법임을 알 수 있었다. 단, 동결건조 방법이 산업적인 측면에서 장비 및 운용비용 등의 문제를 가진 만큼, 향후 열풍건조 대비 기능성분 및 기능성 개선과 함께 원가적인 측면을 고려하여 가공법을 결정할 필요가 있다.

Keywords

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