산업식품공학 (Food Engineering Progress)

  • 제22권4호
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  • Pages.315-320
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  • 2018
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  • 1226-4768(pISSN)
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  • 2288-1247(eISSN)
한국산업식품공학회 (Korean Society for Industrial Food Engineering)
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동결 및 열풍건조 아로니아 추출물의 항산화 및 항염증 활성

Antioxidant and Anti-inflammatory Activities in Freeze-dried and Hot Air-dried Aronia (Aronia melanocarpa) Extracts

  • 박미혜 (경북대학교 식품영양학과) ;
  • 김범식 (연성대학교 식품영양과)
  • Park, Mi-Hye (Dept. of Food Science and Nutrition, Kyungpook National University) ;
  • Kim, Bumsik (Dept. of Food & Nutrition, Yeonsung University)
  • 투고 : 2018.09.06
  • 심사 : 2018.10.24
  • 발행 : 2018.11.30
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구에서는 열풍건조와 동결건조 2가지 방법으로 건조된 아로니아의 항산화 기능과 항염증 활성을 비교함으로써 아로니아의 최적 가공법과 기능성 소재로의 이용가능성을 살펴보고자 하였다. 동결건조는 $-18^{\circ}C$의 냉동고에서 8시간 냉동시킨 후, 동결건조기를 이용하여 $-55^{\circ}C$에서 48시간 동안 실시하였고, 열풍건조는 열풍건조기를 이용하여 $50^{\circ}C$에서 48시간 동안 수행하였다. 아로니아 추출은 분말화된 원료에 70% 에탄올을 10배수로 가하여 2시간 동안 $70^{\circ}C$로 연속 3회 환류추출하였다. 총 폴리페놀 함량은 동결건조 추출물이 155.76 mg GAE/g, 열풍건조 추출물이 134.93 mg GAE/g으로, 총 플라보노이드 함량은 동결건조 추출물이 105.7 mg QE/g, 열풍건조 추출물이 82.29 mg QE/g으로 분석되어 동결건조 추출물에서 더 높은 함량을 보였다(p<0.05). DPPH radical 소거 활성과 ABTS radical 소거 활성은 전 농도에 걸쳐 동결건조 추출물이 열풍건조 추출물에 비해 유의적으로 높은 것으로 나타나 동결건조 추출물이 열풍건조 추출물에 비해 우수한 항산화력을 가짐을 알 수 있었다. RAW 267.4를 이용한 MTT assay를 통해 세포독성을 조사한 결과 동결건조 및 열풍건조 아로니아 추출물 모두 100-300 ug/mL의 농도에서 세포독성은 나타나지 않았으며, 항염활성 평가에서는 동결건조 추출물이 300 ug/mL 농도에서 LPS 처리군에 비해 NO 생성을 62.7% 감소시킨 데 비해 열풍건조 추출물이 33.5%를 감소시켜 동결건조 추출물이 열풍건조 추출물에 비해 더 높은 항염증 활성을 보여주었다. 본 연구결과를 통해 열풍건조보다는 동결건조 방법이 아로니아의 유효성분 함량 증대를 통한 항산화 및 항염증 활성을 높이는 더 나은 방법임을 알 수 있었다. 단, 동결건조 방법이 산업적인 측면에서 장비 및 운용비용 등의 문제를 가진 만큼, 향후 열풍건조 대비 기능성분 및 기능성 개선과 함께 원가적인 측면을 고려하여 가공법을 결정할 필요가 있다.

This study examined the effects of drying methods of Aronia melanocarpa on contents of total polyphenols and flavonoids, antioxidant activity and anti-inflammatory activity. As a result, freeze-dried aronia extract (FDAE) significantly exhibited higher contents of total polyphenols and flavonoids (155.76 mg GAE/g and 105.70 mg QE/g) than hot-air dried aronia extract (HDAE) (134.93 mg GAE/g and 82.29 mg QE/g). Also, FDAE showed greater antioxidant activity than HDAE in both DPPH and ABTS. For anti-inflammatory activity, NO production from lipopolysaccharide activated RAW264.7 cell reduced at a dose-dependent manner in both FDAE and HDAE. However, reduction rate of NO production is higher in FDAE (62.7%) than in HDAE (33.5%). These results suggest that comparatively, freeze-drying is a better method for preserving the antioxidant and anti-inflammatory activities and of aronia.

키워드

참고문헌

  1. Alessio HM. 1993. Oxygen-radical absorbance capacity assay for antioxidants. Free Radic. Biol. Med. 14: 303-11. https://doi.org/10.1016/0891-5849(93)90027-R
  2. Bermudez-Soto MJ, Tomas-Barberan FA. 2004. Evaluation of commercial red fruit juice concentrates as ingredients for antioxidant functional juices. Eur. Food Res. Technol. 219: 133-141. https://doi.org/10.1007/s00217-004-0940-3
  3. Blios MS. 1958. Antioxidant determination by the use of a stable free radical. Nature 181: 1199-1200. https://doi.org/10.1038/1811199a0
  4. Brinckerhoff CE, Rutter JL, Benbow U. 2000. Interstitial collagenases as markers of tumor progression. Clin. Cancer Res. 6: 4823-4830.
  5. Carmichael J, DeGraff WG, Gazdar AF, Minna JD, Mitchell JB. 1987. Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res. 47: 936-942.
  6. Charous SJ, Stricklin GP, Nanney LB, Burkey BB. 1997. Expression of matrix metalloproteinases and tissue inhibitor of metalloproteinases in head and neck squamous cell carcinoma. Ann. Oto. Rhino. 106: 271-278. https://doi.org/10.1177/000348949710600402
  7. Chen CC, Mo FE, Lau LF. 2001. The angiogenic factor Cyr61 activates a genetic program for wound healing in human skin fibroblasts. J. Biol. Chem. 276: 47329-47337. https://doi.org/10.1074/jbc.M107666200
  8. Choi KH, Oh HJ, Jeong YJ, Lim EJ, Han JS, Kim JH, Kim OY, Lee HS. 2015a. Physico-chemical analysis and antioxidant activities of Korea Aronia melanocarpa. J. Korean. Soc. Food Sci. Nutr. 44: 1165-1171. https://doi.org/10.3746/jkfn.2015.44.8.1165
  9. Choi MH, Jeon YJ, Shin HJ. 2015b. Anthocyanin analysis of pressure-extracted Korean blueberry juice and in vitro anti-inflammatory in RAW267.4 cell line. Korean Soc. Biotechnol. Bioeng. J. 30: 191-196.
  10. Chung HJ. 2014. Comparison of total polyphenols, total flavonoids, and biological activities of black chokeberry and blueberry cultivated in Korea. J. Korean Soc. Food Sci. Nutr. 43: 1349-1356. https://doi.org/10.3746/jkfn.2014.43.9.1349
  11. Curran, S. and Murray, G. 1999. Matrix metalloproteinases in tumor invation and metastasis. J. Pathol. 189: 300-308. https://doi.org/10.1002/(SICI)1096-9896(199911)189:3<300::AID-PATH456>3.0.CO;2-C
  12. Ezhilarasi PN, Indrani D, Jena BS, Anandharamakrishnan C. 2013. Freeze drying technique for microencapsulation of Garcinia fruit extract and its effect on bread quality. J. Food Eng. 117: 513-520. https://doi.org/10.1016/j.jfoodeng.2013.01.009
  13. Folin O, Denis W. 1912. On phosphotungstic-phosphomolybdic compounds as color reagents. J. Biol. Chem. 7: 239-243.
  14. Heinzemann A, Daser A. 2002. Mouse models for the genetic dissection of atopy. Int. Arch. Allergy Immunol. 127: 170-180. https://doi.org/10.1159/000053861
  15. Hong JH, Lee WY. 2004. Quality characteristics of osmotic dehydrated sweet pumpkin by different drying methods. J. Kor. Soc. Food Sci. Nutr. 33: 1573-1579. https://doi.org/10.3746/jkfn.2004.33.9.1573
  16. Hwang ES, Thi ND. 2014. Antioxidant contents and antioxidant activities of hot-water extracts of Aronia (Aronia melancocarpa) with different drying methods. Korean J. Food Sci. Technol. 46: 303-308. https://doi.org/10.9721/KJFST.2014.46.3.303
  17. Hwang SJ, Yoon WB, Lee OH, Cha SJ, Kim JD. 2014. Radical-scavenging-linked antioxidant activities of extracts from black chokeberry and blueberry cultivated in Korea. Food Chem. 146: 71-77. https://doi.org/10.1016/j.foodchem.2013.09.035
  18. Kim MJ, Kim IJ, Nam SY, Lee CH, Yun T, Song BH. 2006. Effects of drying methods on content of active components, antioxidant activity, and color values of Saururus chinensis Bail. Korean J. Medicinal Crop. Sci. 14: 8-13.
  19. Kim SY, Park KH, Moon HD, Jung SH, Lee SE, Kim BH. 2017. Anti-inflammatory effects of Acai berry ethanol extracts. J. Kor. Soc. Cosm. 23: 669-676.
  20. Kurozawa LE, Terng I, Hubinger MD, Park KJ. 2014. Ascorbic acid degradation of papaya during drying: Effect of process conditions and glass transition phenomenon. J. Food Eng. 123: 157-164. https://doi.org/10.1016/j.jfoodeng.2013.08.039
  21. Lee SJ, Kim EK, Kim YS, Hwang JW, Lee KH, Choi DK, Kang H, Moon SH, Jeon BT, Park PJ . 2012. Purification and characterization of a nitric oxide inhibitory peptide from Ruditapes philippinarum. Food Chem. Toxicol. 50:1660-1666. https://doi.org/10.1016/j.fct.2012.02.021
  22. Lim HJ, Lee HJ, Lim MH. 2015. Antioxidant activity of acaiberry, blueberry, corni, and mulberry. Kor. J. Aesthet. Cosmetol. 13: 445-452.
  23. Matchett MD, MacKinnon SL, Sweeney MI, GottschallPass KT, Hurta RA. 2006. Inhibition of matrix metalloproteinase activity in DU145 human prostate cancer 56 cells by flavonoids from lowbush blueberry (Vaccinium angustifolium): Possible roles for protein kinase C and mitogen activated protein-kinasemediated events. J. Nutr. Biochem.17: 117-125. https://doi.org/10.1016/j.jnutbio.2005.05.014
  24. Moreno MI, Isla MI, Sampietro AR, Vattuone MA. 2000. Comparison of the free radical-scavenging activity of propolis several regions of Argentina. J. Ethnopharmacology. 71: 109-114. https://doi.org/10.1016/S0378-8741(99)00189-0
  25. Park EJ, Ahn JJ, Kim JS, Kwon JH. 2013. Antioxidant activities in freeze-dried and hot air-dried Schizandra fruit (Schizandra chinensis Baillon) at different microwave-assisted extraction conditions. Korean J. Food Sci. Techonol. 45: 667-674. https://doi.org/10.9721/KJFST.2013.45.6.667
  26. Peleg H, Naim M, Rouseff RL, Zehavi. 1991. Distribution of bound and free phenolic acids in oranges (Citrus sinensis) and Grapefruits (Citrus paradisi). J. Sci. Food Agr. 57: 417-426. https://doi.org/10.1002/jsfa.2740570312
  27. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 26: 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  28. Wu XL, Gu LW, Prior RL, McKay S. 2004. Characterization of anthocyanins and proanthocyanidins in some cultivars of Ribes, Aronia, and Sambucus and their antioxidant capacity. J. Agric. Food Chem. 52: 7846-7856. https://doi.org/10.1021/jf0486850
  29. Yook HS, Kim KH, Jang SA. 2010. Quality characteristics of grape pomace with different drying methods. J. Korean Soc. Food Sci. Nutr. 39: 1353-1358. https://doi.org/10.3746/jkfn.2010.39.9.1353

피인용 문헌

  1. Quality Characteristics of Aronia Vinegar Imparted by Varying Concentrations of Seed Vinegar vol.50, pp.5, 2021, https://doi.org/10.3746/jkfn.2021.50.5.522