Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Development of an Efficient Bioassay Method for Testing Resistance to Bacterial Soft Rot of Radish

효과적인 무 무름병 저항성 검정법 개발

  • Lee, Soo Min (Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology) ;
  • Choi, Yong Ho (Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology) ;
  • Jang, Kyoung Soo (Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology) ;
  • Kim, Hun (Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology) ;
  • Lee, Seon-Woo (Department of Applied Bioscience, Dong-A University) ;
  • Choi, Gyung Ja (Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology)
  • 이수민 (한국화학연구원 친환경신물질연구센터) ;
  • 최용호 (한국화학연구원 친환경신물질연구센터) ;
  • 장경수 (한국화학연구원 친환경신물질연구센터) ;
  • 김헌 (한국화학연구원 친환경신물질연구센터) ;
  • 이선우 (동아대학교 응용생물공학과) ;
  • 최경자 (한국화학연구원 친환경신물질연구센터)
  • Received : 2018.08.02
  • Accepted : 2018.08.22
  • Published : 2018.09.30
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Abstract

Pectobacterium carotovorum subsp. carotovorum (Pcc) causes bacterial soft rot on a wide range of crops worldwide, especially in countries with warm and humid climates. This study was conducted to establish an efficient screening method for resistant cultivars of radish (Raphanus sativus) to bacterial soft rot. Resistance degrees of 60 commercial radish cultivars to the Pcc KACC 10421 isolate were investigated. For further study, six radish cultivars (Awooriwoldong, YR Championyeolmu, Jeonmuhumu, Bitgoeunyeolmu, Sunbongaltari, Housecheongok) showing different level of resistance to the bacterium were selected. The development of bacterial soft rot on the cultivars was tested according to several conditions such as incubation temperature, seedling stage of radish, inoculum concentration to develop the disease. On the basis of the results, we suggest that an efficient screening method for resistant radish to Pcc is to inoculate twenty-day-old seedlings with a bacterial suspension of Pcc at a concentration of $8{\times}10^5cfu/ml$ and then to cultivate the plants in a growth room at $25^{\circ}C$ and 80% RH with 12-hour light per day.

Pactobacterium carotovorum subsp. carotovorum (Pcc)에 의한 무름병은 전세계적으로 문제가 되고 있고 특히 온난 다습한 지역에서 문제가 심각하다. 본 연구는 Pcc에 의해 발생하는 무 무름병에 대한 효율적인 저항성 검정법을 확립하기 위하여 수행되었다. 시판 무 품종 60개의 Pcc에 대한 저항성 정도를 조사하고, 추후 실험을 위해 저항성에 차이를 보이는 6개 품종을 선발하였다. 이들 6개 품종의 접종하는 무의 생육 시기, 접종원 농도, 접종 후 재배 온도 등의 발병 조건에 따른 무름병 발생을 조사하였다. 이들 실험의 결과로부터 무의 무름병에 대한 저항성을 검정하기 위해서는, 무 종자를 파종하고 온실($25^{\circ}C{\pm}5^{\circ}C$)에서 20일 동안 재배한 유묘에 Pcc 균주의 세균현탁액($8{\times}10^5cfu/ml$)을 분무하여 접종하고, 접종한 식물은 $25^{\circ}C$ 습실상에 24시간 동안 배양한 후에 $25^{\circ}C$, 상대습도 80%의 생육상으로 이동하여 재배하는 것을 제안하고자 한다.

Keywords

References

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