The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Isolation and Identification of Fungal Species from the Insect Pest Tribolium castaneum in Rice Processing Complexes in Korea

  • Yun, Tae-Seong (Department of Plant Medicine, College of Life Science and Natural Resources, Sunchon National University) ;
  • Park, Sook-Young (Department of Plant Medicine, College of Life Science and Natural Resources, Sunchon National University) ;
  • Yu, Jihyun (Department of Plant Medicine, College of Life Science and Natural Resources, Sunchon National University) ;
  • Hwang, Yujin (Department of Plant Medicine, College of Life Science and Natural Resources, Sunchon National University) ;
  • Hong, Ki-Jeong (Department of Plant Medicine, College of Life Science and Natural Resources, Sunchon National University)
  • Received : 2018.02.13
  • Accepted : 2018.07.02
  • Published : 2018.10.01
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Abstract

The red flour beetle, Tribolium castaneum, is one of the most common and economically important pests of stored cereal products worldwide. Furthermore, these beetles can act as vectors for several fungal post-harvest diseases. In this study, we collected T. castaneum from 49 rice processing complexes (RPCs) nationwide during 2016-2017 and identified contaminating fungal species on the surface of the beetles. Five beetles from each region were placed on potato dextrose agar media or Fusarium selection media after wet processing with 100% relative humidity at $27^{\circ}C$ for one week. A total of 142 fungal isolates were thus collected. By sequence analysis of the internal transcribed spacer region, 23 fungal genera including one unidentified taxon were found to be associated with T. castaneum. The genus Aspergillus spp. (28.9%) was the most frequently present, followed by Cladosporium spp. (12.0%), Hyphopichia burtonii (9.2%), Penicillium spp. (8.5%), Mucor spp. (6.3%), Rhizopus spp. (5.6%), Cephaliophora spp. (3.5%), Alternaria alternata (2.8%) and Monascus sp. (2.8%). Less commonly identified were genera Fusarium, Nigrospora, Beauveria, Chaetomium, Coprinellus, Irpex, Lichtheimia, Trichoderma, Byssochlamys, Cochliobolus, Cunninghamella, Mortierella, Polyporales, Rhizomucor and Talaromyces. Among the isolates, two known mycotoxin-producing fungi, Aspergillus flavus and Fusarium spp. were also identified. This result is consistent with previous studies that surveyed fungal and mycotoxin contamination in rice from RPCs. Our study indicates that the storage pest, T. castaneum, would play an important role in spreading fungal contaminants and consequently increasing mycotoxin contamination in stored rice.

Keywords

References

  1. Abbas, H. K., Shier, W. T., Seo, J. A., Lee, Y. W. and Musser, S. M. 1998. Phytotoxicity and cytotoxicity of the fumonisin C and P series of mycotoxins from Fusarium spp. fungi. Toxicon 36:2033-2037. https://doi.org/10.1016/S0041-0101(98)00115-9
  2. Adams, J. M. 1977. A review of the literature concerning losses in stored cereals and pulses. Trop. Sci. 19:1-7.
  3. Barnett, H. L. and Hunter, B. B. 1972. Illustrated Genera of Imperfect Fungi. 3rd ed. Burgess Publishing Co., Minneapolis, Minnesota, USA. 241 pp.
  4. Birch, L. C. 1945. The influence of temperature, humidity and density on the oviposition of the small strain of Calandra oryzae L. and Rhizopertha dominica Fab (Coleoptera). Aust. J. Exp. Biol. Med. Sci. 23:197-203. https://doi.org/10.1038/icb.1945.31
  5. Bosly, H. A. and El-Banna, O. M. 2015. Isolation and identification of fungal growth on Tribolium castaneum in stored wheat flour. J. Entomol. Nematol. 7:11-17. https://doi.org/10.5897/JEN2015.0116
  6. Carbone, I. and Kohn, L. M. 1999. A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 91:553-556. https://doi.org/10.2307/3761358
  7. Daniels, N. E. 1956. Damage and reproduction by the flour beetles, Tribolium confusum and T. castaneum in wheat at three moistures. J. Econ. Entomol. 49:244-247. https://doi.org/10.1093/jee/49.2.244
  8. Fields, P., Bhadriraju, S. and Hulasare, R. 2012. Extreme temperatures. In: Stored Product Protection, eds. by D. W. Hagstrum, T. W. Philip and G. Cuperus, pp. 179-190. K-State Research and Extension, Manhattan, USA.
  9. Galvano, F., Piva, A., Ritieni, A. and Galvano, G. 2001. Dietary strategies to counteract the effects of mycotoxins: a review. J. Food. Prot. 64:120-131. https://doi.org/10.4315/0362-028X-64.1.120
  10. Glass, N. L. and Donaldson, G. C. 1995. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl. Environ. Microbiol. 61:1323-1330.
  11. Guerber, J. C., Liu, B., Correll, J. C. and Johnston, P. R. 2003. Characterization of diversity in Colletotrichum acutatum sensu lato by sequence analysis of two gene introns, mtDNA and intron RFLPs, and mating compatibility. Mycologia 95:872-895. https://doi.org/10.1080/15572536.2004.11833047
  12. Karunakaran, C., Jayas, D. S. and White, N. D. G. 2004. Identification of wheat kernels damaged by the red flour beetle using X-ray images. Biosyst. Eng. 87:267-274. https://doi.org/10.1016/j.biosystemseng.2003.12.002
  13. Kim, Y. B. and Yu, M. I. 1982. Activities of molds and insects during rice storage Part I. Activities of Tribolium castaneum Herbst and Aspergillus species. J. Korean Agric. Chem. Soc. 25:252-256 (in Korean).
  14. LeCato, G. L. and Flaherty, B. R. 1973. Tribolium castaneum progeny production and development on diet supplemented with egg of adult of Plodia interpunctella. J. Stored Prod. Res. 9:199-203. https://doi.org/10.1016/0022-474X(73)90028-3
  15. Lee, T., Lee, S. H., Lee, S. H., Shin, J. Y., Yun, J. C., Lee, Y. W. and Ryu, J. G. 2011. Occurrence of Fusarium mycotoxins in rice and its milling by-products in Korea. J. Food Prot. 74:1169-1174. https://doi.org/10.4315/0362-028X.JFP-10-564
  16. Lee, T., Lee, S., Kim, L. H. and Ryu, J. G. 2014. Occurrence of fungi and Fusarium mycotoxins in the rice samples from rice processing complex. Res. Plant Dis. 20:289-294 (in Korean). https://doi.org/10.5423/RPD.2014.20.4.289
  17. Miller, J. D. 1995. Fungi and mycotoxins in grain: implications for stored product research. J. Stored Prod. Res. 31:1-16. https://doi.org/10.1016/0022-474X(94)00039-V
  18. Nakaijima, T., Yoshida, M. and Tomimura, K. 2008. Effect of lodging on the level of mycotoxins in wheat, barley, and rice infected with the Fusarium graminearum species complex. J. Gen. Plant Pathol. 74:289-295. https://doi.org/10.1007/s10327-008-0103-7
  19. Nash, S. M. and Snyder, W. C. 1962. Quantitative estimations by plate counts of propagules of the bean root rot Fusarium in field soils. Phytopathology 52:567-572.
  20. O'donnell, K. and Cigelnik, E. 1997. Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the fungus Fusarium are nonorthologous. Mol. Phylogenet. Evol. 7:103-116. https://doi.org/10.1006/mpev.1996.0376
  21. O'donnell, K., Nirenberg, H. I., Aoki, T. and Cigelnik, E. 2000. A multigene phylogeny of the Gibberella fujikuroi species complex: detection of additional phylogenetically distinct species. Mycoscience 41:61-78. https://doi.org/10.1007/BF02464387
  22. Ostry, V. 2008. Alternaria mycotoxins: an overview of chemical characterization, producers, toxicity, analysis and occurrence in foodstuffs. World Mycotoxin J. 1:175-188. https://doi.org/10.3920/WMJ2008.x013
  23. Park, J. W., Choi, S. Y., Hwang, H. J. and Kim, Y. B. 2005. Fungal mycoflora and mycotoxins in Korean polished rice destined for humans. Int. J. Food Microbiol. 103:305-314. https://doi.org/10.1016/j.ijfoodmicro.2005.02.001
  24. Philip, T. W. and Throne, J. E. 2010. Biorational approaches for managing stored-product insect. Annu. Rev. Entomol. 55:375-397. https://doi.org/10.1146/annurev.ento.54.110807.090451
  25. Rahmani, A., Soleimany, F., Hosseini, H. and Nateghi, L. 2011. Survey on the occurrence of aflatoxins in rice from different provinces of Iran. Food Addit. Contam. B 4:185-190. https://doi.org/10.1080/19393210.2011.599865
  26. Reddy, K. R. N., Reddy, C. S., Abbas, H. K., Abel, C. A. and Muralidharan, K. 2008. Mycotoxigenic fungi, mycotoxins, and management of rice grains. Toxin Rev. 27:287-317. https://doi.org/10.1080/15569540802432308
  27. Roorda, F. A., Schulten, G. G. M. and Andriessen, E. A. M. 1982. Laboratory observations on the development of Tribolium castaneum Herbst (Col., Tenebrionidae) on millet at different temperatures and relative humidities. J. Appl. Entomol. 93:446-452.
  28. Paterson, R. R. M. and Lima, N. 2010. How will climate change affect mycotoxins in food? Food Res. Int. 43:1902-1914. https://doi.org/10.1016/j.foodres.2009.07.010
  29. Samson, R. A. and De Boer, E. 1995. Introduction to food-bornd fungi. 4th ed. Centraalbureau voor Schimmelcultures, Baarn, Netherlands. 299 pp.
  30. Shazali, M. E. H. and Smith, R. 1986. Life history studies of externally feeding pest of stored sorghum: Corcyra cephalonica (Staint.) and Tribolium castaneum (Hbst). J. Stored Prod. Res. 22:55-61. https://doi.org/10.1016/0022-474X(86)90019-6
  31. Simpanya, M. F., Allotey, J. and Mpuchane, S. 2001. Insect and mycoflora interactions in maize flour. Afr. J. Food Nut. Sci. 1:3-8.
  32. Son, S. W., Nam, Y. J., Lee, S. H., Lee, S. M., Lee, S. H., Kim, M. J., Lee, T., Yun, J. C. and Ryu, J. G. 2011. Toxigenic fungal contaminants in the 2009-harvested rice and its milling-by products samples collected from rice processing complexes in Korea. Res. Plant Dis. 17:280-287 (in Korean). https://doi.org/10.5423/RPD.2011.17.3.280
  33. Tanaka, K., Kobayashi, H., Nagata, T. and Manabe, M. 2004. Natural occurrence of trichothecenes on lodged and water damaged domestic rice in Japan. J. Food Hyg. Soc. Jpn. 45:63-66. https://doi.org/10.3358/shokueishi.45.63
  34. Tipples, K. H. 1995. Quality and nutritional changes in stored grain. In: Stored-grain ecosystems, eds. by D. S. Jayas, N. D. G. White and W. E. Muir, pp. 325-351. New York Marcel Dekker, New York, USA.
  35. White, T. J., Bruns, T. D., Lee, S. B. and Taylor, J. W. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics In: PCR Protocols: a guide to methods and application, eds. by M. A. Innis, D. H. Gelfand, J. J. Sninsky and T. J. White, pp. 315-322. Academic Press, New York, USA.