Plant Breeding and Biotechnology

The Korean Society of Breeding Science (한국육종학회)
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Single Nucleotide Polymorphism (SNP) Discovery and Kompetitive Allele-Specific PCR (KASP) Marker Development with Korean Japonica Rice Varieties

  • Cheon, Kyeong-Seong (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration (RDA)) ;
  • Baek, Jeongho (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration (RDA)) ;
  • Cho, Young-il (Seed Industry Promotion Center, Foundation of Agri. Tech. Commercialization & Transfer (FACT)) ;
  • Jeong, Young-Min (Seed Industry Promotion Center, Foundation of Agri. Tech. Commercialization & Transfer (FACT)) ;
  • Lee, Youn-Young (Seed Industry Promotion Center, Foundation of Agri. Tech. Commercialization & Transfer (FACT)) ;
  • Oh, Jun (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration (RDA)) ;
  • Won, Yong Jae (Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration (RDA)) ;
  • Kang, Do-Yu (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration (RDA)) ;
  • Oh, Hyoja (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration (RDA)) ;
  • Kim, Song Lim (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration (RDA)) ;
  • Choi, Inchan (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration (RDA)) ;
  • Yoon, In Sun (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration (RDA)) ;
  • Kim, Kyung-Hwan (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration (RDA)) ;
  • Han, Jung-Heon (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration (RDA)) ;
  • Ji, Hyeonso (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration (RDA))
  • Received : 2018.10.19
  • Accepted : 2018.10.24
  • Published : 2018.12.01
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Abstract

Genome resequencing by next-generation sequencing technology can reveal numerous single nucleotide polymorphisms (SNPs) within a closely-related cultivar group, which would enable the development of sufficient SNP markers for mapping and the identification of useful genes present in the cultivar group. We analyzed genome sequence data from 13 Korean japonica rice varieties and discovered 740,566 SNPs. The SNPs were distributed at 100-kbp intervals throughout the rice genome, although the SNP density was uneven among the chromosomes. Of the 740,566 SNPs, 1,014 SNP sites were selected on the basis of polymorphism information content (PIC) value higher than 0.4 per 200-kbp interval, and 506 of these SNPs were converted to Kompetitive Allele-Specific PCR (KASP) markers. The 506 KASP markers were tested for genotyping with the 13 sequenced Korean japonica rice varieties, and polymorphisms were detected in 400 KASP markers (79.1%) which would be suitable for genetic analysis and molecular breeding. Additionally, a genetic map comprising 205 KASP markers was successfully constructed with 188 $F_2$ progenies derived from a cross between the varieties, Junam and Nampyeong. In a phylogenetic analysis with 81 KASP markers, 13 Korean japonica varieties showed close genetic relationships and were divided into three groups. More KASP markers are being developed and these markers will be utilized in gene mapping, quantitative trait locus (QTL) analysis, marker-assisted selection and other strategies relevant to crop improvement.

Keywords

Acknowledgement

Supported by : Rural Development Administration

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