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Microsatellite Markers를 이용한 따오기의 유전적 특성 분석

Genetic analysis of endangered species Crested Ibis (Nipponia nippon) microsatellite markers

  • 투고 : 2018.08.20
  • 심사 : 2018.11.19
  • 발행 : 2018.12.31

초록

세계적으로 멸종위기종인 따오기 (Nipponia Nippon)는 한국에서도 멸종위기종으로 구분되어 있으며, 이를 복원하기 위해 2008년 10월에 중국에서 따오기 1쌍을 도입하여 한국 최초로 인공번식에 성공하였다. 이 후 우포늪 따오기 종 복원을 통해 2017년까지 개체수가 200마리 이상으로 늘어났다. 본 연구에서는 우포늪 따오기 228 개체를 대상으로 성별을 결정하고, microsatellite 마커를 이용하여 유전적 유연관계를 분석하였다. 그 결과, 115마리의 암컷과 113마리의 수컷으로 판별되었으며, 2016년보다 2017년에 서식하고 있는 개체의 이형접합도와 다형성 정보지수가 모두 감소한 것으로 나타났다. 이는 작은 집단으로부터 개체 수를 늘려나가다 보니 2017년에 근친율이 증가한 것으로 사료된다. 향 후 본 연구는 한국 따오기의 번식사업 및 복원사업을 위한 기초자료로 유용하게 활용될 것으로 기대된다.

The Korean Crested ibis Nipponia Nippon is an endangered species. A pair of Crested ibis was introduced from China in October 2008, and a successful program of artificial incubation of the species, and over 200 animals have been successfully bred through the restoration project up to 2017 at Upo ibis restoration center. We assessed genetic diversity and sex determination in the Korean Crested ibis. In total, 228 Crested ibis (115 females and 113 males) were identified. And genetic diversity measures, observed heterozygosity, expected heterozygosity, and polymorphic information content values were lower in 2017 than those in 2016. The inbreeding coefficient showed that the degree of ancestry increased in 2017. The decrease in polymorphism and increase in the degree of ancestry is thought to be due to inbreeding in such a small group. In this study provided important insight into protocols for genetic management of the breeding population of Korean Crested ibis in Korea and will help in extending the restoration program.

키워드

과제정보

연구 과제 주관 기관 : Hankyong National University

참고문헌

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피인용 문헌

  1. Development and Characterization, and Application of Ten Polymorphic Microsatellite Markers in the Crested Ibis Nipponia nippon from South Korea vol.36, pp.2, 2018, https://doi.org/10.5635/ased.2020.36.2.029